PMID- 26588084
OWN - NLM
STAT- MEDLINE
DCOM- 20170313
LR  - 20220318
IS  - 1369-1635 (Electronic)
IS  - 0953-7104 (Linking)
VI  - 27
IP  - 4
DP  - 2016 Jun
TI  - Platelets attenuate production of cytokines and nitric oxide by macrophages in 
      response to bacterial endotoxin.
PG  - 344-50
LID - 10.3109/09537104.2015.1103369 [doi]
AB  - Considerable evidence has been accumulated concerning the roles of platelets in 
      immune responses. In the present study, we examined the functional modulation of 
      macrophages by platelets. When mouse bone marrow-derived macrophages (BMDMs) were 
      co-cultured with platelets, BMDMs produced lower levels of nitric oxide (NO), 
      tumor necrosis factor-alpha (TNF)-alpha, and interleukin (IL)-6 in response to a 
      bacterial endotoxin (LPS) and zymosan. The attenuation in the macrophage 
      susceptibility to LPS appeared to be mediated by soluble factors secreted from 
      platelets. The mRNA levels of NOS2 (iNOS), TNF-alpha, and IL-6 in LPS-stimulated 
      BMDMs that had been cultured with a conditioned medium of platelets were also 
      decreased as analyzed by RT-qPCR. The ability of the platelet-conditioned medium 
      to suppress macrophage NO production was recovered in a high-molecular-weight 
      fraction (>670 kDa) after gel-filtration chromatography on a Superose 6 column. 
      These results suggest that platelets control the susceptibility of macrophages to 
      prevent excessive responses to LPS and provide mechanistic insight into a 
      previous report that experimental thrombocytopenia aggravated organ failure in 
      LPS-induced endotoxemia.
FAU - Ando, Yusuke
AU  - Ando Y
AD  - a Department of Microbiology , Hoshi University School of Pharmacy and 
      Pharmaceutical Sciences , Tokyo , Japan.
FAU - Oku, Teruaki
AU  - Oku T
AD  - a Department of Microbiology , Hoshi University School of Pharmacy and 
      Pharmaceutical Sciences , Tokyo , Japan.
FAU - Tsuji, Tsutomu
AU  - Tsuji T
AD  - a Department of Microbiology , Hoshi University School of Pharmacy and 
      Pharmaceutical Sciences , Tokyo , Japan.
LA  - eng
PT  - Journal Article
DEP - 20151120
PL  - England
TA  - Platelets
JT  - Platelets
JID - 9208117
RN  - 0 (Culture Media, Conditioned)
RN  - 0 (Cytokines)
RN  - 0 (Endotoxins)
RN  - 0 (Lipopolysaccharides)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - 9010-72-4 (Zymosan)
RN  - EC 3.4.21.5 (Thrombin)
SB  - IM
MH  - Animals
MH  - Blood Platelets/*metabolism
MH  - Cell Line
MH  - Culture Media, Conditioned/pharmacology
MH  - Cytokines/*biosynthesis
MH  - Endotoxins/*metabolism
MH  - Humans
MH  - Lipopolysaccharides/immunology
MH  - Macrophage Activation/immunology
MH  - Macrophages/drug effects/*immunology/*metabolism
MH  - Mice
MH  - Nitric Oxide/*biosynthesis
MH  - Thrombin/pharmacology
MH  - Zymosan/immunology
OTO - NOTNLM
OT  - Bacterial endotoxin
OT  - cytokines
OT  - macrophage
OT  - nitric oxide
OT  - platelet
EDAT- 2015/11/21 06:00
MHDA- 2017/03/14 06:00
CRDT- 2015/11/21 06:00
PHST- 2015/11/21 06:00 [entrez]
PHST- 2015/11/21 06:00 [pubmed]
PHST- 2017/03/14 06:00 [medline]
AID - 10.3109/09537104.2015.1103369 [doi]
PST - ppublish
SO  - Platelets. 2016 Jun;27(4):344-50. doi: 10.3109/09537104.2015.1103369. Epub 2015 
      Nov 20.