PMID- 26596846
OWN - NLM
STAT- MEDLINE
DCOM- 20160915
LR  - 20151130
IS  - 1872-9754 (Electronic)
IS  - 0197-0186 (Linking)
VI  - 91
DP  - 2015 Dec
TI  - Self-amplified BDNF transcription is a regulatory system for synaptic maturation 
      in cultured cortical neurons.
PG  - 55-61
LID - S0197-0186(15)30059-0 [pii]
LID - 10.1016/j.neuint.2015.10.009 [doi]
AB  - Neuronal cell survival and synaptic plasticity are controlled through expression 
      of various neurotrophic factors including brain-derived neurotrophic factor 
      (BDNF). In the present study, we examined the mechanism behind BDNF-induced Bdnf 
      mRNA production and the physiological role of its amplification system using 
      cortical neurons. Exogenous BDNF was applied to the cultured cortical neurons at 
      days in vitro (DIV) 3 and DIV 7 with or without inhibitors for intracellular 
      signaling. Expression levels of total Bdnf and Bdnf variants (exon I, exon IV, 
      and exon VI) were biphasically increased after the BDNF application in different 
      developing stage of neurons. Inhibitor for extracellular signal-regulated kinase, 
      calmodulin dependent protein kinase II, or protein kinase A repressed the 
      BDNF-induced Bdnf mRNA expression. Furthermore, we found that application of 
      TrkB-Fc, which scavenges produced endogenous BDNF, resulted in weakened BDNF/TrkB 
      signaling and decreased expression of postsynaptic proteins, suggesting that 
      newly synthesized BDNF induced by the self-amplification system contributes to 
      the synaptic maturation and function.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Nakajima, Shingo
AU  - Nakajima S
AD  - Department of Mental Disorder Research, National Institute of Neuroscience, 
      National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan; Faculty of 
      Health Science, Hokkaido University, Sapporo, Japan.
FAU - Numakawa, Tadahiro
AU  - Numakawa T
AD  - Department of Mental Disorder Research, National Institute of Neuroscience, 
      National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan; Department of 
      Cell Modulation, Institute of Molecular Embryology and Genetics, Kumamoto 
      University, Kumamoto, Japan. Electronic address: numakawa.yyrmk@gmail.com.
FAU - Adachi, Naoki
AU  - Adachi N
AD  - Department of Mental Disorder Research, National Institute of Neuroscience, 
      National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan; Department of 
      Biomedical Chemistry, Kwansei Gakuin University, Sanda, Japan.
FAU - Ooshima, Yoshiko
AU  - Ooshima Y
AD  - Department of Mental Disorder Research, National Institute of Neuroscience, 
      National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan; Department of 
      Molecular Pharmacology, National Institute of Neuroscience, National Center of 
      Neurology and Psychiatry (NCNP), Tokyo, Japan.
FAU - Odaka, Haruki
AU  - Odaka H
AD  - Department of Mental Disorder Research, National Institute of Neuroscience, 
      National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan; Department of 
      Life Science and Medical Bioscience, School of Advanced Science and Engineering, 
      Waseda University, Tokyo, Japan.
FAU - Yoshimura, Aya
AU  - Yoshimura A
AD  - Department of Mental Disorder Research, National Institute of Neuroscience, 
      National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan.
FAU - Kunugi, Hiroshi
AU  - Kunugi H
AD  - Department of Mental Disorder Research, National Institute of Neuroscience, 
      National Center of Neurology and Psychiatry (NCNP), Tokyo, Japan.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151024
PL  - England
TA  - Neurochem Int
JT  - Neurochemistry international
JID - 8006959
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Cyclic AMP Response Element-Binding Protein)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (RNA, Messenger)
RN  - EC 2.7.10.1 (Receptor, trkB)
SB  - IM
MH  - Animals
MH  - Brain-Derived Neurotrophic Factor/antagonists & 
      inhibitors/*biosynthesis/*genetics
MH  - Cells, Cultured
MH  - Cerebral Cortex/cytology/*metabolism
MH  - Cyclic AMP Response Element-Binding Protein/biosynthesis/genetics
MH  - Neurons/*metabolism
MH  - Protein Kinase Inhibitors/pharmacology
MH  - RNA, Messenger/biosynthesis/genetics
MH  - Rats
MH  - Rats, Wistar
MH  - Receptor, trkB/pharmacology
MH  - Signal Transduction/drug effects
MH  - Synapses/drug effects/metabolism/*physiology
OTO - NOTNLM
OT  - BDNF
OT  - CREB
OT  - ERK
OT  - TrkB
EDAT- 2015/11/26 06:00
MHDA- 2016/09/16 06:00
CRDT- 2015/11/25 06:00
PHST- 2015/02/04 00:00 [received]
PHST- 2015/10/08 00:00 [revised]
PHST- 2015/10/20 00:00 [accepted]
PHST- 2015/11/25 06:00 [entrez]
PHST- 2015/11/26 06:00 [pubmed]
PHST- 2016/09/16 06:00 [medline]
AID - S0197-0186(15)30059-0 [pii]
AID - 10.1016/j.neuint.2015.10.009 [doi]
PST - ppublish
SO  - Neurochem Int. 2015 Dec;91:55-61. doi: 10.1016/j.neuint.2015.10.009. Epub 2015 
      Oct 24.