PMID- 26600478
OWN - NLM
STAT- MEDLINE
DCOM- 20160929
LR  - 20151125
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 4
DP  - 2015 Nov 13
TI  - Cartilage oligomeric matrix protein and matrix metalloproteinase-3 expression in 
      the serum and joint fluid of a reversible osteoarthritis rabbit model.
PG  - 14207-15
LID - 10.4238/2015.November.13.4 [doi]
AB  - The main pathological characteristic of osteoarthritis (OA) is cartilage damage. 
      We explored cartilage oligomeric matrix protein (COMP) and matrix 
      metalloproteinase-3 (MMP-3) changes during articular cartilage injury and repair. 
      Rabbits were randomly divided into the following: a blank control group; groups 
      M1, M2, and M3, in which breaking was performed for 2, 4, and 6 weeks, 
      respectively; and groups L1, L2, and L3, in which breaking was discontinued for 
      2, 4, and 6 weeks, respectively, following a 4-week recovery period. There are 7 
      rabbits in each group. The degree of cartilage damage in each group was scored 
      (OA score). An enzyme-linked immunosorbent assay was used to detect COMP and 
      MMP-3 levels in serum and joint fluid. The OA scores were 3.89 +/- 2.31, 7.21 +/- 
      2.31, and 10.88 +/- 2.08 points in groups M1, M2, and M3, respectively (P < 0.05). 
      COMP and MMP-3 levels were significantly higher in groups M1, M2, and M3 than in 
      C. The OA score improved significantly following the 4-week recovery period (P < 
      0.05). COMP and MMP-3 levels began to decrease as the time following 
      discontinuation of breaking increased, but were higher than in the control (P < 
      0.05). MMP- 3 and COMP levels were correlated with OA score (r > 0.7, P < 0.05). 
      COMP and MMP-3 levels were correlated between joint fluid and serum (r = 0.899, r 
      = 0.874, P < 0.05, respectively). Long-term joint breaking can cause articular 
      cartilage damage. Doing some activites after the process can promote self-repair 
      of articular cartilage. COMP and MMP-3 levels were associated with articular 
      cartilage destruction and repair.
FAU - Chu, X Q
AU  - Chu XQ
AD  - Department of Orthopaedics, Shandong Provincial Qianfoshan Hospital, Shandong 
      University, Jinan, China.
FAU - Wang, J J
AU  - Wang JJ
AD  - Department of Neurosurgery, Shandong Provincial Qianfoshan Hospital, Shandong 
      University, Jinan, China.
FAU - Dou, L D
AU  - Dou LD
AD  - Department of Orthopaedics, Shandong Provincial Qianfoshan Hospital, Shandong 
      University, Jinan, China.
FAU - Zhao, G
AU  - Zhao G
AD  - Department of Orthopaedics, Shandong Provincial Qianfoshan Hospital, Shandong 
      University, Jinan, China zhaoganglife88@126.com.
LA  - eng
PT  - Journal Article
DEP - 20151113
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - 0 (Biomarkers)
RN  - 0 (Cartilage Oligomeric Matrix Protein)
RN  - 0 (Glycoproteins)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
SB  - IM
MH  - Animals
MH  - Biomarkers/blood/metabolism
MH  - Cartilage Oligomeric Matrix Protein/blood/genetics/*metabolism
MH  - Cartilage, Articular/enzymology/metabolism/pathology
MH  - Disease Models, Animal
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Glycoproteins/blood/metabolism
MH  - Male
MH  - Matrix Metalloproteinase 3/*biosynthesis/blood/genetics
MH  - Osteoarthritis, Knee/blood/genetics
MH  - Rabbits
MH  - Synovial Fluid/enzymology/*metabolism
MH  - Transcriptome
EDAT- 2015/11/26 06:00
MHDA- 2016/09/30 06:00
CRDT- 2015/11/25 06:00
PHST- 2015/11/25 06:00 [entrez]
PHST- 2015/11/26 06:00 [pubmed]
PHST- 2016/09/30 06:00 [medline]
AID - gmr6220 [pii]
AID - 10.4238/2015.November.13.4 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Nov 13;14(4):14207-15. doi: 10.4238/2015.November.13.4.