PMID- 26581032
OWN - NLM
STAT- MEDLINE
DCOM- 20170915
LR  - 20180816
IS  - 1097-4644 (Electronic)
IS  - 0730-2312 (Linking)
VI  - 117
IP  - 7
DP  - 2016 Jul
TI  - Distinction Between Cell Proliferation and Apoptosis Signals Regulated by 
      Brain-Derived Neurotrophic Factor in Human Periodontal Ligament Cells and 
      Gingival Epithelial Cells.
PG  - 1543-55
LID - 10.1002/jcb.25446 [doi]
AB  - Previously, we reported that brain-derived neurotrophic factor (BDNF) enhances 
      periodontal tissue regeneration by inducing periodontal ligament cell 
      proliferation in vivo. In addition, the down growth of gingival epithelial cells, 
      which comprises a major obstacle to the regeneration, was not observed. However, 
      the underlying molecular mechanism is still unclear. Therefore, this study aimed 
      to investigate the effect of BDNF on cell proliferation and apoptosis in human 
      periodontal ligament (HPL) cells and human gingival epithelial cells (OBA9 cells) 
      and to explore the molecular mechanism in vitro. HPL cells dominantly expressed a 
      BDNF receptor, TrkB, and BDNF increased cell proliferation and ERK 
      phosphorylation. However, its proliferative effect was diminished by a MEK1/2 
      inhibitor (U0126) and TrkB siRNA transfection. Otherwise, OBA9 cells showed a 
      higher expression level of p75, which is a pan-neurotrophin receptor, than that 
      of HPL cells. BDNF facilitated not cell proliferation but cell apoptosis and JNK 
      phosphorylation in OBA9 cells. A JNK inhibitor (SP600125) and p75 siRNA 
      transfection attenuated the BDNF-induced cell apoptosis. Moreover, OBA9 cells 
      pretreated with SP600125 or p75 siRNA showed cell proliferation by BDNF 
      stimulation, though it was reduced by U0126 and TrkB siRNA. Interestingly, 
      overexpression of p75 in HPL cells upregulated cell apoptosis and JNK 
      phosphorylation by BDNF treatment. These results indicated that TrkB-ERK 
      signaling regulates BDNF-induced cell proliferation, whereas p75-JNK signaling 
      plays roles in cell apoptotic and cytostatic effect of BDNF. Overall, BDNF 
      activates periodontal ligament cells proliferation and inhibits the gingival 
      epithelial cells growth via the distinct pathway. J. Cell. Biochem. 117: 
      1543-1555, 2016. (c) 2015 Wiley Periodicals, Inc.
CI  - (c) 2015 Wiley Periodicals, Inc.
FAU - Kashiwai, Kei
AU  - Kashiwai K
AD  - Department of Periodontal Medicine, Applied Life Sciences, Institute of 
      Biomedical & Health Sciences, Graduate School of Biomedical & Health Sciences, 
      Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.
FAU - Kajiya, Mikihito
AU  - Kajiya M
AD  - Department of Periodontal Medicine, Applied Life Sciences, Institute of 
      Biomedical & Health Sciences, Graduate School of Biomedical & Health Sciences, 
      Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.
FAU - Matsuda, Shinji
AU  - Matsuda S
AD  - Department of Periodontal Medicine, Applied Life Sciences, Institute of 
      Biomedical & Health Sciences, Graduate School of Biomedical & Health Sciences, 
      Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.
FAU - Ouhara, Kazuhisa
AU  - Ouhara K
AD  - Department of Periodontal Medicine, Applied Life Sciences, Institute of 
      Biomedical & Health Sciences, Graduate School of Biomedical & Health Sciences, 
      Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.
FAU - Takeda, Katsuhiro
AU  - Takeda K
AD  - Department of Periodontal Medicine, Applied Life Sciences, Institute of 
      Biomedical & Health Sciences, Graduate School of Biomedical & Health Sciences, 
      Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.
FAU - Takata, Takashi
AU  - Takata T
AD  - Department of Oral and Maxillofacial Pathology, Basic Life Sciences, Institute of 
      Biomedical and Health Science, Graduate School of Biomedical & Health Sciences, 
      Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.
FAU - Kitagawa, Masae
AU  - Kitagawa M
AD  - Center of Oral Clinical Examination, Hiroshima University Hospital, 1-2-3, 
      Kasumi, Minami-ku, Hiroshima, Japan.
FAU - Fujita, Tsuyoshi
AU  - Fujita T
AD  - Department of Periodontal Medicine, Applied Life Sciences, Institute of 
      Biomedical & Health Sciences, Graduate School of Biomedical & Health Sciences, 
      Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.
FAU - Shiba, Hideki
AU  - Shiba H
AD  - Department of Periodontal Medicine, Applied Life Sciences, Institute of 
      Biomedical & Health Sciences, Graduate School of Biomedical & Health Sciences, 
      Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.
FAU - Kurihara, Hidemi
AU  - Kurihara H
AD  - Department of Periodontal Medicine, Applied Life Sciences, Institute of 
      Biomedical & Health Sciences, Graduate School of Biomedical & Health Sciences, 
      Hiroshima University, 1-2-3, Kasumi, Minami-ku, Hiroshima, Japan.
LA  - eng
PT  - Journal Article
DEP - 20151208
PL  - United States
TA  - J Cell Biochem
JT  - Journal of cellular biochemistry
JID - 8205768
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Apoptosis/*drug effects
MH  - Brain-Derived Neurotrophic Factor/*pharmacology
MH  - Cell Line, Transformed
MH  - Cell Proliferation/*drug effects
MH  - Epithelial Cells/cytology/*metabolism
MH  - Gingiva/cytology/*metabolism
MH  - Humans
MH  - MAP Kinase Signaling System/*drug effects
MH  - Periodontal Ligament/cytology/*metabolism
OTO - NOTNLM
OT  - BDNF
OT  - ERK
OT  - JNK
OT  - TrkB
OT  - p75
EDAT- 2015/11/19 06:00
MHDA- 2017/09/16 06:00
CRDT- 2015/11/19 06:00
PHST- 2015/11/16 00:00 [received]
PHST- 2015/11/17 00:00 [accepted]
PHST- 2015/11/19 06:00 [entrez]
PHST- 2015/11/19 06:00 [pubmed]
PHST- 2017/09/16 06:00 [medline]
AID - 10.1002/jcb.25446 [doi]
PST - ppublish
SO  - J Cell Biochem. 2016 Jul;117(7):1543-55. doi: 10.1002/jcb.25446. Epub 2015 Dec 8.