PMID- 26589318
OWN - NLM
STAT- MEDLINE
DCOM- 20170414
LR  - 20220310
IS  - 1741-0444 (Electronic)
IS  - 0140-0118 (Linking)
VI  - 54
IP  - 10
DP  - 2016 Oct
TI  - A quantitative study of nanoparticle skin penetration with interactive 
      segmentation.
PG  - 1469-79
LID - 10.1007/s11517-015-1405-5 [doi]
AB  - In the last decade, the application of nanotechnology techniques has expanded 
      within diverse areas such as pharmacology, medicine, and optical science. Despite 
      such wide-ranging possibilities for implementation into practice, the mechanisms 
      behind nanoparticle skin absorption remain unknown. Moreover, the main mode of 
      investigation has been qualitative analysis. Using interactive segmentation, this 
      study suggests a method of objectively and quantitatively analyzing the 
      mechanisms underlying the skin absorption of nanoparticles. Silica nanoparticles 
      (SNPs) were assessed using transmission electron microscopy and applied to the 
      human skin equivalent model. Captured fluorescence images of this model were used 
      to evaluate degrees of skin penetration. These images underwent interactive 
      segmentation and image processing in addition to statistical quantitative 
      analyses of calculated image parameters including the mean, integrated density, 
      skewness, kurtosis, and area fraction. In images from both groups, the 
      distribution area and intensity of fluorescent silica gradually increased in 
      proportion to time. Since statistical significance was achieved after 2 days in 
      the negative charge group and after 4 days in the positive charge group, there is 
      a periodic difference. Furthermore, the quantity of silica per unit area showed a 
      dramatic change after 6 days in the negative charge group. Although this 
      quantitative result is identical to results obtained by qualitative assessment, 
      it is meaningful in that it was proven by statistical analysis with quantitation 
      by using image processing. The present study suggests that the surface charge of 
      SNPs could play an important role in the percutaneous absorption of NPs. These 
      findings can help achieve a better understanding of the percutaneous transport of 
      NPs. In addition, these results provide important guidance for the design of NPs 
      for biomedical applications.
FAU - Lee, Onseok
AU  - Lee O
AD  - Department of Medical IT Engineering, College of Medical Sciences, Soonchunhyang 
      University, Asan City, Chungnam, Korea.
FAU - Lee, See Hyun
AU  - Lee SH
AD  - Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology, Korea 
      University College of Medicine, Seoul, Korea.
AD  - Division of the Brain Korea 21 Project for Biomedical Science, Korea University 
      College of Medicine, Seoul, Korea.
FAU - Jeong, Sang Hoon
AU  - Jeong SH
AD  - Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology, Korea 
      University College of Medicine, Seoul, Korea.
AD  - Division of the Brain Korea 21 Project for Biomedical Science, Korea University 
      College of Medicine, Seoul, Korea.
FAU - Kim, Jaeyoung
AU  - Kim J
AD  - Research Institute for Skin Image, Korea University College of Medicine, Seoul, 
      Korea.
FAU - Ryu, Hwa Jung
AU  - Ryu HJ
AD  - Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology, Korea 
      University College of Medicine, Seoul, Korea.
AD  - Division of the Brain Korea 21 Project for Biomedical Science, Korea University 
      College of Medicine, Seoul, Korea.
FAU - Oh, Chilhwan
AU  - Oh C
AD  - Research Institute for Skin Image, Korea University College of Medicine, Seoul, 
      Korea.
FAU - Son, Sang Wook
AU  - Son SW
AD  - Laboratory of Cell Signaling and Nanomedicine, Department of Dermatology, Korea 
      University College of Medicine, Seoul, Korea. skin4u@korea.ac.kr.
AD  - Division of the Brain Korea 21 Project for Biomedical Science, Korea University 
      College of Medicine, Seoul, Korea. skin4u@korea.ac.kr.
AD  - Research Institute for Skin Image, Korea University College of Medicine, Seoul, 
      Korea. skin4u@korea.ac.kr.
AD  - Department of Dermatology, Korea University Anam Hospital, Korea University 
      College of Medicine, 126-1, Anam-dong 5-ga, Seongbuk-gu, Seoul, 136-705, Korea. 
      skin4u@korea.ac.kr.
LA  - eng
PT  - Journal Article
DEP - 20151120
PL  - United States
TA  - Med Biol Eng Comput
JT  - Medical & biological engineering & computing
JID - 7704869
RN  - 7631-86-9 (Silicon Dioxide)
SB  - IM
MH  - Humans
MH  - Microscopy, Electron, Transmission
MH  - Microscopy, Fluorescence
MH  - *Nanoparticles
MH  - Silicon Dioxide
MH  - Skin/*metabolism
OTO - NOTNLM
OT  - Human skin equivalent model
OT  - Nanoparticles
OT  - Segmentation
OT  - Skin penetration
EDAT- 2015/11/22 06:00
MHDA- 2017/04/15 06:00
CRDT- 2015/11/22 06:00
PHST- 2015/02/21 00:00 [received]
PHST- 2015/10/03 00:00 [accepted]
PHST- 2015/11/22 06:00 [entrez]
PHST- 2015/11/22 06:00 [pubmed]
PHST- 2017/04/15 06:00 [medline]
AID - 10.1007/s11517-015-1405-5 [pii]
AID - 10.1007/s11517-015-1405-5 [doi]
PST - ppublish
SO  - Med Biol Eng Comput. 2016 Oct;54(10):1469-79. doi: 10.1007/s11517-015-1405-5. 
      Epub 2015 Nov 20.