PMID- 26576675
OWN - NLM
STAT- MEDLINE
DCOM- 20160613
LR  - 20181113
IS  - 1472-6750 (Electronic)
IS  - 1472-6750 (Linking)
VI  - 15
DP  - 2015 Nov 14
TI  - Development of a high yield expression and purification system for Domain I of 
      Beta-2-glycoprotein I for the treatment of APS.
PG  - 104
LID - 10.1186/s12896-015-0222-0 [doi]
LID - 104
AB  - BACKGROUND: In this paper we describe a novel method to achieve high yield 
      bacterial expression of a small protein domain with considerable therapeutic 
      potential; Domain I of Beta-2-glycoprotein I (beta2GPI). beta2GPI is intrinsic to the 
      pathological progression of the Antiphospholipid Syndrome (APS). Patients develop 
      autoantibodies targeting an epitope located on the N-terminal Domain I of beta2GPI 
      rendering this domain of interest as a possible therapeutic. RESULTS: This new 
      method of production of Domain I of beta2GPI has increased the production yield by 
      ~20 fold compared to previous methods in E.coli. This largely scalable, partially 
      automated method produces 50-75 mg of pure, folded, active Domain I of beta2GPI per 
      litre of expression media. CONCLUSION: The application of this method may enable 
      production of Domain I on sufficient scale to allow its use as a therapeutic.
FAU - McDonnell, Thomas
AU  - McDonnell T
AD  - Centre for Rheumatology, Division of Medicine, University College London, Rayne 
      Institute, 5 University Street, London, WC1E 6JF, UK. 
      Thomas.mcdonnell.11@ucl.ac.uk.
FAU - Pericleous, Charis
AU  - Pericleous C
AD  - Centre for Rheumatology, Division of Medicine, University College London, Rayne 
      Institute, 5 University Street, London, WC1E 6JF, UK. c.pericleous@ucl.ac.uk.
FAU - Laurine, Emmanuelle
AU  - Laurine E
AD  - PolyTherics, Babraham Research Campus, Babraham, CB22 3AT, Cambridge, UK. 
      elaurine@hotmail.com.
FAU - Tommasi, Rita
AU  - Tommasi R
AD  - PolyTherics, Babraham Research Campus, Babraham, CB22 3AT, Cambridge, UK. 
      Rita.Tommasi@gmail.com.
FAU - Garza-Garcia, Acely
AU  - Garza-Garcia A
AD  - Structural Biology, Medical Research Council National Institute for Medical 
      Research, London, UK. acely.garza@crick.ac.uk.
FAU - Giles, Ian
AU  - Giles I
AD  - Centre for Rheumatology, Division of Medicine, University College London, Rayne 
      Institute, 5 University Street, London, WC1E 6JF, UK. i.giles@ucl.ac.uk.
FAU - Ioannou, Yiannis
AU  - Ioannou Y
AD  - Centre for Rheumatology, Division of Medicine, University College London, Rayne 
      Institute, 5 University Street, London, WC1E 6JF, UK. y.ioannou@ucl.ac.uk.
AD  - Arthritis Research UK Centre for Adolescent Rheumatology, University College 
      London, London, UK. y.ioannou@ucl.ac.uk.
FAU - Rahman, Anisur
AU  - Rahman A
AD  - Centre for Rheumatology, Division of Medicine, University College London, Rayne 
      Institute, 5 University Street, London, WC1E 6JF, UK. Anisur.rahman@ucl.ac.uk.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151114
PL  - England
TA  - BMC Biotechnol
JT  - BMC biotechnology
JID - 101088663
RN  - 0 (Recombinant Fusion Proteins)
RN  - 0 (beta 2-Glycoprotein I)
SB  - IM
MH  - Adult
MH  - Antiphospholipid Syndrome/*drug therapy
MH  - Automation, Laboratory
MH  - Escherichia coli/genetics
MH  - Female
MH  - Humans
MH  - Inclusion Bodies
MH  - Male
MH  - Middle Aged
MH  - Protein Structure, Tertiary
MH  - Recombinant Fusion Proteins/genetics/*isolation & 
      purification/*metabolism/therapeutic use
MH  - beta 2-Glycoprotein I/genetics/*isolation & purification/*metabolism/therapeutic 
      use
PMC - PMC4650279
EDAT- 2015/11/19 06:00
MHDA- 2016/06/14 06:00
PMCR- 2015/11/14
CRDT- 2015/11/19 06:00
PHST- 2015/04/28 00:00 [received]
PHST- 2015/11/03 00:00 [accepted]
PHST- 2015/11/19 06:00 [entrez]
PHST- 2015/11/19 06:00 [pubmed]
PHST- 2016/06/14 06:00 [medline]
PHST- 2015/11/14 00:00 [pmc-release]
AID - 10.1186/s12896-015-0222-0 [pii]
AID - 222 [pii]
AID - 10.1186/s12896-015-0222-0 [doi]
PST - epublish
SO  - BMC Biotechnol. 2015 Nov 14;15:104. doi: 10.1186/s12896-015-0222-0.