PMID- 26578338
OWN - NLM
STAT- MEDLINE
DCOM- 20161110
LR  - 20161230
IS  - 1365-3148 (Electronic)
IS  - 0958-7578 (Linking)
VI  - 25
IP  - 6
DP  - 2015 Dec
TI  - Human leucocyte antigen alloimmunisation in repeatedly transfused thalassemic 
      Egyptian children and its relation to febrile non-haemolytic transfusion 
      reactions.
PG  - 380-4
LID - 10.1111/tme.12261 [doi]
AB  - OBJECTIVES: To document the incidence of lymphocytotoxic antibodies (LCA) in 
      chronically transfused children with beta thalassemia major and the relationship 
      between alloimmunisation and febrile non-haemolytic transfusion reactions. We 
      also compared the effect of leucoreduced-packed red blood cells (RBCs) by 
      bed-side filtration and washed RBCs in preventing FNHTRs and in inducing 
      haemoglobin rise. BACKGROUND: Alloimmunisation to human leucocytic antigens is 
      one of the common complications of transfusions, particularly in chronically 
      transfused patients as those with thalassemia major, a common disease in Northern 
      Egypt. METHODS/MATERIALS: LCA were screened for in 45 chronically transfused beta 
      thalassemia major children (group I), 20 splenectomised ones (group II) and 20 
      healthy controls (group III), using qualitative lymphocytotoxic antibody (LCA) 
      enzyme-linked immunosorbent assay (ELISA) kit. RESULTS: Nine out of 65 
      thalassemic children ( approximately 14%) were positive for LCA antibodies. Frequency of 
      transfusions and LCA positivity were significantly higher in group I than group 
      II (p = 0.036 and 0.014). There was no statistically significant difference 
      between LCA positive and negative cases regarding age of starting transfusion, 
      frequency of transfusions or FNHTRs. There was no statistically significant 
      difference between washed and filtered RBCs in reducing FNHTRs (p = 1.000) and in 
      inducing haemoglobin rise in positive LCA cases (p = 0.409). CONCLUSION: Human 
      leukocyte antigen (HLA) alloimmunisation was only 14% in the children with beta 
      thalassemia major we studied. Surprisingly FNHTRs were not more common in those 
      with HLA antibodies. Splenectomy plays a role in reducing the frequency of 
      transfusion and HLA alloimmunisation. Washed and filtered RBCs are comparable in 
      reducing FNHTRs and in inducing haemoglobin rise.
CI  - (c) 2015 British Blood Transfusion Society.
FAU - Mourad, Z
AU  - Mourad Z
AD  - Clinical Pathology Department, Faculty of Medicine, Alexandria University, 
      Alexandria, Egypt.
FAU - Hassab, H
AU  - Hassab H
AD  - Pediatric Hematology Department, Faculty of Medicine, Alexandria University, 
      Alexandria, Egypt.
FAU - Younan, D
AU  - Younan D
AD  - Clinical Pathology Department, Faculty of Medicine, Alexandria University, 
      Alexandria, Egypt.
FAU - Abdo, A
AU  - Abdo A
AD  - Clinical Pathology Department, Faculty of Medicine, Alexandria University, 
      Alexandria, Egypt.
LA  - eng
PT  - Journal Article
DEP - 20151117
PL  - England
TA  - Transfus Med
JT  - Transfusion medicine (Oxford, England)
JID - 9301182
RN  - 0 (HLA Antigens)
RN  - 0 (Isoantibodies)
SB  - IM
MH  - Child
MH  - Child, Preschool
MH  - Egypt
MH  - Erythrocyte Transfusion/*adverse effects
MH  - Female
MH  - HLA Antigens/*immunology
MH  - Humans
MH  - Infant
MH  - *Isoantibodies/blood/immunology
MH  - Male
MH  - beta-Thalassemia/blood/immunology/therapy
OTO - NOTNLM
OT  - Egyptians
OT  - FNHTRs
OT  - HLA
OT  - alloimmunisation
EDAT- 2015/11/19 06:00
MHDA- 2016/11/12 06:00
CRDT- 2015/11/19 06:00
PHST- 2014/11/15 00:00 [received]
PHST- 2015/06/06 00:00 [revised]
PHST- 2015/10/24 00:00 [accepted]
PHST- 2015/11/19 06:00 [entrez]
PHST- 2015/11/19 06:00 [pubmed]
PHST- 2016/11/12 06:00 [medline]
AID - 10.1111/tme.12261 [doi]
PST - ppublish
SO  - Transfus Med. 2015 Dec;25(6):380-4. doi: 10.1111/tme.12261. Epub 2015 Nov 17.