PMID- 26602876
OWN - NLM
STAT- MEDLINE
DCOM- 20160601
LR  - 20181113
IS  - 1678-8060 (Electronic)
IS  - 0074-0276 (Print)
IS  - 0074-0276 (Linking)
VI  - 110
IP  - 8
DP  - 2015 Dec
TI  - Co-administration of plasmid-encoded granulocyte-macrophage colony-stimulating 
      factor increases human immunodeficiency virus-1 DNA vaccine-induced 
      polyfunctional CD4+ T-cell responses.
PG  - 1010-6
LID - S0074-02762015005025104 [pii]
LID - 10.1590/0074-02760150283 [doi]
AB  - T-cell based vaccines against human immunodeficiency virus (HIV) generate 
      specific responses that may limit both transmission and disease progression by 
      controlling viral load. Broad, polyfunctional, and cytotoxic CD4+T-cell responses 
      have been associated with control of simian immunodeficiency virus/HIV-1 
      replication, supporting the inclusion of CD4+ T-cell epitopes in vaccine 
      formulations. Plasmid-encoded granulocyte-macrophage colony-stimulating factor 
      (pGM-CSF) co-administration has been shown to induce potent CD4+ T-cell responses 
      and to promote accelerated priming and increased migration of antigen-specific 
      CD4+ T-cells. However, no study has shown whether co-immunisation with pGM-CSF 
      enhances the number of vaccine-induced polyfunctional CD4+ T-cells. Our group has 
      previously developed a DNA vaccine encoding conserved, multiple human leukocyte 
      antigen (HLA)-DR binding HIV-1 subtype B peptides, which elicited broad, 
      polyfunctional and long-lived CD4+ T-cell responses. Here, we show that pGM-CSF 
      co-immunisation improved both magnitude and quality of vaccine-induced T-cell 
      responses, particularly by increasing proliferating CD4+ T-cells that produce 
      simultaneously interferon-gamma, tumour necrosis factor-alpha and interleukin-2. Thus, we 
      believe that the use of pGM-CSF may be helpful for vaccine strategies focused on 
      the activation of anti-HIV CD4+ T-cell immunity.
FAU - Santana, Vinicius Canato
AU  - Santana VC
AD  - Divisao de Imunologia Clinica e Alergia, Faculdade de Medicina, Universidade de 
      Sao Paulo, Sao Paulo, SP, Brasil.
FAU - Almeida, Rafael Ribeiro
AU  - Almeida RR
AD  - Divisao de Imunologia Clinica e Alergia, Faculdade de Medicina, Universidade de 
      Sao Paulo, Sao Paulo, SP, Brasil.
FAU - Ribeiro, Susan Pereira
AU  - Ribeiro SP
AD  - Divisao de Imunologia Clinica e Alergia, Faculdade de Medicina, Universidade de 
      Sao Paulo, Sao Paulo, SP, Brasil.
FAU - Ferreira, Luis Carlos de Souza
AU  - Ferreira LC
AD  - Departamento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de 
      Sao Paulo, Sao Paulo, SP, Brasil.
FAU - Kalil, Jorge
AU  - Kalil J
AD  - Instituto de Investigacao em Imunologia, Instituto Nacional de Ciencia e 
      Tecnologia, Sao Paulo, SP, Brasil.
FAU - Rosa, Daniela Santoro
AU  - Rosa DS
AD  - Instituto de Investigacao em Imunologia, Instituto Nacional de Ciencia e 
      Tecnologia, Sao Paulo, SP, Brasil.
FAU - Cunha-Neto, Edecio
AU  - Cunha-Neto E
AD  - Divisao de Imunologia Clinica e Alergia, Faculdade de Medicina, Universidade de 
      Sao Paulo, Sao Paulo, SP, Brasil.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151124
PL  - Brazil
TA  - Mem Inst Oswaldo Cruz
JT  - Memorias do Instituto Oswaldo Cruz
JID - 7502619
RN  - 0 (AIDS Vaccines)
RN  - 0 (Adjuvants, Immunologic)
RN  - 0 (Antigens, Viral)
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Interleukin-2)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 0 (Vaccines, DNA)
RN  - 82115-62-6 (Interferon-gamma)
RN  - 83869-56-1 (Granulocyte-Macrophage Colony-Stimulating Factor)
SB  - IM
MH  - AIDS Vaccines/*immunology
MH  - Adjuvants, Immunologic/administration & dosage
MH  - Animals
MH  - Antigens, Viral/*immunology
MH  - CD4-Positive T-Lymphocytes/drug effects/*immunology
MH  - Cell Movement/drug effects/immunology
MH  - Conserved Sequence/immunology
MH  - Enzyme-Linked Immunospot Assay
MH  - Female
MH  - Flow Cytometry
MH  - Genetic Vectors
MH  - Granulocyte-Macrophage Colony-Stimulating Factor/*administration & 
      dosage/immunology
MH  - HIV Infections/prevention & control
MH  - HIV-1/*immunology
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - Immunity, Cellular/*immunology
MH  - Interferon-gamma/drug effects/metabolism
MH  - Interleukin-2/metabolism
MH  - Lymphocyte Activation/drug effects/immunology
MH  - Mice, Inbred BALB C
MH  - Plasmids
MH  - Protein Binding/immunology
MH  - Tumor Necrosis Factor-alpha/drug effects/metabolism
MH  - Vaccines, DNA/*immunology
PMC - PMC4708021
EDAT- 2015/11/26 06:00
MHDA- 2016/06/02 06:00
PMCR- 2015/12/01
CRDT- 2015/11/26 06:00
PHST- 2015/07/29 00:00 [received]
PHST- 2015/10/20 00:00 [accepted]
PHST- 2015/11/26 06:00 [entrez]
PHST- 2015/11/26 06:00 [pubmed]
PHST- 2016/06/02 06:00 [medline]
PHST- 2015/12/01 00:00 [pmc-release]
AID - S0074-02762015005025104 [pii]
AID - 10.1590/0074-02760150283 [doi]
PST - ppublish
SO  - Mem Inst Oswaldo Cruz. 2015 Dec;110(8):1010-6. doi: 10.1590/0074-02760150283. 
      Epub 2015 Nov 24.