PMID- 26581717
OWN - NLM
STAT- MEDLINE
DCOM- 20190327
LR  - 20190327
IS  - 1472-6831 (Electronic)
IS  - 1472-6831 (Linking)
VI  - 15
DP  - 2015 Nov 18
TI  - Periodontal CD14 mRNA expression is downregulated in patients with chronic 
      periodontitis and type 2 diabetes.
PG  - 145
LID - 10.1186/s12903-015-0118-3 [doi]
LID - 145
AB  - BACKGROUND: Patients with type 2 diabetes mellitus (T2DM) have increased severity 
      of periodontitis. Toll-like receptor (TLR)4, its co-receptors CD14 and MD-2, and 
      adaptor MyD88 play pivotal roles in lipopolysaccharide (LPS)-triggered tissue 
      inflammation and periodontitis. This study investigated the effects of T2DM and 
      periodontitis on TLR4, CD14, MD-2 and MyD88 mRNA expression in surgically removed 
      periodontal tissues. METHODS: Periodontal tissue specimens were collected from 14 
      patients without periodontitis and T2DM (Group 1), 15 patients with periodontitis 
      alone (Group 2), and 7 patients with both periodontitis and T2DM (Group 3). The 
      mRNA of TLR4, CD14, MD-2 and MyD88 was quantified using real-time PCR and 
      compared between the groups. RESULTS: Statistical analysis showed that 
      periodontal expression of CD14 mRNA was significantly reduced across Groups 1, 2 
      and 3 (p = 0.02) whereas the mRNA expression of TLR4, MD-2 and MyD88 was not 
      significantly different among the groups. Furthermore, when patients in Groups 1 
      and 2 were combined (n = 22), the CD14 mRNA expression was significantly lower 
      than that in patients of Group 1 (p = 0.04). CONCLUSIONS: CD14 mRNA expression 
      was downregulated across patients with neither periodontitis nor T2DM, patients 
      with periodontitis alone and patients with both diseases, suggesting that CD14 
      mRNA expression is associated with a favorable host response or subjected to a 
      negative feedback regulation.
FAU - Hedgpeth, Dustin C
AU  - Hedgpeth DC
AD  - Department of Stomatology, James B. Edwards College of Dental Medicine, Medical 
      University of South Carolina, Charleston, SC, 29425, USA. 
      dustin.hedgpeth@gmail.com.
FAU - Zhang, Xiaoming
AU  - Zhang X
AD  - Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, 
      College of Medicine, Medical University of South Carolina, 114 Doughty Street, 
      Charleston, SC, 29425, USA. 2226710963@qq.com.
FAU - Jin, Junfei
AU  - Jin J
AD  - Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, 
      College of Medicine, Medical University of South Carolina, 114 Doughty Street, 
      Charleston, SC, 29425, USA. jinj100@yahoo.com.
AD  - Laboratory of Hepatobiliary and Pancreatic Surgery, Affiliated Hospital of Guilin 
      Medical University, Guilin, 541001, Guangxi, People's Republic of China. 
      jinj100@yahoo.com.
FAU - Leite, Renata S
AU  - Leite RS
AD  - Department of Stomatology, James B. Edwards College of Dental Medicine, Medical 
      University of South Carolina, Charleston, SC, 29425, USA. leiter@musc.edu.
AD  - Center for Oral Health Research, James B. Edwards College of Dental Medicine, 
      Medical University of South Carolina, Charleston, SC, 29425, USA. 
      leiter@musc.edu.
FAU - Krayer, Joe W
AU  - Krayer JW
AD  - Department of Stomatology, James B. Edwards College of Dental Medicine, Medical 
      University of South Carolina, Charleston, SC, 29425, USA. krayer@musc.edu.
FAU - Huang, Yan
AU  - Huang Y
AD  - Division of Endocrinology, Diabetes and Medical Genetics, Department of Medicine, 
      College of Medicine, Medical University of South Carolina, 114 Doughty Street, 
      Charleston, SC, 29425, USA. huangyan@musc.edu.
AD  - Ralph H. Johnson VA Medical Center, 114 Doughty Street, Charleston, SC, 29401, 
      USA. huangyan@musc.edu.
LA  - eng
GR  - I01 BX000854/BX/BLRD VA/United States
GR  - R01 DE016353/DE/NIDCR NIH HHS/United States
GR  - DE016353/DE/NIDCR NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20151118
PL  - England
TA  - BMC Oral Health
JT  - BMC oral health
JID - 101088684
RN  - 0 (LY96 protein, human)
RN  - 0 (Lipopolysaccharide Receptors)
RN  - 0 (Lymphocyte Antigen 96)
RN  - 0 (MYD88 protein, human)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - 0 (RNA, Messenger)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - *Chronic Periodontitis/complications/metabolism
MH  - *Diabetes Mellitus, Type 2/complications/metabolism
MH  - Down-Regulation
MH  - Humans
MH  - *Lipopolysaccharide Receptors/metabolism
MH  - Lymphocyte Antigen 96/metabolism
MH  - Myeloid Differentiation Factor 88/metabolism
MH  - RNA, Messenger/metabolism
MH  - Toll-Like Receptor 4/metabolism
PMC - PMC4652420
EDAT- 2015/11/20 06:00
MHDA- 2019/03/28 06:00
PMCR- 2015/11/18
CRDT- 2015/11/20 06:00
PHST- 2015/05/17 00:00 [received]
PHST- 2015/10/15 00:00 [accepted]
PHST- 2015/11/20 06:00 [entrez]
PHST- 2015/11/20 06:00 [pubmed]
PHST- 2019/03/28 06:00 [medline]
PHST- 2015/11/18 00:00 [pmc-release]
AID - 10.1186/s12903-015-0118-3 [pii]
AID - 118 [pii]
AID - 10.1186/s12903-015-0118-3 [doi]
PST - epublish
SO  - BMC Oral Health. 2015 Nov 18;15:145. doi: 10.1186/s12903-015-0118-3.