PMID- 26572927
OWN - NLM
STAT- MEDLINE
DCOM- 20161219
LR  - 20161230
IS  - 1523-4681 (Electronic)
IS  - 0884-0431 (Linking)
VI  - 31
IP  - 4
DP  - 2016 Apr
TI  - Dietary Restriction-Induced Alterations in Bone Phenotype: Effects of Lifelong 
      Versus Short-Term Caloric Restriction on Femoral and Vertebral Bone in C57BL/6 
      Mice.
PG  - 852-63
LID - 10.1002/jbmr.2745 [doi]
AB  - Caloric restriction (CR) is a well-described dietary intervention that delays the 
      onset of aging-associated biochemical and physiological changes, thereby 
      extending the life span of rodents. The influence of CR on metabolism, strength, 
      and morphology of bone has been controversially discussed in literature. Thus, 
      the present study evaluated whether lifelong CR versus short-term late-onset 
      dietary intervention differentially affects the development of senile 
      osteoporosis in C57BL/6 mice. Two different dietary regimens with 40% food 
      restriction were performed: lifelong CR starting in 4-week-old mice was 
      maintained for 4, 20, or 74 weeks. In contrast, short-term late-onset CR lasting 
      a period of 12 weeks was commenced at 48 or 68 weeks of age. Control mice were 
      fed ad libitum (AL). Bone specimens were assessed using microcomputed tomography 
      (muCT, femur and lumbar vertebral body) and biomechanical testing (femur). Adverse 
      effects of CR, including reduced cortical bone mineral density (Ct.BMD) and 
      thickness (Ct.Th), were detected to some extent in senile mice (68+12w) but in 
      particular in cortical bone of young growing mice (4+4w), associated with reduced 
      femoral failure force (F). However, we observed a profound capacity of bone to 
      compensate these deleterious changes of minor nutrition with increasing age 
      presumably via reorganization of trabecular bone. Especially in lumbar vertebrae, 
      lifelong CR lasting 20 or 74 weeks had beneficial effects on trabecular bone 
      mineral density (Tb.BMD), bone volume fraction (BV/TV), and trabecular number 
      (Tb.N). In parallel, lifelong CR groups showed reduced structure model index 
      values compared to age-matched controls indicating a transformation of vertebral 
      trabecular bone microarchitecture toward a platelike geometry. This effect was 
      not visible in senile mice after short-term 12-week CR. In summary, CR has 
      differential effects on cortical and trabecular bone dependent on bone 
      localization and starting age. Our study underlines that bone compartments 
      possess a lifelong capability to cope with changing nutritional influences.
CI  - (c) 2015 American Society for Bone and Mineral Research.
FAU - Behrendt, Ann-Kathrin
AU  - Behrendt AK
AD  - Department of Trauma, Hand, and Reconstructive Surgery, Rostock University 
      Medical Center, Rostock, Germany.
AD  - Institute for Experimental Surgery, Rostock University Medical Center, Rostock, 
      Germany.
FAU - Kuhla, Angela
AU  - Kuhla A
AD  - Institute for Experimental Surgery, Rostock University Medical Center, Rostock, 
      Germany.
FAU - Osterberg, Anja
AU  - Osterberg A
AD  - Institute of Immunology, Rostock University Medical Center, Rostock, Germany.
FAU - Polley, Christian
AU  - Polley C
AD  - Institute for Experimental Surgery, Rostock University Medical Center, Rostock, 
      Germany.
FAU - Herlyn, Philipp
AU  - Herlyn P
AD  - Department of Trauma, Hand, and Reconstructive Surgery, Rostock University 
      Medical Center, Rostock, Germany.
FAU - Fischer, Dagmar-Christiane
AU  - Fischer DC
AD  - Department of Pediatrics, Rostock University Medical Center, Rostock, Germany.
FAU - Scotland, Maike
AU  - Scotland M
AD  - Department of Trauma, Hand, and Reconstructive Surgery, Rostock University 
      Medical Center, Rostock, Germany.
FAU - Wree, Andreas
AU  - Wree A
AD  - Institute of Anatomy, Rostock University Medical Center, Rostock, Germany.
FAU - Histing, Tina
AU  - Histing T
AD  - Department of Trauma, Hand, and Reconstructive Surgery, University of Saarland, 
      Homburg/Saar, Germany.
FAU - Menger, Michael D
AU  - Menger MD
AD  - Institute for Clinical and Experimental Surgery, University of Saarland, 
      Homburg/Saar, Germany.
FAU - Muller-Hilke, Brigitte
AU  - Muller-Hilke B
AD  - Institute of Immunology, Rostock University Medical Center, Rostock, Germany.
FAU - Mittlmeier, Thomas
AU  - Mittlmeier T
AD  - Department of Trauma, Hand, and Reconstructive Surgery, Rostock University 
      Medical Center, Rostock, Germany.
FAU - Vollmar, Brigitte
AU  - Vollmar B
AD  - Institute for Experimental Surgery, Rostock University Medical Center, Rostock, 
      Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151228
PL  - England
TA  - J Bone Miner Res
JT  - Journal of bone and mineral research : the official journal of the American 
      Society for Bone and Mineral Research
JID - 8610640
SB  - IM
MH  - Animals
MH  - *Caloric Restriction
MH  - Female
MH  - Femur/*metabolism
MH  - Lumbar Vertebrae/*metabolism
MH  - Mice
MH  - Time Factors
OTO - NOTNLM
OT  - AGING
OT  - ANIMAL MODEL
OT  - BONE muCT
OT  - NUTRITION
OT  - OSTEOPOROSIS
EDAT- 2015/11/18 06:00
MHDA- 2016/12/20 06:00
CRDT- 2015/11/18 06:00
PHST- 2015/04/01 00:00 [received]
PHST- 2015/11/11 00:00 [revised]
PHST- 2015/11/13 00:00 [accepted]
PHST- 2015/11/18 06:00 [entrez]
PHST- 2015/11/18 06:00 [pubmed]
PHST- 2016/12/20 06:00 [medline]
AID - 10.1002/jbmr.2745 [doi]
PST - ppublish
SO  - J Bone Miner Res. 2016 Apr;31(4):852-63. doi: 10.1002/jbmr.2745. Epub 2015 Dec 
      28.