PMID- 26579462
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151120
LR  - 20200930
IS  - 2211-3835 (Print)
IS  - 2211-3843 (Electronic)
IS  - 2211-3835 (Linking)
VI  - 5
IP  - 4
DP  - 2015 Jul
TI  - Neocryptotanshinone inhibits lipopolysaccharide-induced inflammation in RAW264.7 
      macrophages by suppression of NF-kappaB and iNOS signaling pathways.
PG  - 323-9
LID - 10.1016/j.apsb.2015.01.010 [doi]
AB  - Neocryptotanshinone (NCTS) is a natural product isolated from traditional Chinese 
      herb Salvia miltiorrhiza Bunge. In this study, we investigated its 
      anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated mouse macrophage 
      (RAW264.7) cells. MTT results showed that NCTS partly reversed LPS-induced 
      cytotoxicity. Real-time PCR results showed that NCTS suppressed LPS-induced mRNA 
      expression of inflammatory cytokines, including tumor necrosis factor alpha (TNFalpha), 
      interleukin-6 (IL-6) and interleukin-1beta (IL-1beta). Moreover, NCTS could decrease 
      LPS-induced nitric oxide (NO) production. Western blotting results showed that 
      NCTS could down-regulate LPS-induced expression of inducible nitric oxide 
      synthase (iNOS), p-IkappaBalpha, p-IKKbeta and p-NF-kappaB p65 without affecting 
      cyclooxygenase-2 (COX-2). In addition, NCTS inhibited LPS-induced p-NF-kappaB p65 
      nuclear translocation. In conclusion, these data demonstrated that NCTS showed 
      anti-inflammatory effect by suppression of NF-kappaB and iNOS signaling pathways.
FAU - Wu, Chuanhong
AU  - Wu C
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macau, China.
FAU - Zhao, Wenwen
AU  - Zhao W
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macau, China.
FAU - Zhang, Xuenong
AU  - Zhang X
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macau, China.
FAU - Chen, Xiuping
AU  - Chen X
AD  - State Key Laboratory of Quality Research in Chinese Medicine, Institute of 
      Chinese Medical Sciences, University of Macau, Macau, China.
LA  - eng
PT  - Journal Article
DEP - 20150407
PL  - Netherlands
TA  - Acta Pharm Sin B
JT  - Acta pharmaceutica Sinica. B
JID - 101600560
PMC - PMC4629269
OTO - NOTNLM
OT  - Inducible nitric oxide synthase
OT  - Inflammation
OT  - NF-kappaB
OT  - Neocryptotanshinone
EDAT- 2015/11/19 06:00
MHDA- 2015/11/19 06:01
PMCR- 2015/04/07
CRDT- 2015/11/19 06:00
PHST- 2014/11/10 00:00 [received]
PHST- 2015/01/27 00:00 [revised]
PHST- 2015/01/27 00:00 [accepted]
PHST- 2015/11/19 06:00 [entrez]
PHST- 2015/11/19 06:00 [pubmed]
PHST- 2015/11/19 06:01 [medline]
PHST- 2015/04/07 00:00 [pmc-release]
AID - S2211-3835(15)00025-8 [pii]
AID - 10.1016/j.apsb.2015.01.010 [doi]
PST - ppublish
SO  - Acta Pharm Sin B. 2015 Jul;5(4):323-9. doi: 10.1016/j.apsb.2015.01.010. Epub 2015 
      Apr 7.