PMID- 26591366
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20181202
IS  - 1003-5370 (Print)
IS  - 1003-5370 (Linking)
VI  - 35
IP  - 9
DP  - 2015 Sep
TI  - [Yinxingye Capsule Intervened Vascular Endothelial Cell Apoptosis of 
      Hyperhomocysteinemia Rats: an Experimental Study].
PG  - 1099-104
AB  - OBJECTIVE: To explore targets of Chinese herbal medicine at cellular and 
      molecular leve1s through an experimental study on Yinxingye Capsule (YC) 
      intervening vascular endothelial cell apoptoeis of hyperhornocysteinemia (HHcy) 
      rats. METHODS: The HHcy model was prepared in male Wistar rats. Totally 42 rats 
      were randomly divided into 4 groups, i.e., the control group (n =10), the model 
      group (n = 11), the YC group (n =11), the folic acid group (n =10). Carboxy 
      methyl cellulose (CMC) solution (1%) was administered to rats in the control 
      group by gastrogavage.3% methionine suspension at 1. 5 g/kg was administered to 
      rats in the model group by gastrogavage. 3% methionine suspension at 1. 5 g/kg 
      and folic acid suspension at 0. 06 g/kg was administered to rats in the folic 
      acid group by gastrogavage. 3% methionine suspension at 1. 5 g/kg and YC at 0. 02 
      g/kg was administered to rats in the YC group by gastrogavage. Morphological 
      changes of aortic tissue were observed by hematoxylin eosin (HE) staining. The 
      plasma homocysteine (Hcy) level was detected in each group. The 
      endothelium-dependent diastolic functions of the thoracic aorta on different 
      concentrations of sodium nitroprusside (SNP) and acetylcholine (Ach) were 
      detected. Gene expressions of Bcl-2-associated X protein (BAX), inducible nitric 
      oxide synthase (iNOS), c-Fos, cellular inhibitor of apoptosis protein 2 (c-IAP2) 
      were detected by real time polymerase chain reaction (RT-PCR). RESULTS: 
      Pathological results showed that thickening aortic endothelium, swollen and 
      desquamated endothelial cells. Few foam cells could be seen in the model group. 
      Myoma-like proliferation of smooth muscle cells in tunica media could also be 
      seen. These pathological changes were milder in the YC group and the folic acid 
      group. Compared with the control group, plasma Hcy levels increased in the model 
      group (P <0. 05). The endothelium-dependent diastolic rates at 10(-6) and 
      10(-4)mol/L Ach and 10(-7) -10(-3)mol/L SNP all decreased in the model group (P 
      <0. 01, P <0. 05). Gene expressions of Bax, c-Fos, and iNOS increased, but c-IAP2 
      gene expressions decreased in the model group (all P <0. 05). Compared with the 
      model group, plasma Hcy levels decreased in the YC group and the folic acid group 
      (P <0. 05). The endothelium-dependent diastolic rates increased in the YC group 
      and the folic acid group at various SNP concentrations except 10(-6) mol/L SNP in 
      the folic acid group. The endothelium-dependent diastolic rates increased in the 
      YC group and the folic acid group at 10(-6) and 10(-4)mol/L Ach (all P <0. 05). 
      Gene expressions of Bax, c-Fos, and iNOS decreased in the YC group and the folic 
      acid group, but c-IAP2 gene expression increased in the folic acid group (all P 
      <0. 05). CONCLUSION: YC could reduce plasma Hcy levels, down-regulate gene 
      expressions of Bax, c-Fos, and iNOS, thereby reducing apoptosis of vascular 
      endothelial cells, improving vascular endothelial function, and delaying 
      atherosclerotic process.
FAU - Xu, Zhi-bing
AU  - Xu ZB
FAU - Wang, Wei-dong
AU  - Wang WD
FAU - Zhang, Li-fen
AU  - Zhang LF
FAU - Li, Jun
AU  - Li J
FAU - Wang, Yi
AU  - Wang Y
FAU - Xi, Xi-xiang
AU  - Xi XX
FAU - Zhu, Jie
AU  - Zhu J
FAU - Ma, Jin-miao
AU  - Ma JM
FAU - Jia, Jing-ying
AU  - Jia JY
FAU - Zhang, Li-wei
AU  - Zhang LW
FAU - Gu, Ren-yue
AU  - Gu RY
LA  - chi
PT  - Journal Article
PL  - China
TA  - Zhongguo Zhong Xi Yi Jie He Za Zhi
JT  - Zhongguo Zhong xi yi jie he za zhi Zhongguo Zhongxiyi jiehe zazhi = Chinese 
      journal of integrated traditional and Western medicine
JID - 9211576
RN  - 0 (Drugs, Chinese Herbal)
RN  - 0 (Proto-Oncogene Proteins c-fos)
RN  - 0 (bcl-2-Associated X Protein)
RN  - 169D1260KM (Nitroprusside)
RN  - EC 1.14.13.39 (NOS2 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type II)
RN  - N9YNS0M02X (Acetylcholine)
SB  - IM
MH  - Acetylcholine
MH  - Animals
MH  - Aorta
MH  - Aorta, Thoracic
MH  - Apoptosis/drug effects
MH  - Drugs, Chinese Herbal/*pharmacology/therapeutic use
MH  - Endothelial Cells
MH  - Endothelium, Vascular
MH  - Hyperhomocysteinemia/*drug therapy
MH  - Male
MH  - Nitric Oxide Synthase Type II
MH  - Nitroprusside
MH  - Proto-Oncogene Proteins c-fos
MH  - Rats
MH  - Rats, Wistar
MH  - bcl-2-Associated X Protein
EDAT- 2015/11/26 06:00
MHDA- 2016/09/16 06:00
CRDT- 2015/11/24 06:00
PHST- 2015/11/24 06:00 [entrez]
PHST- 2015/11/26 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
PST - ppublish
SO  - Zhongguo Zhong Xi Yi Jie He Za Zhi. 2015 Sep;35(9):1099-104.