PMID- 26597533
OWN - NLM
STAT- MEDLINE
DCOM- 20161111
LR  - 20170103
IS  - 1738-8872 (Electronic)
IS  - 1017-7825 (Linking)
VI  - 26
IP  - 2
DP  - 2016 Feb
TI  - Cytoprotective Effect of Makgeolli Lees on Paraquat Induced Oxidative Stress in 
      A549 Cells via Activation of NRF2 and Antioxidant Genes.
PG  - 277-86
LID - 10.4014/jmb.1510.10093 [doi]
AB  - Makgeolli lees (ML) has several physiological effects such as antioxidant, 
      antidiabetic, and anticancer properties, but its biological functions have not 
      been determined definitively. Here, we tested whether ML has a cytoprotective 
      effect on paraquat (PQ)-induced oxidative stress in the human lung carcinoma cell 
      line A549. At 0.1 mg/ml ML, viability of PQ-exposed A549 cells was restored by 
      12.4%, 18.5%, and 48.6% after 24, 48, and 72 h, respectively. ML also reduced 
      production of the intracellular reactive oxygen species (ROS) that were generated 
      by PQ treatment. Further experiments revealed that ML treatment enhanced the 
      expression and nuclear translocation of nuclear factor erythroid 2-related factor 
      2 (NRF2) as well as ARE-GFP reporter activity. ML treatment also effectively 
      increased the expression of NRF2's target genes NAD(P)H dehydrogenase quinone 1 
      (NQO1) and heme oxygenase 1 (HO-1). Moreover, we found that expression of 
      cytoprotective genes, including glutathione peroxidases (GPXs), superoxide 
      dismutase (SOD1), catalase (CAT), peroxiredoxin 3 (PRDX3), and peroxiredoxin 4 
      (PRDX4), was greatly enhanced by treatment with ML during PQ exposure. Taken 
      together, the data suggest that treatment of PQ-exposed A549 cells with ML 
      ameliorates cytotoxicity through induction of NRF2 expression and its target 
      genes HO-1, NQO1, and other antioxidant genes. Thus, ML may serve as a functional 
      food applicable to ROS-mediated human diseases.
FAU - Jeon, Miso
AU  - Jeon M
AD  - Department of Microbiology, College of Medicine, Soonchunhyang University, 
      Cheonan 31151, Republic of Korea.
FAU - Rahman, Naimur
AU  - Rahman N
AD  - Department of Microbiology, College of Medicine, Soonchunhyang University, 
      Cheonan 31151, Republic of Korea.
AD  - Institute of Tissue Engineering, College of Medicine, Soonchunhyang University, 
      Cheonan 31151, Republic of Korea.
FAU - Kim, Yong-Sik
AU  - Kim YS
AD  - Department of Microbiology, College of Medicine, Soonchunhyang University, 
      Cheonan 31151, Republic of Korea.
AD  - Institute of Tissue Engineering, College of Medicine, Soonchunhyang University, 
      Cheonan 31151, Republic of Korea.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - Korea (South)
TA  - J Microbiol Biotechnol
JT  - Journal of microbiology and biotechnology
JID - 9431852
RN  - 0 (Antioxidants)
RN  - 0 (Herbicides)
RN  - 0 (NF-E2-Related Factor 2)
RN  - 0 (NFE2L2 protein, human)
RN  - 0 (Reactive Oxygen Species)
RN  - EC 1.11.1.15 (PRDX3 protein, human)
RN  - EC 1.11.1.15 (PRDX4 protein, human)
RN  - EC 1.11.1.15 (Peroxiredoxin III)
RN  - EC 1.11.1.15 (Peroxiredoxins)
RN  - EC 1.11.1.6 (Catalase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - EC 1.14.14.18 (HMOX1 protein, human)
RN  - EC 1.14.14.18 (Heme Oxygenase-1)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
RN  - EC 1.6.5.2 (NAD(P)H Dehydrogenase (Quinone))
RN  - EC 1.6.5.2 (NQO1 protein, human)
RN  - PLG39H7695 (Paraquat)
SB  - IM
MH  - *Antioxidants
MH  - Catalase/genetics
MH  - Cell Line, Tumor
MH  - Cell Survival/drug effects
MH  - *Cytoprotection
MH  - Functional Food
MH  - Genes, Reporter
MH  - Glutathione Peroxidase/genetics
MH  - Heme Oxygenase-1/genetics
MH  - Herbicides/*pharmacology
MH  - Humans
MH  - NAD(P)H Dehydrogenase (Quinone)/genetics
MH  - NF-E2-Related Factor 2/*genetics/metabolism
MH  - Oxidative Stress/*drug effects
MH  - Paraquat/*pharmacology
MH  - Peroxiredoxin III/genetics
MH  - Peroxiredoxins/genetics
MH  - Reactive Oxygen Species/metabolism
MH  - Superoxide Dismutase/genetics
MH  - *Wine
OTO - NOTNLM
OT  - A549
OT  - Antioxidant response element
OT  - Makgeolli lees
OT  - NRF2
OT  - Oxidative stress
OT  - Paraquat
EDAT- 2015/11/26 06:00
MHDA- 2016/11/12 06:00
CRDT- 2015/11/25 06:00
PHST- 2015/11/25 06:00 [entrez]
PHST- 2015/11/26 06:00 [pubmed]
PHST- 2016/11/12 06:00 [medline]
AID - 10.4014/jmb.1510.10093 [pii]
AID - 10.4014/jmb.1510.10093 [doi]
PST - ppublish
SO  - J Microbiol Biotechnol. 2016 Feb;26(2):277-86. doi: 10.4014/jmb.1510.10093.