PMID- 26590300
OWN - NLM
STAT- MEDLINE
DCOM- 20161027
LR  - 20200601
IS  - 1756-2651 (Electronic)
IS  - 0021-924X (Print)
IS  - 0021-924X (Linking)
VI  - 159
IP  - 4
DP  - 2016 Apr
TI  - 5-Aminoimidazole-4-carboxyamide-1-beta-D-ribofranoside stimulates the rat enhancer 
      of split- and hairy-related protein-2 gene via atypical protein kinase C lambda.
PG  - 429-36
LID - 10.1093/jb/mvv116 [doi]
AB  - The 5'-AMP-activated protein kinase (AMPK) functions as a cellular energy sensor. 
      5-Aminoimidazole-4-carboxyamide-1-beta-D-ribofranoside (AICAR) is a chemical 
      activator of AMPK. In the liver, AICAR suppresses expression of 
      thephosphoenolpyruvate carboxykinase(PEPCK) gene. The rat enhancer of split- and 
      hairy-related protein-2 (SHARP-2) is an insulin-inducible transcriptional 
      repressor and its target is thePEPCKgene. In this study, we examined an issue of 
      whether theSHARP-2gene expression is regulated by AICAR via the AMPK. AICAR 
      increased the level of SHARP-2 mRNA in H4IIE cells. Whereas an AMPK inhibitor, 
      compound-C, had no effects on the AICAR-induction, inhibitors for both 
      phosphoinositide 3-kinase (PI 3-K) and protein kinase C (PKC) completely 
      diminished the effects of AICAR. Western blot analyses showed that AICAR rapidly 
      activated atypical PKC lambda (aPKClambda). In addition, when a dominant negative form 
      of aPKClambda was expressed, the induction of SHARP-2 mRNA level by AICAR was 
      inhibited. Calcium ion is not required for the activation of aPKClambda. A calcium 
      ion-chelating reagent had no effects on the AICAR-induction. Furthermore, the 
      AICAR-induction was inhibited by treatment with an RNA polymerase inhibitor or a 
      protein synthesis inhibitor. Thus, we conclude that the AICAR-induction of 
      theSHARP-2gene is mediated at transcription level by a PI 3-K/aPKClambda pathway.
CI  - (c) The Authors 2015. Published by Oxford University Press on behalf of the 
      Japanese Biochemical Society. All rights reserved.
FAU - Komatsu, Yoshiko
AU  - Komatsu Y
AD  - Matsumoto University Graduate School of Health Science, 2095-1 Niimura, 
      Matsumoto, Nagano 390-1295, Japan;
FAU - Yanagisawa, Yuki
AU  - Yanagisawa Y
AD  - Matsumoto University Graduate School of Health Science, 2095-1 Niimura, 
      Matsumoto, Nagano 390-1295, Japan;
FAU - Moriizumi, Maya
AU  - Moriizumi M
AD  - Department of Health and Nutritional Science, Faculty of Human Health Science, 
      Matsumoto University, 2095-1 Niimura, Matsumoto, Nagano 390-1295, Japan;
FAU - Tsuchiya, Yuuki
AU  - Tsuchiya Y
AD  - Department of Health and Nutritional Science, Faculty of Human Health Science, 
      Matsumoto University, 2095-1 Niimura, Matsumoto, Nagano 390-1295, Japan;
FAU - Yokouchi, Honami
AU  - Yokouchi H
AD  - Department of Health and Nutritional Science, Faculty of Human Health Science, 
      Matsumoto University, 2095-1 Niimura, Matsumoto, Nagano 390-1295, Japan;
FAU - Otsuka, Hatsumi
AU  - Otsuka H
AD  - Department of Health and Nutritional Science, Faculty of Human Health Science, 
      Matsumoto University, 2095-1 Niimura, Matsumoto, Nagano 390-1295, Japan;
FAU - Aoyagi, Mizuki
AU  - Aoyagi M
AD  - Department of Health and Nutritional Science, Faculty of Human Health Science, 
      Matsumoto University, 2095-1 Niimura, Matsumoto, Nagano 390-1295, Japan;
FAU - Tsukada, Akiko
AU  - Tsukada A
AD  - Matsumoto University Graduate School of Health Science, 2095-1 Niimura, 
      Matsumoto, Nagano 390-1295, Japan;
FAU - Kanai, Yukiko
AU  - Kanai Y
AD  - Matsumoto University Graduate School of Health Science, 2095-1 Niimura, 
      Matsumoto, Nagano 390-1295, Japan;
FAU - Haneishi, Ayumi
AU  - Haneishi A
AD  - Department of Health and Nutritional Science, Faculty of Human Health Science, 
      Matsumoto University, 2095-1 Niimura, Matsumoto, Nagano 390-1295, Japan;
FAU - Takagi, Katsuhiro
AU  - Takagi K
AD  - Matsumoto University Graduate School of Health Science, 2095-1 Niimura, 
      Matsumoto, Nagano 390-1295, Japan; Department of Health and Nutritional Science, 
      Faculty of Human Health Science, Matsumoto University, 2095-1 Niimura, Matsumoto, 
      Nagano 390-1295, Japan;
FAU - Asano, Kosuke
AU  - Asano K
AD  - Department of Health and Nutritional Science, Faculty of Human Health Science, 
      Matsumoto University, 2095-1 Niimura, Matsumoto, Nagano 390-1295, Japan;
FAU - Ono, Moe
AU  - Ono M
AD  - Matsumoto University Graduate School of Health Science, 2095-1 Niimura, 
      Matsumoto, Nagano 390-1295, Japan; Laboratory of Molecular Biology, Faculty of 
      Pharmacy, Osaka Ohtani University, 3-11-1 Nishikiorikita, Tondabayashi, Osaka 
      584-8540, Japan.
