PMID- 26600507
OWN - NLM
STAT- MEDLINE
DCOM- 20160902
LR  - 20161126
IS  - 1676-5680 (Electronic)
IS  - 1676-5680 (Linking)
VI  - 14
IP  - 4
DP  - 2015 Nov 19
TI  - Identification of the origin of marker chromosomes by two-color fluorescence in 
      situ hybridization and polymerase chain reaction in azoospermic patients.
PG  - 14488-95
LID - 10.4238/2015.November.18.11 [doi]
AB  - Y chromosomal microdeletions at the azoospermia factor locus and chromosome 
      abnormalities have been implicated as the major causes of idiopathic male 
      infertility. A marker chromosome is a structurally abnormal chromosome in which 
      no part can be identified by cytogenetics. In this study, to identify the origin 
      of the marker chromosomes and to perform a genetic diagnosis of patients with 
      azoospermia, two-color fluorescence in situ hybridization (FISH) and polymerase 
      chain reaction (PCR) techniques were carried out. The marker chromosomes for the 
      two patients with azoospermia originated in the Y chromosome; it was ascertained 
      that the karyotype of both patients was 46,X, ish del(Y)(q11)(DYZ3+, DXZ1-). The 
      combination of two-color FISH and PCR techniques is an important method for the 
      identification of the origin of marker chromosomes. Thus, genetic counseling and 
      a clear genetic diagnosis of patients with azoospermia before intracytoplasmic 
      sperm injection or other clinical managements are important.
FAU - Wei, C L
AU  - Wei CL
AD  - Key Laboratory of Epigenetics and Oncology, Research Center for Preclinical 
      Medicine, Sichuan Medical University, Luzhou, Sichuan Province, China.
FAU - Cheng, J L
AU  - Cheng JL
AD  - Key Laboratory of Epigenetics and Oncology, Research Center for Preclinical 
      Medicine, Sichuan Medical University, Luzhou, Sichuan Province, China.
FAU - Yang, W C
AU  - Yang WC
AD  - Key Laboratory of Epigenetics and Oncology, Research Center for Preclinical 
      Medicine, Sichuan Medical University, Luzhou, Sichuan Province, China.
FAU - Li, L Y
AU  - Li LY
AD  - Insistitute of Reproduction and Stem Cell Engineering, Xiangya School of 
      Medicine, Central South University, Changsha, Hunan Province, China.
FAU - Cheng, H C
AU  - Cheng HC
AD  - Insistitute of Reproduction and Stem Cell Engineering, Xiangya School of 
      Medicine, Central South University, Changsha, Hunan Province, China.
FAU - Fu, J J
AU  - Fu JJ
AD  - Key Laboratory of Epigenetics and Oncology, Research Center for Preclinical 
      Medicine, Sichuan Medical University, Luzhou, Sichuan Province, China 
      fujunjiang@hotmail.com.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151119
PL  - Brazil
TA  - Genet Mol Res
JT  - Genetics and molecular research : GMR
JID - 101169387
RN  - Male sterility due to Y-chromosome deletions
SB  - IM
MH  - Azoospermia/*diagnosis/genetics/pathology
MH  - *Chromosome Aberrations
MH  - Chromosome Deletion
MH  - Chromosomes, Human, Y/genetics
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Infertility, Male
MH  - Karyotype
MH  - Male
MH  - Polymerase Chain Reaction
MH  - Sex Chromosome Aberrations
MH  - Sex Chromosome Disorders of Sex Development/*diagnosis/genetics/pathology
EDAT- 2015/11/26 06:00
MHDA- 2016/09/03 06:00
CRDT- 2015/11/25 06:00
PHST- 2015/11/25 06:00 [entrez]
PHST- 2015/11/26 06:00 [pubmed]
PHST- 2016/09/03 06:00 [medline]
AID - gmr5919 [pii]
AID - 10.4238/2015.November.18.11 [doi]
PST - epublish
SO  - Genet Mol Res. 2015 Nov 19;14(4):14488-95. doi: 10.4238/2015.November.18.11.