PMID- 26584215
OWN - NLM
STAT- MEDLINE
DCOM- 20160425
LR  - 20151215
IS  - 1096-0341 (Electronic)
IS  - 0042-6822 (Linking)
VI  - 487
DP  - 2016 Jan
TI  - Hemopoietic cell kinase (Hck) and p21-activated kinase 2 (PAK2) are involved in 
      the down-regulation of CD1a lipid antigen presentation by HIV-1 Nef in dendritic 
      cells.
PG  - 285-95
LID - S0042-6822(15)00451-1 [pii]
LID - 10.1016/j.virol.2015.10.023 [doi]
AB  - Dendritic cells (DCs) play a major role in in vivo pathogenesis of HIV-1 
      infection. Therefore, DCs may provide a promising strategy to control and 
      eventually overcome the fatal infection. Especially, immature DCs express all 
      CD1s, the non-MHC lipid antigen -presenting molecules, and HIV-1 Nef 
      down-regulates CD1 expression besides MHC. Moreover, CD1d-restricted CD4(+) NKT 
      cells are infected by HIV-1, reducing the number of these cells in HIV-1-infected 
      individuals. To understand the exact role of DCs and CD1-mediated immune response 
      during HIV-1 infection, Nef down-regulation of CD1a-restricted lipid/glycolipid 
      Ag presentation in iDCs was analyzed. We demonstrated the involvement of the 
      association of Nef with hemopoietic cell kinase (Hck) and p21-activated kinase 2 
      (PAK2), and that Hck, which is expressed strongly in iDCs, augmented this mutual 
      interaction. Hck might be another therapeutic target to preserve the function of 
      HIV-1 infected DCs, which are potential reservoirs of HIV-1 even after 
      antiretroviral therapy.
CI  - Copyright (c) 2015 Elsevier Inc. All rights reserved.
FAU - Shinya, Eiji
AU  - Shinya E
AD  - Department of Microbiology and Immunology, Nippon Medical School, 1-1-5 Sendagi, 
      Bunkyo city, Tokyo 113-8602, Japan.
FAU - Shimizu, Masumi
AU  - Shimizu M
AD  - Department of Microbiology and Immunology, Nippon Medical School, 1-1-5 Sendagi, 
      Bunkyo city, Tokyo 113-8602, Japan.
FAU - Owaki, Atsuko
AU  - Owaki A
AD  - Department of Microbiology and Immunology, Nippon Medical School, 1-1-5 Sendagi, 
      Bunkyo city, Tokyo 113-8602, Japan.
FAU - Paoletti, Samantha
AU  - Paoletti S
AD  - Experimental Immunology, Department of Biomedicine, University Hospital Basel, 
      Hebelstrasse 20, CH-4031 Basel, Switzerland.
FAU - Mori, Lucia
AU  - Mori L
AD  - Experimental Immunology, Department of Biomedicine, University Hospital Basel, 
      Hebelstrasse 20, CH-4031 Basel, Switzerland.
FAU - De Libero, Gennaro
AU  - De Libero G
AD  - Experimental Immunology, Department of Biomedicine, University Hospital Basel, 
      Hebelstrasse 20, CH-4031 Basel, Switzerland.
FAU - Takahashi, Hidemi
AU  - Takahashi H
AD  - Department of Microbiology and Immunology, Nippon Medical School, 1-1-5 Sendagi, 
      Bunkyo city, Tokyo 113-8602, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151114
PL  - United States
TA  - Virology
JT  - Virology
JID - 0110674
RN  - 0 (Antigens, CD1)
RN  - 0 (CD1a antigen)
RN  - 0 (nef Gene Products, Human Immunodeficiency Virus)
RN  - 0 (nef protein, Human immunodeficiency virus 1)
RN  - EC 2.7.10.2 (HCK protein, human)
RN  - EC 2.7.10.2 (Proto-Oncogene Proteins c-hck)
RN  - EC 2.7.11.1 (PAK2 protein, human)
RN  - EC 2.7.11.1 (p21-Activated Kinases)
SB  - IM
MH  - Antigen Presentation/*immunology
MH  - Antigens, CD1/biosynthesis/*immunology
MH  - Cells, Cultured
MH  - Dendritic Cells/*immunology
MH  - Down-Regulation
MH  - HIV Infections/immunology/virology
MH  - HIV-1/immunology/pathogenicity
MH  - Humans
MH  - Proto-Oncogene Proteins c-hck/*metabolism
MH  - nef Gene Products, Human Immunodeficiency Virus/genetics/*immunology
MH  - p21-Activated Kinases/*metabolism
OTO - NOTNLM
OT  - CD1a
OT  - Dendritic cells
OT  - HIV-1 Nef
OT  - Hemopoietic cell kinase
OT  - Lipid antigens
OT  - P-21 activated kinase 2
OT  - Sulfatide
EDAT- 2015/11/20 06:00
MHDA- 2016/04/26 06:00
CRDT- 2015/11/20 06:00
PHST- 2015/07/23 00:00 [received]
PHST- 2015/10/21 00:00 [revised]
PHST- 2015/10/24 00:00 [accepted]
PHST- 2015/11/20 06:00 [entrez]
PHST- 2015/11/20 06:00 [pubmed]
PHST- 2016/04/26 06:00 [medline]
AID - S0042-6822(15)00451-1 [pii]
AID - 10.1016/j.virol.2015.10.023 [doi]
PST - ppublish
SO  - Virology. 2016 Jan;487:285-95. doi: 10.1016/j.virol.2015.10.023. Epub 2015 Nov 
      14.