FAU - Tanaka, Takashi
AU  - Tanaka T
AD  - Laboratory of Molecular Biology, Faculty of Pharmacy, Osaka Ohtani University, 
      3-11-1 Nishikiorikita, Tondabayashi, Osaka 584-8540, Japan.
FAU - Tomita, Koji
AU  - Tomita K
AD  - Laboratory of Molecular Biology, Faculty of Pharmacy, Osaka Ohtani University, 
      3-11-1 Nishikiorikita, Tondabayashi, Osaka 584-8540, Japan.
FAU - Yamada, Kazuya
AU  - Yamada K
AD  - Matsumoto University Graduate School of Health Science, 2095-1 Niimura, 
      Matsumoto, Nagano 390-1295, Japan; Department of Health and Nutritional Science, 
      Faculty of Human Health Science, Matsumoto University, 2095-1 Niimura, Matsumoto, 
      Nagano 390-1295, Japan; kazuya.yamada@matsu.ac.jp.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151120
PL  - England
TA  - J Biochem
JT  - Journal of biochemistry
JID - 0376600
RN  - 0 (Basic Helix-Loop-Helix Transcription Factors)
RN  - 0 (Bhlhe40 protein, rat)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (Intracellular Signaling Peptides and Proteins)
RN  - 0 (Isoenzymes)
RN  - 0 (Phosphoinositide-3 Kinase Inhibitors)
RN  - 0 (Protein Synthesis Inhibitors)
RN  - 0 (Pyrazoles)
RN  - 0 (Pyrimidines)
RN  - 0 (RNA, Messenger)
RN  - 0 (Ribonucleosides)
RN  - 10K52CIC1Z (dorsomorphin)
RN  - 1CC1JFE158 (Dactinomycin)
RN  - 360-97-4 (Aminoimidazole Carboxamide)
RN  - 53IEF47846 (acadesine)
RN  - 98600C0908 (Cycloheximide)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - EC 2.7.11.13 (protein kinase C lambda)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
RN  - EC 2.7.7.- (RNA Polymerase II)
RN  - EC 4.1.1.32 (Pck1 protein, rat)
RN  - EC 4.1.1.32 (Phosphoenolpyruvate Carboxykinase (GTP))
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - AMP-Activated Protein Kinases/antagonists & inhibitors/genetics/*metabolism
MH  - Aminoimidazole Carboxamide/*analogs & derivatives/pharmacology
MH  - Animals
MH  - Basic Helix-Loop-Helix Transcription Factors/*genetics
MH  - Calcium/metabolism
MH  - Cycloheximide/pharmacology
MH  - Dactinomycin/pharmacology
MH  - Enzyme Activation
MH  - Gene Expression/*drug effects
MH  - HEK293 Cells
MH  - Homeodomain Proteins/*genetics
MH  - Humans
MH  - Intracellular Signaling Peptides and Proteins/metabolism
MH  - Isoenzymes/antagonists & inhibitors/genetics/*metabolism
MH  - Liver/enzymology
MH  - Phosphatidylinositol 3-Kinases/metabolism
MH  - Phosphoenolpyruvate Carboxykinase (GTP)/metabolism
MH  - Phosphoinositide-3 Kinase Inhibitors
MH  - Protein Kinase C/antagonists & inhibitors/genetics/*metabolism
MH  - Protein Synthesis Inhibitors/pharmacology
MH  - Pyrazoles/pharmacology
MH  - Pyrimidines/pharmacology
MH  - RNA Polymerase II/antagonists & inhibitors
MH  - RNA, Messenger/metabolism
MH  - Rats
MH  - Ribonucleosides/*pharmacology
MH  - Signal Transduction
MH  - Transcription, Genetic/drug effects
PMC - PMC4885932
OTO - NOTNLM
OT  - 5'-AMP-activated protein kinase
OT  - AICAR
OT  - SHARP-2
OT  - atypical protein kinase C lambda
OT  - insulin-inducible transcriptional repressor
EDAT- 2015/11/22 06:00
MHDA- 2016/11/01 06:00
PMCR- 2017/04/01
CRDT- 2015/11/22 06:00
PHST- 2015/09/10 00:00 [received]
PHST- 2015/10/21 00:00 [accepted]
PHST- 2015/11/22 06:00 [entrez]
PHST- 2015/11/22 06:00 [pubmed]
PHST- 2016/11/01 06:00 [medline]
PHST- 2017/04/01 00:00 [pmc-release]
AID - mvv116 [pii]
AID - 10.1093/jb/mvv116 [doi]
PST - ppublish
SO  - J Biochem. 2016 Apr;159(4):429-36. doi: 10.1093/jb/mvv116. Epub 2015 Nov 20.