PMID- 26566284
OWN - NLM
STAT- MEDLINE
DCOM- 20160629
LR  - 20191210
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 10
IP  - 11
DP  - 2015
TI  - Reduced Contextual Discrimination following Alcohol Consumption or MDMA 
      Administration in Mice.
PG  - e0142978
LID - 10.1371/journal.pone.0142978 [doi]
LID - e0142978
AB  - The recreational drugs, alcohol and 3,4-Methylenedioxymethamphetamine (MDMA, 
      "Ecstasy") have both been shown to cause immune activation in vivo, and they are 
      linked to cognitive impairment and anxiety-like behaviors in rodents. The 
      neuronal effects of these drugs in the hippocampal area, an area that has been a 
      focus of studies aiming to explain the mechanisms underlying anxiety 
      related-disorders, remains poorly understood. Therefore we investigated the 
      specific inflammatory impact of alcohol and MDMA on this area of the brain and on 
      a hippocampal-related behavioral task. We centered our study on two inflammatory 
      factors linked to anxiety-related disorders, namely Interleukin-1beta (IL-1beta) and 
      brain-derived neurotrophic factor (BDNF). We subjected drug-consuming mice to a 
      battery of behavioral tests to evaluate general activity, anxiety-like and 
      depressive-live behaviors. We then introduced them to a contextual fear 
      discrimination task and immune-related effects were examined by 
      immunohistochemical and biochemical studies. Our results suggest that there is a 
      relationship between the induction of immune activated pathways by voluntary 
      alcohol consumption and a high-dose MDMA. Furthermore, the ability of mice to 
      perform a contextual fear discrimination task was impaired by drug consumption 
      and we report long term inflammatory alterations in the hippocampus even several 
      weeks after drug intake. This information will be helpful for discovering new 
      selective drug targets, and to develop treatments and preventive approaches for 
      patients with anxiety-related disorders.
FAU - Johansson, Emily M
AU  - Johansson EM
AD  - Neurobiology of Behaviour Research Group (GReNeC), Department of Health and 
      Experimental Sciences, Universitat Pompeu Fabra, IMIM, Hospital del Mar Medical 
      Research Institute, Barcelona Biomedical Research Park C/Dr. Aiguader 88, 08003, 
      Barcelona, Spain.
FAU - Garcia-Gutierrez, Maria S
AU  - Garcia-Gutierrez MS
AD  - Instituto de Neurociencias, Universidad Miguel Hernandez-CSIC, Avda. Ramon y 
      Cajal s/n, San Juan de Alicante, Alicante, Spain.
AD  - Red Tematica de Investigacion Cooperativa en Salud (RETICS), Red de Trastornos 
      Adictivos, Instituto de Salud Carlos III, Madrid, Spain.
FAU - Moscoso-Castro, Maria
AU  - Moscoso-Castro M
AD  - Neurobiology of Behaviour Research Group (GReNeC), Department of Health and 
      Experimental Sciences, Universitat Pompeu Fabra, IMIM, Hospital del Mar Medical 
      Research Institute, Barcelona Biomedical Research Park C/Dr. Aiguader 88, 08003, 
      Barcelona, Spain.
FAU - Manzanares, Jorge
AU  - Manzanares J
AD  - Instituto de Neurociencias, Universidad Miguel Hernandez-CSIC, Avda. Ramon y 
      Cajal s/n, San Juan de Alicante, Alicante, Spain.
AD  - Red Tematica de Investigacion Cooperativa en Salud (RETICS), Red de Trastornos 
      Adictivos, Instituto de Salud Carlos III, Madrid, Spain.
FAU - Valverde, Olga
AU  - Valverde O
AD  - Neurobiology of Behaviour Research Group (GReNeC), Department of Health and 
      Experimental Sciences, Universitat Pompeu Fabra, IMIM, Hospital del Mar Medical 
      Research Institute, Barcelona Biomedical Research Park C/Dr. Aiguader 88, 08003, 
      Barcelona, Spain.
AD  - Red Tematica de Investigacion Cooperativa en Salud (RETICS), Red de Trastornos 
      Adictivos, Instituto de Salud Carlos III, Madrid, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151113
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Hallucinogens)
RN  - 0 (IL1B protein, mouse)
RN  - 0 (Illicit Drugs)
RN  - 0 (Interleukin-1beta)
RN  - KE1SEN21RM (N-Methyl-3,4-methylenedioxyamphetamine)
SB  - IM
MH  - *Alcohol Drinking
MH  - Animals
MH  - Anxiety/drug therapy
MH  - Behavior, Animal/drug effects
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Cognition/drug effects
MH  - Discrimination Learning/*drug effects
MH  - Fear/drug effects
MH  - Gene Expression Profiling
MH  - Gene Expression Regulation/*drug effects
MH  - Hallucinogens/adverse effects
MH  - Hippocampus/drug effects
MH  - Illicit Drugs/chemistry
MH  - Immunohistochemistry
MH  - Inflammation
MH  - Interleukin-1beta/*metabolism
MH  - Male
MH  - Maze Learning
MH  - Mice
MH  - Motor Activity/drug effects
MH  - N-Methyl-3,4-methylenedioxyamphetamine/*adverse effects
MH  - Neurons/drug effects
MH  - Polymerase Chain Reaction
PMC - PMC4643963
COIS- Competing Interests: The authors declare no conflicts of interest.
EDAT- 2015/11/14 06:00
MHDA- 2016/06/30 06:00
PMCR- 2015/11/13
CRDT- 2015/11/14 06:00
PHST- 2015/04/09 00:00 [received]
PHST- 2015/10/29 00:00 [accepted]
PHST- 2015/11/14 06:00 [entrez]
PHST- 2015/11/14 06:00 [pubmed]
PHST- 2016/06/30 06:00 [medline]
PHST- 2015/11/13 00:00 [pmc-release]
AID - PONE-D-15-15011 [pii]
AID - 10.1371/journal.pone.0142978 [doi]
PST - epublish
SO  - PLoS One. 2015 Nov 13;10(11):e0142978. doi: 10.1371/journal.pone.0142978. 
      eCollection 2015.