PMID- 26567303
OWN - NLM
STAT- MEDLINE
DCOM- 20161012
LR  - 20181113
IS  - 1098-5336 (Electronic)
IS  - 0099-2240 (Print)
IS  - 0099-2240 (Linking)
VI  - 82
IP  - 2
DP  - 2016 Jan 15
TI  - "Candidatus Finniella" (Rickettsiales, Alphaproteobacteria), Novel Endosymbionts 
      of Viridiraptorid Amoeboflagellates (Cercozoa, Rhizaria).
PG  - 659-70
LID - 10.1128/AEM.02680-15 [doi]
AB  - The Rickettsiales (Alphaproteobacteria) are obligate intracellular bacteria that 
      colonize a wide range of eukaryotic hosts, including diverse metazoa and 
      protists. Here, we characterize rickettsial endosymbionts discovered in the 
      cytoplasm of the algivorous amoeboflagellates Viridiraptor invadens and 
      Orciraptor agilis (Viridiraptoridae, Cercozoa, Rhizaria), supplying evidence of 
      free-living, phagotrophic members of the Cercozoa serving as hosts for 
      Rickettsiales. According to 16S rRNA gene phylogenies, the bacteria represent two 
      closely related but distinct genotypes within a deep-branching rickettsial clade, 
      which contains the genera "Candidatus Odyssella," "Candidatus Paracaedibacter," 
      and "Candidatus Captivus." Using the full-cycle rRNA approach, we detected the 
      novel bacteria in four of nine viridiraptorid strains tested. Furthermore, two 
      specific oligonucleotide probes with a single-nucleotide-difference discriminated 
      both bacterial genotypes by fluorescence in situ hybridization (FISH). We 
      establish the candidate species "Candidatus Finniella inopinata" (found in 
      Viridiraptor invadens) and "Candidatus Finniella lucida" (found in Orciraptor 
      agilis) for the novel bacteria and propose a new, provisional family of 
      Rickettsiales, "Candidatus Paracaedibacteraceae."
CI  - Copyright (c) 2016, American Society for Microbiology. All Rights Reserved.
FAU - Hess, Sebastian
AU  - Hess S
AD  - Department of Botany, Cologne Biocenter, University of Cologne, Cologne, Germany 
      shess@magniflash.de.
FAU - Suthaus, Andreas
AU  - Suthaus A
AD  - Department of Botany, Cologne Biocenter, University of Cologne, Cologne, Germany.
FAU - Melkonian, Michael
AU  - Melkonian M
AD  - Department of Botany, Cologne Biocenter, University of Cologne, Cologne, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151113
PL  - United States
TA  - Appl Environ Microbiol
JT  - Applied and environmental microbiology
JID - 7605801
SB  - IM
MH  - Alphaproteobacteria/classification/genetics/*isolation & purification/*physiology
MH  - Cercozoa/*microbiology/physiology
MH  - Cytoplasm/microbiology
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - *Symbiosis
PMC - PMC4711129
EDAT- 2015/11/15 06:00
MHDA- 2016/10/13 06:00
PMCR- 2016/07/07
CRDT- 2015/11/15 06:00
PHST- 2015/08/18 00:00 [received]
PHST- 2015/11/08 00:00 [accepted]
PHST- 2015/11/15 06:00 [entrez]
PHST- 2015/11/15 06:00 [pubmed]
PHST- 2016/10/13 06:00 [medline]
PHST- 2016/07/07 00:00 [pmc-release]
AID - AEM.02680-15 [pii]
AID - 02680-15 [pii]
AID - 10.1128/AEM.02680-15 [doi]
PST - epublish
SO  - Appl Environ Microbiol. 2015 Nov 13;82(2):659-70. doi: 10.1128/AEM.02680-15. 
      Print 2016 Jan 15.