PMID- 26581909
OWN - NLM
STAT- MEDLINE
DCOM- 20170203
LR  - 20181113
IS  - 1423-0380 (Electronic)
IS  - 1010-4283 (Linking)
VI  - 37
IP  - 5
DP  - 2016 May
TI  - MicroRNA-15a-5p suppresses cancer proliferation and division in human 
      hepatocellular carcinoma by targeting BDNF.
PG  - 5821-8
LID - 10.1007/s13277-015-4427-6 [doi]
AB  - We examined the expression pattern and functional roles of microRNA 15a-5p 
      (miR-15a-5p) in human hepatocellular carcinoma (HCC). Possible miR-15a-5p 
      aberrant expression in HCC cell lines or clinical HCC specimens was examined by 
      quantitative real-time PCR (qRT-PCR). In HCC HepG2 and SNU-182 cells, miR-15a-5p 
      was ectopically overexpressed by lentiviral transduction. Its effect on HCC 
      proliferation, cancer division, and in vivo tumor growth were examined by 
      3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, cell 
      cycle assay, and tumorigenicity assay, respectively. The targeting of miR-15a-5p 
      on its downstream gene, brain-derived neurotrophic factor (BDNF), was examined by 
      dual-luciferase assay, qRT-PCR, and Western blot, respectively. BDNF was then 
      overexpressed in HepG2 and SNU-182 cells to evaluate its selective effect on 
      miR-15a-5p in HCC modulation. MiR-15a-5p is aberrantly downregulated in in vitro 
      HCC cell lines and in vivo HCC clinical specimens. Ectopic overexpression of 
      miR-15a-5p suppressed cancer proliferation, induced cell cycle arrest in HepG2 or 
      SNU-182 cells in vitro, and inhibited HCC tumor growth in vivo. MiR-15a-5p 
      selectively and negatively regulated BDNF at both gene and protein levels in HCC 
      cells. Forced overexpression of BDNF effectively reversed the tumor suppressive 
      functions of miR-15a-5p on HCC proliferation and cell division in vitro. Our 
      study demonstrated that miR-15a-5p is a tumor suppressor in HCC and its 
      regulation is through BDNF in HCC.
FAU - Long, Jianting
AU  - Long J
AD  - Department of Medicinal Oncology, The First Affiliated Hospital, Sun Yat-sen 
      University, Guangzhou, 510080, China.
FAU - Jiang, Chunlin
AU  - Jiang C
AD  - Department of Hepatobiliary Surgery, Division of Interventional Ultrasound, The 
      First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China.
FAU - Liu, Baoxian
AU  - Liu B
AD  - Department of Medical Ultrasonics, Institute of Diagnostic and Interventional 
      Ultrasound, The First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 
      510080, China.
FAU - Fang, Shi
AU  - Fang S
AD  - Department of Hepatobiliary Surgery, Division of Interventional Ultrasound, The 
      First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. 
      fangshi@mail.sysu.edu.cn.
AD  - Department of Clinic Nutrition, The First Affiliated Hospital of Sun Yat-sen 
      University, 58 2nd Zhongshan Rd, Guangzhou, 510080, China. 
      fangshi@mail.sysu.edu.cn.
FAU - Kuang, Ming
AU  - Kuang M
AD  - Department of Hepatobiliary Surgery, Division of Interventional Ultrasound, The 
      First Affiliated Hospital, Sun Yat-sen University, Guangzhou, 510080, China. 
      kuangm@mail.sysu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20151118
PL  - Netherlands
TA  - Tumour Biol
JT  - Tumour biology : the journal of the International Society for Oncodevelopmental 
      Biology and Medicine
JID - 8409922
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (MIRN15 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Neoplasm Proteins)
RN  - 0 (RNA, Neoplasm)
RN  - 0 (Recombinant Fusion Proteins)
RN  - 7171WSG8A2 (BDNF protein, human)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/genetics/*physiology
MH  - Carcinoma, Hepatocellular/genetics/*pathology
MH  - Cell Division
MH  - Cell Line, Tumor
MH  - Down-Regulation
MH  - Female
MH  - Gene Expression Regulation, Neoplastic
MH  - Genes, Tumor Suppressor
MH  - Genetic Vectors
MH  - Hep G2 Cells/transplantation
MH  - Humans
MH  - Lentivirus
MH  - Liver Neoplasms/genetics/*pathology
MH  - Mice
MH  - Mice, Nude
MH  - MicroRNAs/*genetics
MH  - Neoplasm Proteins/genetics/*physiology
MH  - RNA, Neoplasm/*genetics
MH  - Recombinant Fusion Proteins/metabolism
OTO - NOTNLM
OT  - BDNF
OT  - Cancer proliferation
OT  - Cell cycle
OT  - Hepatocellular carcinoma
OT  - miR-15a-5p
EDAT- 2015/11/20 06:00
MHDA- 2017/02/06 06:00
CRDT- 2015/11/20 06:00
PHST- 2015/09/24 00:00 [received]
PHST- 2015/11/10 00:00 [accepted]
PHST- 2015/11/20 06:00 [entrez]
PHST- 2015/11/20 06:00 [pubmed]
PHST- 2017/02/06 06:00 [medline]
AID - 10.1007/s13277-015-4427-6 [pii]
AID - 10.1007/s13277-015-4427-6 [doi]
PST - ppublish
SO  - Tumour Biol. 2016 May;37(5):5821-8. doi: 10.1007/s13277-015-4427-6. Epub 2015 Nov 
      18.