PMID- 26581169
OWN - NLM
STAT- MEDLINE
DCOM- 20160914
LR  - 20240324
IS  - 2045-2322 (Electronic)
IS  - 2045-2322 (Linking)
VI  - 5
DP  - 2015 Nov 19
TI  - Cellular miR-130b inhibits replication of porcine reproductive and respiratory 
      syndrome virus in vitro and in vivo.
PG  - 17010
LID - 10.1038/srep17010 [doi]
LID - 17010
AB  - MicroRNAs (miRNAs) can impact viral infections by binding to sequences with 
      partial complementarity on viral RNA transcripts, usually resulting in the 
      repression of virus replication. In the present study, we identified a potential 
      binding site for miR-130 in the 5' untranslated region (bps 155-162) of the 
      porcine reproductive and respiratory syndrome virus (PRRSV) genome. We found that 
      the delivery of multiple miR-130 family mimics, especially miR-130b, resulted in 
      inhibition of PRRSV replication in vitro. miR-130 was effective in inhibiting the 
      replication of multiple type 2 PRRSV strains, but not against vSHE, a classical 
      type 1 strain. miR-130 over-expression did not induce IFN-alpha or TNF-alpha expression 
      in either uninfected or PRRSV-infected porcine alveolar macrophages. Results from 
      luciferase reporter assays indicated that miR-130 directly targeted the PRRSV 5' 
      UTR. Intranasal inoculation of piglets with miR-130b exhibited antiviral activity 
      in vivo and partially protected piglets from an otherwise lethal challenge with 
      HP-PRRSV strain vJX143. Overall, these results demonstrate the importance of the 
      miR-130 family in modulating PRRSV replication and also provide a scientific 
      basis for using cellular miRNAs in anti-PRRSV therapies.
FAU - Li, Liwei
AU  - Li L
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
FAU - Gao, Fei
AU  - Gao F
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, 225009, P.R. China.
FAU - Jiang, Yifeng
AU  - Jiang Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, 225009, P.R. China.
FAU - Yu, Lingxue
AU  - Yu L
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
FAU - Zhou, Yanjun
AU  - Zhou Y
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
FAU - Zheng, Hao
AU  - Zheng H
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
FAU - Tong, Wu
AU  - Tong W
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
FAU - Yang, Shen
AU  - Yang S
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
FAU - Xia, Tianqi
AU  - Xia T
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
FAU - Qu, Zehui
AU  - Qu Z
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
FAU - Tong, Guangzhi
AU  - Tong G
AD  - Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 
      Shanghai 200241, P.R. China.
AD  - Jiangsu Co-innovation Center for Prevention and Control of Important Animal 
      Infectious Diseases and Zoonoses, Yangzhou, 225009, P.R. China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151119
PL  - England
TA  - Sci Rep
JT  - Scientific reports
JID - 101563288
RN  - 0 (5' Untranslated Regions)
RN  - 0 (Antiviral Agents)
RN  - 0 (Interferon-alpha)
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
SB  - IM
MH  - 5' Untranslated Regions/genetics
MH  - Administration, Intranasal
MH  - Animals
MH  - Antiviral Agents/pharmacology
MH  - Base Sequence
MH  - Gene Expression Regulation/drug effects
MH  - Genome
MH  - Interferon-alpha/genetics/metabolism
MH  - MicroRNAs/administration & dosage/*metabolism
MH  - Molecular Sequence Data
MH  - Porcine respiratory and reproductive syndrome virus/drug effects/*growth & 
      development
MH  - RNA, Messenger/genetics/metabolism
MH  - Sus scrofa
MH  - Tumor Necrosis Factor-alpha/genetics/metabolism
MH  - *Virus Replication/drug effects
PMC - PMC4652204
EDAT- 2015/11/20 06:00
MHDA- 2016/09/15 06:00
PMCR- 2015/11/19
CRDT- 2015/11/20 06:00
PHST- 2015/05/18 00:00 [received]
PHST- 2015/10/20 00:00 [accepted]
PHST- 2015/11/20 06:00 [entrez]
PHST- 2015/11/20 06:00 [pubmed]
PHST- 2016/09/15 06:00 [medline]
PHST- 2015/11/19 00:00 [pmc-release]
AID - srep17010 [pii]
AID - 10.1038/srep17010 [doi]
PST - epublish
SO  - Sci Rep. 2015 Nov 19;5:17010. doi: 10.1038/srep17010.