PMID- 26560942
OWN - NLM
STAT- MEDLINE
DCOM- 20161031
LR  - 20200930
IS  - 1555-8576 (Electronic)
IS  - 1538-4047 (Print)
IS  - 1538-4047 (Linking)
VI  - 17
IP  - 1
DP  - 2016
TI  - Menin localization in cell membrane compartment.
PG  - 114-22
LID - 10.1080/15384047.2015.1108497 [doi]
AB  - Menin is encoded by the MEN1 gene, which is mutated in an inherited human 
      syndrome, multiple endocrine neoplasia type 1(MEN1). Menin is primarily nuclear 
      protein, acting as a tumor suppressor in endocrine organs, but as an oncogenic 
      factor in the mixed lineage leukemia, in a tissue-specific manner. Recently, the 
      crystal structures of menin with different binding partners reveal menin as a key 
      scaffold protein that functionally interacts with various partners to regulate 
      gene transcription in the nucleus. However, outside the nucleus, menin also 
      regulates multiple signaling pathways that traverse the cell surface membrane. 
      The precise nature regarding to how menin associates with the membrane fraction 
      is poorly understood. Here we show that a small fraction of menin associates with 
      the cell membrane fraction likely via serine palmitoylation. Moreover, the 
      majority of the membrane-associated menin may reside inside membrane vesicles, as 
      menin is protected from trypsin-mediated proteolysis, but disruption of the 
      membrane fraction using detergent abolishes the detection. Consistently, cellular 
      staining for menin also reveals the distribution of menin in the cell membrane 
      and the punctate-like cell organelles. Our findings suggest that part of 
      intracellular menin associates with the cell membrane peripherally as well as 
      resides within the membrane vesicles.
FAU - He, Xin
AU  - He X
AD  - a Abramson Family Cancer Research Institute, Department of Cancer Biology, 
      Abramson Cancer Center, University of Pennsylvania Perelman School of Medicine , 
      421 Curie Blvd., Philadelphia , PA 19104 , USA.
FAU - Wang, Lei
AU  - Wang L
AD  - a Abramson Family Cancer Research Institute, Department of Cancer Biology, 
      Abramson Cancer Center, University of Pennsylvania Perelman School of Medicine , 
      421 Curie Blvd., Philadelphia , PA 19104 , USA.
AD  - d Department of Urology , Renmin Hospital of Wuhan University , Wuhan 430060 , 
      Hubei , China.
FAU - Yan, Jizhou
AU  - Yan J
AD  - b Department of Biology and Biotechnology , Shanghai Ocean University , 999 
      Hucheng Ring Rd Lingang New City, Shanghai , 201306 , China.
FAU - Yuan, Chaoxing
AU  - Yuan C
AD  - c The Proteomics and Systems Facility, Department of Pharmacology, University of 
      Pennsylvania Perelman School of Medicine , Philadelphia, 421 Curie Blvd., 
      Philadelphia , PA 19104 , USA.
FAU - Witze, Eric S
AU  - Witze ES
AD  - a Abramson Family Cancer Research Institute, Department of Cancer Biology, 
      Abramson Cancer Center, University of Pennsylvania Perelman School of Medicine , 
      421 Curie Blvd., Philadelphia , PA 19104 , USA.
FAU - Hua, Xianxin
AU  - Hua X
AD  - a Abramson Family Cancer Research Institute, Department of Cancer Biology, 
      Abramson Cancer Center, University of Pennsylvania Perelman School of Medicine , 
      421 Curie Blvd., Philadelphia , PA 19104 , USA.
LA  - eng
GR  - 1-R01-CA-178856/CA/NCI NIH HHS/United States
GR  - R01-DK085121/DK/NIDDK NIH HHS/United States
GR  - R01 DK097555/DK/NIDDK NIH HHS/United States
GR  - R01 CA178856/CA/NCI NIH HHS/United States
GR  - P30DK050306/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Cancer Biol Ther
JT  - Cancer biology & therapy
JID - 101137842
RN  - 0 (MEN1 protein, human)
RN  - 0 (Nuclear Matrix-Associated Proteins)
RN  - 0 (Proto-Oncogene Proteins)
RN  - 452VLY9402 (Serine)
SB  - IM
MH  - Animals
MH  - Cell Membrane/genetics/*metabolism
MH  - Cell Nucleus/genetics/*metabolism
MH  - Crystallography, X-Ray
MH  - Humans
MH  - Lipoylation
MH  - Mice
MH  - Nuclear Matrix-Associated Proteins/chemistry/genetics
MH  - Proto-Oncogene Proteins/chemistry/genetics/*metabolism
MH  - Rats
MH  - Serine/metabolism
MH  - Signal Transduction/genetics
PMC - PMC4847924
OTO - NOTNLM
OT  - Cell membrane
OT  - menin
OT  - palmitoylation
OT  - secretory pathway
EDAT- 2015/11/13 06:00
MHDA- 2016/11/01 06:00
PMCR- 2016/11/11
CRDT- 2015/11/13 06:00
PHST- 2015/11/13 06:00 [entrez]
PHST- 2015/11/13 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
PHST- 2016/11/11 00:00 [pmc-release]
AID - 1108497 [pii]
AID - 10.1080/15384047.2015.1108497 [doi]
PST - ppublish
SO  - Cancer Biol Ther. 2016;17(1):114-22. doi: 10.1080/15384047.2015.1108497.