PMID- 26574891
OWN - NLM
STAT- MEDLINE
DCOM- 20170302
LR  - 20220409
IS  - 1437-4331 (Electronic)
IS  - 1434-6621 (Linking)
VI  - 54
IP  - 7
DP  - 2016 Jul 1
TI  - A fast and simple method for detecting and quantifying donor-derived cell-free 
      DNA in sera of solid organ transplant recipients as a biomarker for graft 
      function.
PG  - 1147-55
LID - 10.1515/cclm-2015-0622 [doi]
AB  - BACKGROUND: Timely detection of graft rejection is an important issue in the 
      follow-up care after solid organ transplantation. Until now, biopsy has been 
      considered the "gold standard" in the diagnosis of graft rejection. However, 
      non-invasive tests such as monitoring the levels of cell-free DNA (cfDNA) as a 
      sensitive biomarker for graft integrity have attracted increasing interest. The 
      rationale of this approach is that a rejected organ will lead to a significant 
      release of donor-derived cfDNA, which can be detected in the serum of the 
      transplant recipient. METHODS: We have developed a novel quantitative real-time 
      PCR (qPCR) approach for detecting an increase of donor-derived cfDNA in the 
      recipient's serum. Common insertion/deletion (InDel) genetic polymorphisms, which 
      differ between donor and recipient, are targeted in our qPCR assay. In contrast 
      to some other strategies, no specific donor/recipient constellations such as 
      certain gender combinations or human leukocyte antigen (HLA) discrepancies are 
      required for the application of our test. RESULTS: The method was first validated 
      with serial dilutions of serum mixtures obtained from healthy blood donors and 
      then used to determine donor-derived cfDNA levels in patients' sera within the 
      first 3 days after their kidney transplantation had been performed. CONCLUSIONS: 
      Our method represents a universally applicable, simple and cost-effective tool 
      which can potentially be used to detect graft dysfunction in transplant 
      recipients.
FAU - Adamek, Martina
AU  - Adamek M
FAU - Opelz, Gerhard
AU  - Opelz G
FAU - Klein, Katrin
AU  - Klein K
FAU - Morath, Christian
AU  - Morath C
FAU - Tran, Thuong Hien
AU  - Tran TH
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PL  - Germany
TA  - Clin Chem Lab Med
JT  - Clinical chemistry and laboratory medicine
JID - 9806306
RN  - 0 (Biomarkers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Biomarkers/*blood
MH  - *Blood Donors
MH  - Case-Control Studies
MH  - DNA/*blood/genetics
MH  - Graft Rejection/blood/*diagnosis/etiology
MH  - Humans
MH  - Kidney Diseases/surgery
MH  - Kidney Transplantation/*adverse effects
MH  - Real-Time Polymerase Chain Reaction/*methods
MH  - Transplant Recipients
EDAT- 2015/11/18 06:00
MHDA- 2017/03/03 06:00
CRDT- 2015/11/18 06:00
PHST- 2015/07/01 00:00 [received]
PHST- 2015/10/17 00:00 [accepted]
PHST- 2015/11/18 06:00 [entrez]
PHST- 2015/11/18 06:00 [pubmed]
PHST- 2017/03/03 06:00 [medline]
AID - /j/cclm.ahead-of-print/cclm-2015-0622/cclm-2015-0622.xml [pii]
AID - 10.1515/cclm-2015-0622 [doi]
PST - ppublish
SO  - Clin Chem Lab Med. 2016 Jul 1;54(7):1147-55. doi: 10.1515/cclm-2015-0622.