PMID- 26578876 OWN - NLM STAT- PubMed-not-MEDLINE DCOM- 20151120 LR - 20200930 IS - 1662-5099 (Print) IS - 1662-5099 (Electronic) IS - 1662-5099 (Linking) VI - 8 DP - 2015 TI - Dendritic targeting of short and long 3' UTR BDNF mRNA is regulated by BDNF or NT-3 and distinct sets of RNA-binding proteins. PG - 62 LID - 10.3389/fnmol.2015.00062 [doi] LID - 62 AB - Sorting of mRNAs in neuronal dendrites relies upon inducible transport mechanisms whose molecular bases are poorly understood. We investigated here the mechanism of inducible dendritic targeting of rat brain-derived neurotrophic factor (BDNF) mRNAs as a paradigmatic example. BDNF encodes multiple mRNAs with either short or long 3' UTR, both hypothesized to harbor inducible dendritic targeting signals. However, the mechanisms of sorting of the two 3' UTR isoforms are controversial. We found that dendritic localization of BDNF mRNAs with short 3' UTR was induced by depolarization and NT3 in vitro or by seizures in vivo and required CPEB-1, -2 and ELAV-2, -4. Dendritic targeting of long 3' UTR was induced by activity or BDNF and required CPEB-1 and the relief of soma-retention signals mediated by ELAV-1, -3, -4, and FXR proteins. Thus, long and short 3' UTRs, by using different sets of RNA-binding proteins provide a mechanism of selective targeting in response to different stimuli which may underlay distinct roles of BDNF variants in neuronal development and plasticity. FAU - Vicario, Annalisa AU - Vicario A AD - Department of Life Sciences, University of Trieste Trieste, Italy. FAU - Colliva, Andrea AU - Colliva A AD - Department of Life Sciences, University of Trieste Trieste, Italy. FAU - Ratti, Antonia AU - Ratti A AD - Laboratory of Neuroscience - IRCCS Istituto Auxologico Italiano Milano, Italy ; Department of Pathophysiology and Transplantation, "Dino Ferrari Center", Universita degli Studi di Milano Milano, Italy. FAU - Davidovic, Laetitia AU - Davidovic L AD - INSERM, IPMC - CNRS, UMR 7275 Valbonne, France. FAU - Baj, Gabriele AU - Baj G AD - Department of Life Sciences, University of Trieste Trieste, Italy. FAU - Gricman, Lukasz AU - Gricman L AD - Department of Life Sciences, University of Trieste Trieste, Italy. FAU - Colombrita, Claudia AU - Colombrita C AD - Laboratory of Neuroscience - IRCCS Istituto Auxologico Italiano Milano, Italy ; Department of Pathophysiology and Transplantation, "Dino Ferrari Center", Universita degli Studi di Milano Milano, Italy. FAU - Pallavicini, Alberto AU - Pallavicini A AD - Department of Life Sciences, University of Trieste Trieste, Italy. FAU - Jones, Kevin R AU - Jones KR AD - Department of Molecular Cellular and Developmental Biology, University of Colorado, Boulder CO, USA. FAU - Bardoni, Barbara AU - Bardoni B AD - INSERM, IPMC - CNRS, UMR 7275 Valbonne, France. FAU - Tongiorgi, Enrico AU - Tongiorgi E AD - Department of Life Sciences, University of Trieste Trieste, Italy. LA - eng GR - R01 EY014998/EY/NEI NIH HHS/United States PT - Journal Article DEP - 20151029 PL - Switzerland TA - Front Mol Neurosci JT - Frontiers in molecular neuroscience JID - 101477914 PMC - PMC4624863 OTO - NOTNLM OT - Fragile-X OT - RNA binding proteins OT - RNA transport OT - brain-derived neurotrophic factor OT - dendrites OT - hippocampus OT - neurotrophins EDAT- 2015/11/19 06:00 MHDA- 2015/11/19 06:01 PMCR- 2015/01/01 CRDT- 2015/11/19 06:00 PHST- 2015/04/09 00:00 [received] PHST- 2015/10/12 00:00 [accepted] PHST- 2015/11/19 06:00 [entrez] PHST- 2015/11/19 06:00 [pubmed] PHST- 2015/11/19 06:01 [medline] PHST- 2015/01/01 00:00 [pmc-release] AID - 10.3389/fnmol.2015.00062 [doi] PST - epublish SO - Front Mol Neurosci. 2015 Oct 29;8:62. doi: 10.3389/fnmol.2015.00062. eCollection 2015.