PMID- 26603150 OWN - NLM STAT- MEDLINE DCOM- 20161026 LR - 20180816 IS - 1531-2267 (Electronic) IS - 1094-8341 (Linking) VI - 48 IP - 2 DP - 2016 Feb TI - Peripheral vascular reactivity and serum BDNF responses to aerobic training are impaired by the BDNF Val66Met polymorphism. PG - 116-23 LID - 10.1152/physiolgenomics.00086.2015 [doi] AB - Besides neuronal plasticity, the neurotrophin brain-derived neurotrophic factor (BDNF) is also important in vascular function. The BDNF has been associated with angiogenesis through its specific receptor tropomyosin-related kinase B (TrkB). Additionally, Val66Met polymorphism decreases activity-induced BDNF. Since BDNF and TrkB are expressed in vascular endothelial cells and aerobic exercise training can increase serum BDNF, this study aimed to test the hypotheses: 1) Serum BDNF levels modulate peripheral blood flow; 2) The Val66Met BDNF polymorphism impairs exercise training-induced vasodilation. We genotyped 304 healthy male volunteers (Val66Val, n = 221; Val66Met, n = 83) who underwent intense aerobic exercise training on a running track three times/wk for 4 mo. We evaluated pre- and post-exercise training serum BDNF and proBDNF concentration, heart rate (HR), mean blood pressure (MBP), forearm blood flow (FBF), and forearm vascular resistance (FVR). In the pre-exercise training, BDNF, proBDNF, BDNF/proBDNF ratio, FBF, and FVR were similar between genotypes. After exercise training, functional capacity (V̇o2 peak) increased and HR decreased similarly in both groups. Val66Val, but not Val66Met, increased BDNF (interaction, P = 0.04) and BDNF/proBDNF ratio (interaction, P < 0.001). Interestingly, FBF (interaction, P = 0.04) and the FVR (interaction, P = 0.01) responses during handgrip exercise (HG) improved in Val66Val compared with Val66Met, even with similar responses of HR and MBP. There were association between BDNF/proBDNF ratio and FBF (r = 0.64, P < 0.001) and FVR (r = -0.58, P < 0.001) during HG exercise. These results show that peripheral vascular reactivity and serum BDNF responses to exercise training are impaired by the BDNF Val66Met polymorphism and such responsiveness is associated with serum BDNF concentrations in healthy subjects. CI - Copyright (c) 2016 the American Physiological Society. FAU - Lemos, Jose R Jr AU - Lemos JR Jr AD - School of Physical Education, Military Police of Sao Paulo State, Sao Paulo, Brazil; Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; FAU - Alves, Cleber R AU - Alves CR AD - School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil; FAU - de Souza, Silvia B C AU - de Souza SB AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; FAU - Marsiglia, Julia D C AU - Marsiglia JD AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; FAU - Silva, Michelle S M AU - Silva MS AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; FAU - Pereira, Alexandre C AU - Pereira AC AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; FAU - Teixeira, Antonio L AU - Teixeira AL AD - Federal University of Minas Gerais, Minas Gerais, Brazil; and. FAU - Vieira, Erica L M AU - Vieira EL AD - Federal University of Minas Gerais, Minas Gerais, Brazil; and. FAU - Krieger, Jose E AU - Krieger JE AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; FAU - Negrao, Carlos E AU - Negrao CE AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil; FAU - Alves, Guilherme B AU - Alves GB AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; FAU - de Oliveira, Edilamar M AU - de Oliveira EM AD - School of Physical Education and Sport, University of Sao Paulo, Sao Paulo, Brazil; FAU - Bolani, Wladimir AU - Bolani W AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; FAU - Dias, Rodrigo G AU - Dias RG AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; FAU - Trombetta, Ivani C AU - Trombetta IC AD - Heart Institute (InCor), University of Sao Paulo Medical School, Sao Paulo, Brazil; Universidade Nove de Julho (UNINOVE), Sao Paulo, Brazil ivani.trombetta@gmail.com. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151124 PL - United States TA - Physiol Genomics JT - Physiological genomics JID - 9815683 RN - 0 (Brain-Derived Neurotrophic Factor) RN - 0 (Membrane Glycoproteins) RN - 7171WSG8A2 (BDNF protein, human) RN - AE28F7PNPL (Methionine) RN - EC 2.7.10.1 (Protein-Tyrosine Kinases) RN - EC 2.7.10.1 (Receptor, trkB) RN - EC 2.7.10.1 (tropomyosin-related kinase-B, human) RN - HG18B9YRS7 (Valine) SB - IM MH - Adult MH - Blood Flow Velocity MH - Blood Pressure MH - Brain-Derived Neurotrophic Factor/*blood/*genetics MH - *Exercise MH - Exercise Test MH - Forearm/blood supply MH - Genotype MH - Hand Strength MH - Healthy Volunteers MH - Heart Rate MH - Humans MH - Male MH - Membrane Glycoproteins/genetics MH - Methionine/genetics MH - *Polymorphism, Genetic MH - Protein-Tyrosine Kinases/genetics MH - Receptor, trkB MH - Valine/genetics MH - Young Adult OTO - NOTNLM OT - BDNF Val66Met polymorphism OT - exercise training OT - vascular reactivity EDAT- 2015/11/26 06:00 MHDA- 2016/10/27 06:00 CRDT- 2015/11/26 06:00 PHST- 2015/08/19 00:00 [received] PHST- 2015/11/13 00:00 [accepted] PHST- 2015/11/26 06:00 [entrez] PHST- 2015/11/26 06:00 [pubmed] PHST- 2016/10/27 06:00 [medline] AID - physiolgenomics.00086.2015 [pii] AID - 10.1152/physiolgenomics.00086.2015 [doi] PST - ppublish SO - Physiol Genomics. 2016 Feb;48(2):116-23. doi: 10.1152/physiolgenomics.00086.2015. Epub 2015 Nov 24.