PMID- 26582639
OWN - NLM
STAT- MEDLINE
DCOM- 20160502
LR  - 20200930
IS  - 1522-1490 (Electronic)
IS  - 0363-6119 (Print)
IS  - 0363-6119 (Linking)
VI  - 310
IP  - 2
DP  - 2016 Jan 15
TI  - Acclimation and acute temperature effects on population differences in oxidative 
      phosphorylation.
PG  - R185-96
LID - 10.1152/ajpregu.00421.2015 [doi]
AB  - Temperature changes affect metabolism on acute, acclamatory, and evolutionary 
      time scales. To better understand temperature's affect on metabolism at these 
      different time scales, we quantified cardiac oxidative phosphorylation (OxPhos) 
      in three Fundulus taxa acclimated to 12 and 28 degrees C and measured at three acute 
      temperatures (12, 20, and 28 degrees C). The Fundulus taxa (northern Maine and southern 
      Georgia F. heteroclitus, and a sister taxa, F. grandis) were used to identify 
      evolved changes in OxPhos. Cardiac OxPhos metabolism was quantified by measuring 
      six traits: state 3 (ADP and substrate-dependent mitochondrial respiration); E 
      state (uncoupled mitochondrial activity); complex I, II, and IV activities; and 
      LEAK ratio. Acute temperature affected all OxPhos traits. Acclimation only 
      significantly affected state 3 and LEAK ratio. Populations were significantly 
      different for state 3. In addition to direct effects, there were significant 
      interactions between acclimation and population for complex I and between 
      population and acute temperature for state 3. Further analyses suggest that 
      acclimation alters the acute temperature response for state 3, E state, and 
      complexes I and II: at the low acclimation temperature, the acute response was 
      dampened at low assay temperatures, and at the high acclimation temperature, the 
      acute response was dampened at high assay temperatures. Closer examination of the 
      data also suggests that differences in state 3 respiration and complex I activity 
      between populations were greatest between fish acclimated to low temperatures 
      when assayed at high temperatures, suggesting that differences between the 
      populations become more apparent at the edges of their thermal range.
CI  - Copyright (c) 2016 the American Physiological Society.
FAU - Baris, Tara Z
AU  - Baris TZ
AD  - Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Sciences, 
      University of Miami, Miami, Florida.
FAU - Crawford, Douglas L
AU  - Crawford DL
AD  - Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Sciences, 
      University of Miami, Miami, Florida dcrawford@rsmas.miami.edu.
FAU - Oleksiak, Marjorie F
AU  - Oleksiak MF
AD  - Marine Biology and Ecology, Rosenstiel School of Marine and Atmospheric Sciences, 
      University of Miami, Miami, Florida.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, U.S. Gov't, Non-P.H.S.
DEP - 20151118
PL  - United States
TA  - Am J Physiol Regul Integr Comp Physiol
JT  - American journal of physiology. Regulatory, integrative and comparative 
      physiology
JID - 100901230
RN  - 0 (Electron Transport Chain Complex Proteins)
RN  - 0 (Fish Proteins)
RN  - 61D2G4IYVH (Adenosine Diphosphate)
SB  - IM
MH  - *Acclimatization
MH  - Adenosine Diphosphate/metabolism
MH  - Animals
MH  - Cell Respiration
MH  - Electron Transport Chain Complex Proteins/metabolism
MH  - Fish Proteins/metabolism
MH  - Fundulidae/classification/*metabolism
MH  - Mitochondria/metabolism
MH  - Myocardium/*metabolism
MH  - *Oxidative Phosphorylation
MH  - Species Specificity
MH  - *Temperature
MH  - Time Factors
PMC - PMC4796645
OTO - NOTNLM
OT  - enzyme complexes
OT  - fundulus
OT  - mitochondria
OT  - oxidative phosphorylation
OT  - state 3
EDAT- 2015/11/20 06:00
MHDA- 2016/05/03 06:00
PMCR- 2017/01/15
CRDT- 2015/11/20 06:00
PHST- 2015/09/30 00:00 [received]
PHST- 2015/11/12 00:00 [accepted]
PHST- 2015/11/20 06:00 [entrez]
PHST- 2015/11/20 06:00 [pubmed]
PHST- 2016/05/03 06:00 [medline]
PHST- 2017/01/15 00:00 [pmc-release]
AID - ajpregu.00421.2015 [pii]
AID - R-00421-2015 [pii]
AID - 10.1152/ajpregu.00421.2015 [doi]
PST - ppublish
SO  - Am J Physiol Regul Integr Comp Physiol. 2016 Jan 15;310(2):R185-96. doi: 
      10.1152/ajpregu.00421.2015. Epub 2015 Nov 18.