PMID- 26579122
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151120
LR  - 20181113
IS  - 1664-3224 (Print)
IS  - 1664-3224 (Electronic)
IS  - 1664-3224 (Linking)
VI  - 6
DP  - 2015
TI  - Measles Virus Epitope Presentation by HLA: Novel Insights into Epitope Selection, 
      Dominance, and Microvariation.
PG  - 546
LID - 10.3389/fimmu.2015.00546 [doi]
LID - 546
AB  - Immunity to infections with measles virus (MV) can involve vigorous human 
      leukocyte antigen (HLA) class I-restricted CD8(+) cytotoxic T cell (CTL) 
      responses. MV, albeit regarded monotypic, is known to undergo molecular evolution 
      across its RNA genome. To address which regions of the MV proteome are eligible 
      for recognition by CD8(+) CTLs and how different HLA class I loci contribute to 
      the epitope display, we interrogated the naturally processed and presented MV 
      peptidome extracted from cell lines expressing in total a broad panel of 16 
      different common HLA-A, -B, and -C molecules. The repertoire and abundance of MV 
      peptides were bona fide identified by nanoHPLC-MS/MS. -Eighty-nine MV peptides 
      were discovered and assignment to an HLA-A, -B, or -C allele, based on 
      HLA-peptide affinity prediction, was in most cases successful. Length variation 
      and presentation by multiple HLA class I molecules was common in the MV 
      peptidome. More than twice as many unique MV epitopes were found to be restricted 
      by HLA-B than by HLA-A, while MV peptides with supra-abundant expression rates 
      (>5,000 cc) were rather associated with HLA-A and HLA-C. In total, 59 regions 
      across the whole MV proteome were identified as targeted by HLA class I. Sequence 
      coverage by epitopes was highest for internal proteins transcribed from the 
      MV-P/V/C and -M genes and for hemagglutinin. At the genome level, the majority of 
      the HLA class I-selected MV epitopes represented codons having a higher 
      non-synonymous mutation rate than silent mutation rate, as established by 
      comparison of a set of 58 unique full length MV genomes. Interestingly, more 
      molecular variation was seen for the epitopes expressed at rates >/=1,000 cc. These 
      data for the first time indicate that HLA class I broadly samples the MV proteome 
      and that CTL pressure may contribute to the genomic evolution of MV.
FAU - Schellens, Ingrid M
AU  - Schellens IM
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment , Bilthoven , Netherlands ; Laboratory of Translational 
      Immunology, Department of Immunology, University Medical Center Utrecht , Utrecht 
      , Netherlands ; Department of Internal Medicine and Infectious Diseases, 
      University Medical Center Utrecht , Utrecht , Netherlands.
FAU - Meiring, Hugo D
AU  - Meiring HD
AD  - Institute for Translational Vaccinology , Bilthoven , Netherlands.
FAU - Hoof, Ilka
AU  - Hoof I
AD  - Theoretical Biology and Bioinformatics, Utrecht University , Utrecht , 
      Netherlands.
FAU - Spijkers, Sanne N
AU  - Spijkers SN
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment , Bilthoven , Netherlands ; Laboratory of Translational 
      Immunology, Department of Immunology, University Medical Center Utrecht , Utrecht 
      , Netherlands ; Department of Internal Medicine and Infectious Diseases, 
      University Medical Center Utrecht , Utrecht , Netherlands.
FAU - Poelen, Martien C M
AU  - Poelen MC
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment , Bilthoven , Netherlands.
FAU - van Gaans-van den Brink, Jacqueline A M
AU  - van Gaans-van den Brink JA
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment , Bilthoven , Netherlands.
FAU - Costa, Ana I
AU  - Costa AI
AD  - Laboratory of Translational Immunology, Department of Immunology, University 
      Medical Center Utrecht , Utrecht , Netherlands ; Department of Internal Medicine 
      and Infectious Diseases, University Medical Center Utrecht , Utrecht , 
      Netherlands.
FAU - Vennema, Harry
AU  - Vennema H
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment , Bilthoven , Netherlands.
FAU - Kesmir, Can
AU  - Kesmir C
AD  - Theoretical Biology and Bioinformatics, Utrecht University , Utrecht , 
      Netherlands.
FAU - van Baarle, Debbie
AU  - van Baarle D
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment , Bilthoven , Netherlands ; Laboratory of Translational 
      Immunology, Department of Immunology, University Medical Center Utrecht , Utrecht 
      , Netherlands ; Department of Internal Medicine and Infectious Diseases, 
      University Medical Center Utrecht , Utrecht , Netherlands.
FAU - van Els, Cecile A C M
AU  - van Els CA
AD  - Centre for Infectious Disease Control, National Institute for Public Health and 
      the Environment , Bilthoven , Netherlands.
LA  - eng
PT  - Journal Article
DEP - 20151102
PL  - Switzerland
TA  - Front Immunol
JT  - Frontiers in immunology
JID - 101560960
PMC - PMC4629467
OTO - NOTNLM
OT  - CD8 T cell epitope
OT  - HLA class I
OT  - HLA-A antigens
OT  - HLA-B antigens
OT  - HLA-C antigens
OT  - epitope mapping
OT  - immunodominance
OT  - measles virus
EDAT- 2015/11/19 06:00
MHDA- 2015/11/19 06:01
PMCR- 2015/01/01
CRDT- 2015/11/19 06:00
PHST- 2015/08/12 00:00 [received]
PHST- 2015/10/12 00:00 [accepted]
PHST- 2015/11/19 06:00 [entrez]
PHST- 2015/11/19 06:00 [pubmed]
PHST- 2015/11/19 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fimmu.2015.00546 [doi]
PST - epublish
SO  - Front Immunol. 2015 Nov 2;6:546. doi: 10.3389/fimmu.2015.00546. eCollection 2015.