PMID- 26580488
OWN - NLM
STAT- MEDLINE
DCOM- 20161213
LR  - 20161230
IS  - 1552-4930 (Electronic)
IS  - 1552-4922 (Linking)
VI  - 89
IP  - 3
DP  - 2016 Mar
TI  - Calibration of interphase fluorescence in situ hybridization cutoff by 
      mathematical models.
PG  - 239-45
LID - 10.1002/cyto.a.22797 [doi]
AB  - Fluorescence in situ hybridization (FISH) continues to play an important role in 
      clinical investigations. Laboratories may create their own cutoff, a percentage 
      of positive nuclei to determine whether a specimen is positive or negative, to 
      eliminate false positives that are created by signal overlap in most cases. In 
      some cases, it is difficult to determine the cutoff value because of differences 
      in both the area of nuclei and the number of signals. To address these problems, 
      we established two mathematical models using probability theory. To verify these 
      two models, normal disomy cells from healthy individuals were used to simulate 
      cells with different numbers of signals by hybridization with different probes. 
      We used an X/Y probe to obtain the average distance between two signals and the 
      probability of signal overlap in different nuclei area. Frequencies of all signal 
      patterns were scored and compared with theoretical frequencies, and models were 
      assessed using a goodness of fit test. We used five BCR/ABL1-positive samples, 20 
      BCR/ABL1-negative samples and two samples with ambiguous results to verify the 
      cutoff calibrated by these two models. The models were in agreement with 
      experimental results. The dynamic cutoff can classify cases in routine analysis 
      correctly, and it can also correct for influences from nuclei area and the number 
      of signals in some ambiguous cases. The probability models can be used to assess 
      the effect of signal overlap and calibrate the cutoff.
CI  - (c) 2015 International Society for Advancement of Cytometry.
FAU - Du, Qinghua
AU  - Du Q
AD  - Department of Hematology, Guangzhou First People's Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Li, Qingshan
AU  - Li Q
AD  - Department of Hematology, Guangzhou First People's Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Sun, Daochun
AU  - Sun D
AD  - School of Mathematics Science, South China Normal University, Guangzhou, China.
FAU - Chen, Xiaoyan
AU  - Chen X
AD  - Department of Hematology, Guangzhou First People's Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Yu, Bizhen
AU  - Yu B
AD  - Department of Hematology, Guangzhou First People's Hospital, Guangzhou Medical 
      University, Guangzhou, China.
FAU - Ying, Yi
AU  - Ying Y
AD  - Department of Hematology, Guangzhou First People's Hospital, Guangzhou Medical 
      University, Guangzhou, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151118
PL  - United States
TA  - Cytometry A
JT  - Cytometry. Part A : the journal of the International Society for Analytical 
      Cytology
JID - 101235694
RN  - 0 (BCR-ABL1 fusion protein, human)
RN  - 0 (DNA Probes)
RN  - EC 2.7.10.2 (Fusion Proteins, bcr-abl)
SB  - IM
MH  - Calibration/standards
MH  - Case-Control Studies
MH  - Cell Nucleus/genetics
MH  - DNA Probes/chemistry
MH  - False Positive Reactions
MH  - Fusion Proteins, bcr-abl/*genetics
MH  - Gene Expression
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*standards
MH  - Interphase/*genetics
MH  - Leukemia, Myelogenous, Chronic, BCR-ABL Positive/*diagnosis/genetics/pathology
MH  - Male
MH  - *Models, Statistical
MH  - Myeloid Cells/*metabolism/pathology
OTO - NOTNLM
OT  - cell nucleus/genetics
OT  - fluorescence in situ hybridization
OT  - mathematical model
OT  - reference standards
OT  - spot counting
EDAT- 2015/11/19 06:00
MHDA- 2016/12/15 06:00
CRDT- 2015/11/19 06:00
PHST- 2014/09/29 00:00 [received]
PHST- 2015/07/05 00:00 [revised]
PHST- 2015/10/23 00:00 [accepted]
PHST- 2015/11/19 06:00 [entrez]
PHST- 2015/11/19 06:00 [pubmed]
PHST- 2016/12/15 06:00 [medline]
AID - 10.1002/cyto.a.22797 [doi]
PST - ppublish
SO  - Cytometry A. 2016 Mar;89(3):239-45. doi: 10.1002/cyto.a.22797. Epub 2015 Nov 18.