PMID- 26572960
OWN - NLM
STAT- MEDLINE
DCOM- 20160926
LR  - 20181202
IS  - 1791-3004 (Electronic)
IS  - 1791-2997 (Print)
IS  - 1791-2997 (Linking)
VI  - 13
IP  - 1
DP  - 2016 Jan
TI  - Irradiation alters the differentiation potential of bone marrow mesenchymal stem 
      cells.
PG  - 213-23
LID - 10.3892/mmr.2015.4539 [doi]
AB  - Bone injury following radiotherapy has been confirmed by epidemiological and 
      animal studies. However, the underlying mechanism remains to be elucidated and no 
      preventive or curative solution has been identified for this bone loss. The 
      present study aimed to investigate the irradiation‑altered osteogenesis and 
      adipogenesis of bone marrow mesenchymal stem cells (BMSCs). BMSCs were derived 
      and exposed to gamma‑irradiation at doses of 0, 0.25, 0.5, 1, 2, 5 and 10 Gy. Cell 
      viability was assessed using a 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5 diphenyl 
      tetrazolium bromide assay, and clonal expansion in vitro was detected by colony 
      forming unit assessment. The osteogenic differentiation ability was demonstrated 
      by alkaline phosphatase (ALP) activity, ALP staining and mineralization alizarin 
      red staining, and the adipogenic differentiation ability was determined using Oil 
      O red staining. The osteogenesis‑associated genes, RUNX2, ALP, osteocalcin (OCN) 
      and adipogenesis‑associated genes, PPAR‑gamma and C/EBPalpha, were detected using reverse 
      transcription‑quantitative polymerase chain reaction analyses. The protein 
      expression levels of RUNX2, ALP and PPAR‑gamma were detected using western blotting. 
      Compared with the control, significant decreases in the proliferation, ALP 
      activity and mineralization ability of the BMSCs were observed in the 
      gamma‑irradiation group, with a high level of correlation with the exposure dose. 
      However, no significant changes were observed in the area of Oil red O positive 
      staining. The mRNA levels of RUNX2, ALP and OCN were decreased (P<0.05), however, 
      no significant changes were observed in the levels of C/EBPalpha and PPAR‑gamma. The 
      protein expression levels of RUNX2 and ALP were decreased in the irradiated 
      BMSCs, however, no significant difference was observed in the protein expression 
      of PPAR‑gamma. Irradiation inhibited the osteogenic and adipogenic ability of the 
      BMSCs, and the osteogenic differentiation was decreased. The results of the 
      present study provided evidence to assist in further elucidating 
      radiotherapy‑associated side effects on the skeleton.
FAU - Wang, Yu
AU  - Wang Y
AD  - Department of Radiation Protection, Institute of Radiation Medicine, Fudan 
      University, Shanghai 200032, P.R. China.
FAU - Zhu, Guoying
AU  - Zhu G
AD  - Department of Radiation Protection, Institute of Radiation Medicine, Fudan 
      University, Shanghai 200032, P.R. China.
FAU - Wang, Jianping
AU  - Wang J
AD  - Department of Radiation Protection, Institute of Radiation Medicine, Fudan 
      University, Shanghai 200032, P.R. China.
FAU - Chen, Junxiang
AU  - Chen J
AD  - Department of Radiation Protection, Institute of Radiation Medicine, Fudan 
      University, Shanghai 200032, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151110
PL  - Greece
TA  - Mol Med Rep
JT  - Molecular medicine reports
JID - 101475259
RN  - 0 (Biomarkers)
RN  - 0 (Core Binding Factor Alpha 1 Subunit)
RN  - 0 (PPAR gamma)
RN  - 0 (RNA, Messenger)
RN  - 104982-03-8 (Osteocalcin)
RN  - EC 3.1.3.1 (Alkaline Phosphatase)
SB  - IM
MH  - Alkaline Phosphatase/metabolism
MH  - Animals
MH  - Biomarkers/metabolism
MH  - Blotting, Western
MH  - Bone Marrow Cells/*cytology/*radiation effects
MH  - Calcification, Physiologic/radiation effects
MH  - Cell Cycle/radiation effects
MH  - Cell Differentiation/*radiation effects
MH  - Cell Membrane/metabolism/radiation effects
MH  - Cell Survival/radiation effects
MH  - Cells, Cultured
MH  - Colony-Forming Units Assay
MH  - Core Binding Factor Alpha 1 Subunit/metabolism
MH  - *Gamma Rays
MH  - Gene Expression Regulation/radiation effects
MH  - Male
MH  - Mesenchymal Stem Cells/*cytology/enzymology/*radiation effects
MH  - Osteocalcin/metabolism
MH  - PPAR gamma/metabolism
MH  - RNA, Messenger/genetics/metabolism
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Staining and Labeling
PMC - PMC4686093
EDAT- 2015/11/18 06:00
MHDA- 2016/09/27 06:00
PMCR- 2015/11/10
CRDT- 2015/11/18 06:00
PHST- 2015/03/20 00:00 [received]
PHST- 2015/10/07 00:00 [accepted]
PHST- 2015/11/18 06:00 [entrez]
PHST- 2015/11/18 06:00 [pubmed]
PHST- 2016/09/27 06:00 [medline]
PHST- 2015/11/10 00:00 [pmc-release]
AID - mmr-13-01-0213 [pii]
AID - 10.3892/mmr.2015.4539 [doi]
PST - ppublish
SO  - Mol Med Rep. 2016 Jan;13(1):213-23. doi: 10.3892/mmr.2015.4539. Epub 2015 Nov 10.