PMID- 26592657
OWN - NLM
STAT- MEDLINE
DCOM- 20160912
LR  - 20181202
IS  - 1873-3573 (Electronic)
IS  - 0039-9140 (Linking)
VI  - 147
DP  - 2016 Jan 15
TI  - Development of magnetic molecularly imprinted polymers for solid phase extraction 
      of cocaine and metabolites in urine before high performance liquid chromatography 
      - tandem mass spectrometry.
PG  - 641-9
LID - S0039-9140(15)30399-4 [pii]
LID - 10.1016/j.talanta.2015.10.034 [doi]
AB  - A magnetic molecularly imprinted polymer (MMIP) has been synthesized and applied 
      for cocaine (COC) and metabolites (benzoylecgonine, BZE; cocaethylene, CE; and 
      ecgonine methyl ester, EME) recognition/pre-concentration in urine samples. The 
      MMIP has been prepared using COC as a template molecule, ethylene dimethacrylate 
      (EDMA) as a functional monomer, divinylbenzene (DVB) as a cross-linker, Fe3O4 
      magnetite as a magnetic component, and 2,2'-azobisisobutyronitrile (AIBN) as an 
      initiator. The best results (MIP layer on the surface of the magnetic 
      nanoparticles) and physical properties of the prepared MMIP were obtained when 
      assisting the synthesis procedure with ultrasounds (325W, 37kHz, 30 degrees C, 4h). After 
      solid phase extraction (SPE) with the prepared adsorbent material, analytes were 
      determined by high performance liquid chromatography - tandem mass spectrometry 
      (HPLC-MS/MS). Variables affecting the SPE process (batch mode) were fully 
      evaluated. Optimum retention of analytes (1.8mL of urine and 50mg of MMIP) was 
      achieved by fixing the urine pH at 5.5 (use of a KH2PO4/NaOH, pH 5.5 buffer 
      solution), and magnetic stirring (25 degrees C, 700rpm) for 10min. Elution was performed 
      by using 2mL of a dichloromethane/2-propanol/ammonium hydroxide (75:20:5) mixture 
      under ultrasounds (325W, 35kHz, room temperature) for 5min. The method was 
      validated according to the guidance for bioanalytical method validation of the US 
      Department of Health and Human Services, Food and Drug Administration. The 
      detection limits were in the range of 0.39-1.4ngL(-1). The relative standard 
      deviations of intra- and inter-day tests ranged from 5 to 11% and from 3 to 11%, 
      respectively. Analytical recoveries were in the range of 79-106% when spiking 
      drug-free urine samples at three concentration levels. Good results were also 
      obtained after analyzing an FDT +25% control material. The applicability of the 
      method was proved for screening/quantifying COC, BZE, CE and EME in several 
      samples from poly-drug abusers.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Sanchez-Gonzalez, Juan
AU  - Sanchez-Gonzalez J
AD  - Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n, 15782 
      Santiago de Compostela, Spain.
FAU - Jesus Tabernero, Maria
AU  - Jesus Tabernero M
AD  - Department of Pathologic Anatomy and Forensic Sciences, Faculty of Medicine, 
      University of Santiago de Compostela, Rua de San Francisco, s/n, 15782 Santiago 
      de Compostela, Spain.
FAU - Bermejo, Ana Maria
AU  - Bermejo AM
AD  - Department of Pathologic Anatomy and Forensic Sciences, Faculty of Medicine, 
      University of Santiago de Compostela, Rua de San Francisco, s/n, 15782 Santiago 
      de Compostela, Spain.
FAU - Bermejo-Barrera, Pilar
AU  - Bermejo-Barrera P
AD  - Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n, 15782 
      Santiago de Compostela, Spain.
FAU - Moreda-Pineiro, Antonio
AU  - Moreda-Pineiro A
AD  - Department of Analytical Chemistry, Nutrition and Bromatology, Faculty of 
      Chemistry, University of Santiago de Compostela, Avenida das Ciencias, s/n, 15782 
      Santiago de Compostela, Spain. Electronic address: antonio.moreda@usc.es.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151019
PL  - Netherlands
TA  - Talanta
JT  - Talanta
JID - 2984816R
RN  - 0 (Methacrylates)
RN  - 0 (Nitriles)
RN  - 0 (Vinyl Compounds)
RN  - 3WJQ0SDW1A (Polyethylene Glycols)
RN  - 5353I8I6YS (benzoylecgonine)
RN  - 7BK5G69305 (ethylene dimethacrylate)
RN  - FJO3071W5Y (cocaethylene)
RN  - FZ6PX8U5YB (azobis(isobutyronitrile))
RN  - I5Y540LHVR (Cocaine)
RN  - IZ715T4SBU (divinyl benzene)
RN  - XM0M87F357 (Ferrosoferric Oxide)
RN  - Y35FJB3QBJ (ecgonine methyl ester)
SB  - IM
MH  - Chromatography, High Pressure Liquid
MH  - Cocaine/*analogs & derivatives/urine
MH  - Ferrosoferric Oxide/chemistry
MH  - Humans
MH  - Limit of Detection
MH  - Magnetic Phenomena
MH  - Methacrylates/chemistry
MH  - Molecular Imprinting
MH  - Nitriles/chemistry
MH  - Polyethylene Glycols/chemistry
MH  - Solid Phase Extraction/methods
MH  - Tandem Mass Spectrometry
MH  - Ultrasonic Waves
MH  - Vinyl Compounds/chemistry
OTO - NOTNLM
OT  - Cocaine
OT  - High performance liquid chromatography tandem mass spectrometry
OT  - Human urine
OT  - Magnetic molecularly imprinted polymer
EDAT- 2015/11/26 06:00
MHDA- 2016/09/13 06:00
CRDT- 2015/11/24 06:00
PHST- 2015/07/06 00:00 [received]
PHST- 2015/10/09 00:00 [revised]
PHST- 2015/10/13 00:00 [accepted]
PHST- 2015/11/24 06:00 [entrez]
PHST- 2015/11/26 06:00 [pubmed]
PHST- 2016/09/13 06:00 [medline]
AID - S0039-9140(15)30399-4 [pii]
AID - 10.1016/j.talanta.2015.10.034 [doi]
PST - ppublish
SO  - Talanta. 2016 Jan 15;147:641-9. doi: 10.1016/j.talanta.2015.10.034. Epub 2015 Oct 
      19.