PMID- 2659162
OWN - NLM
STAT- MEDLINE
DCOM- 19890727
LR  - 20071114
IS  - 0008-5472 (Print)
IS  - 0008-5472 (Linking)
VI  - 49
IP  - 13
DP  - 1989 Jul 1
TI  - Effect in vivo of multiple injections of purified murine and recombinant human 
      macrophage colony-stimulating factor to mice.
PG  - 3558-61
AB  - Hematopoietic efficacy in vivo of multiple injections of purified murine L-cell 
      and recombinant human macrophage colony-stimulating factors (M-CSF; specific 
      activity, greater than 2 x 10(7) units/mg) was assessed in mice. Injections i.v. 
      of sterile saline or 20,000 units of M-CSF were administered once (at 0 h), twice 
      (at 0 and 12 h), or three times (at 0, 12, and 24 h) to C57BL/6 x DBA/2 F1 mice. 
      Numbers and cycling rates of marrow and spleen granulocyte-macrophage, erythroid, 
      and multipotential progenitor cells were assessed 32-36 h after the first 
      injection. Marrow, spleen, and peripheral blood cellularity was assessed at 
      intervals of up to 105 h. Progenitor cell cycling rates were significantly 
      increased after one and two injections of M-CSF but were reduced to a slow or 
      noncycling state after three injections. For marrow cells, the third injection 
      resulted in a significant suppression of hematopoietic progenitor cell cycling 
      compared to the control group. No significant changes were noted for number of 
      progenitors per femur or spleen, for marrow, spleen, or peripheral blood 
      cellularity, or for differential cell counts in these organs after any of the 
      M-CSF treatment schedules. Suppression of progenitor cell proliferation noted 
      after three injections of M-CSF may at least partially explain why repeated 
      injections of 20,000 units of M-CSF fails to increase bone marrow, spleen, or 
      blood cellularity even though one injection of M-CSF increases cycling rates of 
      the hematopoietic progenitors.
FAU - Chikkappa, G
AU  - Chikkappa G
AD  - Medical Service, VA Medical Center, Albany, New York 12208.
FAU - Broxmeyer, H E
AU  - Broxmeyer HE
FAU - Cooper, S
AU  - Cooper S
FAU - Williams, D E
AU  - Williams DE
FAU - Hangoc, G
AU  - Hangoc G
FAU - Greenberg, M L
AU  - Greenberg ML
FAU - Waheed, A
AU  - Waheed A
FAU - Shadduck, R K
AU  - Shadduck RK
LA  - eng
GR  - CA 15237/CA/NCI NIH HHS/United States
GR  - CA 36464/CA/NCI NIH HHS/United States
GR  - CA 36740/CA/NCI NIH HHS/United States
GR  - etc.
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PT  - Research Support, U.S. Gov't, Non-P.H.S.
PT  - Research Support, U.S. Gov't, P.H.S.
PL  - United States
TA  - Cancer Res
JT  - Cancer research
JID - 2984705R
RN  - 0 (Colony-Stimulating Factors)
RN  - 0 (Recombinant Proteins)
RN  - 81627-83-0 (Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells
MH  - Cell Cycle/drug effects
MH  - Colony-Stimulating Factors/administration & dosage/*pharmacology
MH  - Hematopoiesis/drug effects
MH  - Leukocyte Count/drug effects
MH  - Macrophage Colony-Stimulating Factor
MH  - Mice
MH  - Recombinant Proteins
MH  - Spleen/cytology
MH  - Time Factors
EDAT- 1989/07/01 00:00
MHDA- 1989/07/01 00:01
CRDT- 1989/07/01 00:00
PHST- 1989/07/01 00:00 [pubmed]
PHST- 1989/07/01 00:01 [medline]
PHST- 1989/07/01 00:00 [entrez]
PST - ppublish
SO  - Cancer Res. 1989 Jul 1;49(13):3558-61.