PMID- 26580201
OWN - NLM
STAT- MEDLINE
DCOM- 20170515
LR  - 20181202
IS  - 1423-0194 (Electronic)
IS  - 0028-3835 (Print)
IS  - 0028-3835 (Linking)
VI  - 103
IP  - 6
DP  - 2016
TI  - The Distribution of Substance P and Kisspeptin in the Mediobasal Hypothalamus of 
      the Male Rhesus Monkey and a Comparison of Intravenous Administration of These 
      Peptides to Release GnRH as Reflected by LH Secretion.
PG  - 711-23
LID - 10.1159/000442420 [doi]
AB  - Substance P (SP) was recently reported to be expressed in human 
      kisspeptin/neurokinin B/dynorphin (KNDy) neurons and to enhance KNDy neuron 
      excitability in the mouse hypothalamus. We therefore examined (1) interactions of 
      SP and kisspeptin in the mediobasal hypothalamus of adult male rhesus monkeys 
      using immunofluorescence, and (2) the ability of SP to induce LH release in 
      GnRH-primed, agonadal juvenile male monkeys. SP cell bodies were observed only 
      occasionally in the arcuate nucleus (Arc), but more frequently dorsal to the Arc 
      in the region of the premammillary nucleus. Castration resulted in an increase in 
      the number of SP cell bodies in the Arc but not in the other regions. SP fibers 
      innervated the Arc, where they were found in close apposition with kisspeptin 
      perikarya in the periphery of this nucleus. Beaded SP axons projected to the 
      median eminence, where they terminated in the external layer and intermingled 
      with beaded kisspeptin axons. Colocalization of the two peptides, however, was 
      not observed. Although close apposition between SP fibers and kisspeptin neurons 
      suggest a role for SP in modulating GnRH pulse generator activity, i.v. 
      injections of SP failed to elicit release of GnRH (as reflected by LH) in the 
      juvenile monkey. Although the finding of structural interactions between SP and 
      kisspeptin neurons is consistent with the notion that this tachykinin may be 
      involved in regulating pulsatile GnRH release, the apparent absence of expression 
      of SP in KNDy neurons suggests that this peptide is unlikely to be a fundamental 
      component of the primate GnRH pulse generator.
CI  - (c) 2015 S. Karger AG, Basel.
FAU - Kalil, Bruna
AU  - Kalil B
AD  - Departamento de Fisiologia, Faculdade de Medicina de Ribeirao Preto, Universidade 
      de Sao Paulo, Ribeirao Preto, Brazil.
FAU - Ramaswamy, Suresh
AU  - Ramaswamy S
FAU - Plant, Tony M
AU  - Plant TM
LA  - eng
GR  - P30 HD008610/HD/NICHD NIH HHS/United States
GR  - R01 HD013254/HD/NICHD NIH HHS/United States
GR  - U54 HD008610/HD/NICHD NIH HHS/United States
PT  - Journal Article
DEP - 20151119
PL  - Switzerland
TA  - Neuroendocrinology
JT  - Neuroendocrinology
JID - 0035665
RN  - 0 (Kisspeptins)
RN  - 0 (Peptides)
RN  - 33507-63-0 (Substance P)
RN  - 33515-09-2 (Gonadotropin-Releasing Hormone)
RN  - 9002-67-9 (Luteinizing Hormone)
SB  - IM
MH  - Administration, Intravenous
MH  - Animals
MH  - Castration
MH  - Dose-Response Relationship, Drug
MH  - Gonadotropin-Releasing Hormone/*metabolism
MH  - *Hypothalamus, Middle/cytology/drug effects/metabolism
MH  - Kisspeptins/*metabolism
MH  - Luteinizing Hormone/*metabolism
MH  - Macaca mulatta
MH  - Male
MH  - Neurons/drug effects/metabolism
MH  - Peptides/*administration & dosage
MH  - Substance P/*metabolism
PMC - PMC4873470
MID - NIHMS745967
COIS- Statement: The authors have nothing to disclose.
EDAT- 2015/11/19 06:00
MHDA- 2017/05/16 06:00
PMCR- 2017/01/01
CRDT- 2015/11/19 06:00
PHST- 2015/08/17 00:00 [received]
PHST- 2015/11/12 00:00 [accepted]
PHST- 2015/11/19 06:00 [entrez]
PHST- 2015/11/19 06:00 [pubmed]
PHST- 2017/05/16 06:00 [medline]
PHST- 2017/01/01 00:00 [pmc-release]
AID - 000442420 [pii]
AID - 10.1159/000442420 [doi]
PST - ppublish
SO  - Neuroendocrinology. 2016;103(6):711-23. doi: 10.1159/000442420. Epub 2015 Nov 19.