PMID- 2658990
OWN - NLM
STAT- MEDLINE
DCOM- 19890710
LR  - 20190612
IS  - 0006-291X (Print)
IS  - 0006-291X (Linking)
VI  - 160
IP  - 3
DP  - 1989 May 15
TI  - Transforming growth factor alpha stimulates prostacyclin production by cultured 
      human vascular endothelial cells more potently than epidermal growth factor.
PG  - 1100-5
AB  - Transforming growth factor alpha (TGF alpha) induces dose- and time-dependent 
      stimulation of prostacyclin (PGI2) production by cultured human umbilical vein 
      endothelial cells. The lowest stimulatory concentration of TGF alpha was 0.1 
      ng/ml and the maximal response, a 2.7-fold rise, was obtained with 10 ng/ml. The 
      stimulation, which lasted at least 24 h, was blocked by cycloheximide and by 
      indomethacin. TGF alpha induced PGI2 production at 10-100 times lower 
      concentrations than did epidermal growth factor (EGF), although in stimulating 
      endothelial cell growth the two factors were equipotent. This is the first 
      demonstration that TGF alpha enhances PGI2 production by human cells. Moreover, 
      this is the first evidence that it acts as both an agonist (growth) and a 
      superagonist (PGI2 production) of EGF in the same cell type. I suggest that this 
      phenomenon may be involved with the angiogenic activity of TGF alpha.
FAU - Ristimaki, A
AU  - Ristimaki A
AD  - Children's Hospital, University of Helsinki, Finland.
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 58962-34-8 (6-Ketoprostaglandin F1 alpha)
RN  - 62229-50-9 (Epidermal Growth Factor)
RN  - 76057-06-2 (Transforming Growth Factors)
RN  - 98600C0908 (Cycloheximide)
RN  - DCR9Z582X0 (Epoprostenol)
RN  - XXE1CET956 (Indomethacin)
SB  - IM
MH  - 6-Ketoprostaglandin F1 alpha/biosynthesis
MH  - Cycloheximide/pharmacology
MH  - Dose-Response Relationship, Drug
MH  - Endothelium, Vascular/drug effects/*metabolism
MH  - Epidermal Growth Factor/pharmacology
MH  - Epoprostenol/*biosynthesis
MH  - Humans
MH  - Indomethacin/pharmacology
MH  - Kinetics
MH  - Transforming Growth Factors/*pharmacology
MH  - Umbilical Veins
EDAT- 1989/05/15 00:00
MHDA- 1989/05/15 00:01
CRDT- 1989/05/15 00:00
PHST- 1989/05/15 00:00 [pubmed]
PHST- 1989/05/15 00:01 [medline]
PHST- 1989/05/15 00:00 [entrez]
AID - S0006-291X(89)80116-0 [pii]
AID - 10.1016/s0006-291x(89)80116-0 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 1989 May 15;160(3):1100-5. doi: 
      10.1016/s0006-291x(89)80116-0.