PMID- 26581328
OWN - NLM
STAT- MEDLINE
DCOM- 20160408
LR  - 20151215
IS  - 1879-0631 (Electronic)
IS  - 0024-3205 (Linking)
VI  - 143
DP  - 2015 Dec 15
TI  - Chemokine profiles of human visceral adipocytes from cryopreserved preadipocytes: 
      Neutrophil activation and induction of nuclear factor-kappa B repressing factor.
PG  - 225-30
LID - S0024-3205(15)30073-4 [pii]
LID - 10.1016/j.lfs.2015.11.010 [doi]
AB  - AIMS: In obesity, infiltration of adipose tissue by proinflammatory immune cells 
      causes chronic low-grade inflammation. We investigated the chemokine profiles of 
      human visceral adipocytes by the reverse transcription polymerase chain reaction 
      and the effect of human neutrophil elastase (HNE) on monocyte chemoattractant 
      protein-1 (MCP-1) mRNA and protein levels. MAIN METHODS: Human adipocytes were 
      obtained from cryopreserved omental preadipocytes of subjects with a body mass 
      index (BMI) <30kg/m(2) or >30kg/m(2) and were cultured to assess chemokine 
      production. KEY FINDINGS: Chemokine responses associated with obesity-related 
      inflammation were well preserved in cultured human adipocytes derived from 
      cryopreserved preadipocytes. Visceral adipocytes from subjects with a BMI 
      >30kg/m(2) expressed mRNA for MCP-1, regulated on activation, normal T cell 
      expressed and secreted (RANTES), epithelial cell-derived neutrophil-activating 
      peptide-78 (ENA-78), interleukin-8 (IL-8), lymphotactin-beta, and fractalkine. 
      Although visceral adipocytes from subjects with a BMI <30kg/m(2) also expressed 
      MCP-1, RANTES, ENA-78, and IL-8 mRNA, neither lymphotactin-beta nor fraktalkine mRNA 
      was detected. Interestingly, expression of MCP-1 mRNA was decreased significantly 
      after exposure to HNE (85x10(3)muM/L), suggesting the induction of nuclear 
      factor-kappa B repressing factor. SIGNIFICANCE: Adipocytes from subjects with a 
      BMI >30kg/m(2) or <30kg/m(2) have different chemokine profiles. Only adipocytes 
      from subjects with a BMI >30kg/m(2) express lymphotactin-beta and fractalkine mRNA. 
      Differential chemokine profiles of visceral adipocytes contribute to infiltration 
      of adipose tissue by adaptive immune cells. Neutrophil activation is involved in 
      induction of nuclear factor-kappa B repressing factor, resulting in regulation of 
      immune cell trafficking.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Yamaguchi, Rui
AU  - Yamaguchi R
AD  - Graduate School of Medical Science, Kumamoto Health Science University, Kumamoto, 
      Japan; Graduate School of Medical Sciences, Kumamoto University Medical School, 
      Kumamoto, Japan.
FAU - Yamamoto, Takatoshi
AU  - Yamamoto T
AD  - Graduate School of Medical Science, Kumamoto Health Science University, Kumamoto, 
      Japan.
FAU - Sakamoto, Arisa
AU  - Sakamoto A
AD  - Graduate School of Medical Science, Kumamoto Health Science University, Kumamoto, 
      Japan.
FAU - Ishimaru, Yasuji
AU  - Ishimaru Y
AD  - Graduate School of Medical Science, Kumamoto Health Science University, Kumamoto, 
      Japan.
FAU - Narahara, Shinji
AU  - Narahara S
AD  - Graduate School of Medical Science, Kumamoto Health Science University, Kumamoto, 
      Japan.
FAU - Sugiuchi, Hiroyuki
AU  - Sugiuchi H
AD  - Graduate School of Medical Science, Kumamoto Health Science University, Kumamoto, 
      Japan.
FAU - Yamaguchi, Yasuo
AU  - Yamaguchi Y
AD  - Graduate School of Medical Science, Kumamoto Health Science University, Kumamoto, 
      Japan. Electronic address: yamaguti@kumamoto-hsu.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151112
PL  - Netherlands
TA  - Life Sci
JT  - Life sciences
JID - 0375521
RN  - 0 (Chemokines)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - Adipocytes/*metabolism
MH  - *Body Mass Index
MH  - Cell Line
MH  - Cells, Cultured
MH  - Chemokines/*metabolism
MH  - Cryopreservation
MH  - Humans
MH  - Intra-Abdominal Fat/cytology/*metabolism
MH  - NF-kappa B/*biosynthesis
MH  - Neutrophil Activation/*physiology
OTO - NOTNLM
OT  - Adaptive immunity
OT  - Human visceral adipocytes
OT  - Neutrophil elastase
EDAT- 2015/11/20 06:00
MHDA- 2016/04/09 06:00
CRDT- 2015/11/20 06:00
PHST- 2015/04/13 00:00 [received]
PHST- 2015/07/18 00:00 [revised]
PHST- 2015/11/11 00:00 [accepted]
PHST- 2015/11/20 06:00 [entrez]
PHST- 2015/11/20 06:00 [pubmed]
PHST- 2016/04/09 06:00 [medline]
AID - S0024-3205(15)30073-4 [pii]
AID - 10.1016/j.lfs.2015.11.010 [doi]
PST - ppublish
SO  - Life Sci. 2015 Dec 15;143:225-30. doi: 10.1016/j.lfs.2015.11.010. Epub 2015 Nov 
      12.