PMID- 26567448
OWN - NLM
STAT- MEDLINE
DCOM- 20160311
LR  - 20151116
IS  - 1078-6791 (Print)
IS  - 1078-6791 (Linking)
VI  - 21
IP  - 6
DP  - 2015 Nov-Dec
TI  - Brain-derived Neurotrophic Factor Signaling Pathway: Modulation by Acupuncture in 
      Telomerase Knockout Mice.
PG  - 36-46
AB  - CONTEXT: Telomerase is a critical enzyme that is involved in aging and cancer and 
      that is thought to be a part of multiple neurological diseases. OBJECTIVE: To 
      investigate the telomerase response in the brain to acupuncture, the study 
      examined the levels of expression of brain-derived neurotrophic factor (BDNF) and 
      its downstream signaling molecules, including tyrosine kinase receptor Beta (TrkB), 
      p75 neurotrophin receptor (p75NTR), protein kinase B (Akt), extracellular 
      signal-regulated protein kinase (ERK1/2), and nuclear factor kappaBeta (NF-kappaBeta). DESIGN: 
      Both telomerase-deficient (Terc(-)/(-)) mice (Terc(-)/(-) group) and normal, wild-type 
      (WT) mice (WT group) were randomly assigned to 1 of 3 subgroups, 1 receiving 
      acupuncture (acupuncture subgroup), 1 receiving sham acupuncture therapy (sham 
      subgroup), and 1 receiving no treatment (control subgroup). SETTING: The study 
      occurred at the University of South Florida Health Byrd Alzheimer's Institute 
      (Tampa, FL, USA). INTERVENTION: The 2 acupuncture subgroups received acupuncture 
      at the stomach 36 (ST-36) position for 30 min/d for 4 d. For the 2 sham groups, 
      the sham point was set at a location approximately 3 mm to the lateral side of 
      the tail on the gluteus muscle following the same schedule. OUTCOME MEASURES: 
      After 4 d, the mice were sacrificed, and the brain tissues were collected. The 
      protein levels in the hippocampus and dentate gyrus (DG) of each mouse were 
      determined by western blotting and immunostaining assays. RESULTS: The Terc(-)/(-) 
      group showed downregulated hippocampal BDNF expression compared with the WT mice. 
      Acupuncture at ST-36 for 4 d upregulated BDNF, TrkB, p75NTR, Akt, and ERK1/2 in 
      the DG and hippocampus of the telomerase-deficient mice, but that result was not 
      seen in the WT mice with normally functioning telomerase. CONCLUSIONS: The use of 
      acupuncture in pathologies associated with telomerase deficiencies, such as 
      Alzheimer's disease (AD) and Parkinson's disease (PD), may provide some benefit 
      in terms of eliciting better clinical responses. The research team believes that 
      result occurs through the activation of BDNF and its downstream signaling 
      pathways in populations of patients who exhibit low telomerase activity.
FAU - Lin, Dong
AU  - Lin D
FAU - Wu, Qiang
AU  - Wu Q
FAU - Lin, Xiaoyang
AU  - Lin X
FAU - Borlongan, Cesar V
AU  - Borlongan CV
FAU - He, Zhi-Xu
AU  - He ZX
FAU - Tan, Jun
AU  - Tan J
FAU - Cao, Chuanhai
AU  - Cao C
FAU - Zhou, Shu-Feng
AU  - Zhou SF
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Altern Ther Health Med
JT  - Alternative therapies in health and medicine
JID - 9502013
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (telomerase RNA)
RN  - 63231-63-0 (RNA)
RN  - EC 2.7.10.1 (Receptor Protein-Tyrosine Kinases)
RN  - EC 2.7.7.49 (Telomerase)
SB  - IM
MH  - Acupuncture Therapy/*methods
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/*metabolism
MH  - Mice
MH  - Mice, Knockout
MH  - RNA/*metabolism
MH  - Random Allocation
MH  - Receptor Protein-Tyrosine Kinases/*metabolism
MH  - Signal Transduction
MH  - Telomerase/*metabolism
EDAT- 2015/11/17 06:00
MHDA- 2016/03/12 06:00
CRDT- 2015/11/17 06:00
PHST- 2015/11/17 06:00 [entrez]
PHST- 2015/11/17 06:00 [pubmed]
PHST- 2016/03/12 06:00 [medline]
PST - ppublish
SO  - Altern Ther Health Med. 2015 Nov-Dec;21(6):36-46.