PMID- 26606930
OWN - NLM
STAT- MEDLINE
DCOM- 20160808
LR  - 20231213
IS  - 1617-4623 (Electronic)
IS  - 1617-4623 (Linking)
VI  - 291
IP  - 2
DP  - 2016 Apr
TI  - Whole-transcriptome analysis of mouse adipose tissue in response to short-term 
      caloric restriction.
PG  - 831-47
LID - 10.1007/s00438-015-1150-3 [doi]
AB  - Caloric restriction (CR) has been shown to extend the lifespan of many species by 
      improving cellular function and organismal health. Additionally, fat reduction by 
      CR may play an important role in lengthening lifespan and preventing severe 
      age-related diseases. Interestingly, CR induced the greatest transcriptome change 
      in the epididymal fat of mice in our study. In this transcriptome analysis, we 
      identified and categorized 446 genes that correlated with CR level. We observed 
      down-regulation of several signaling pathways, including insulin/insulin-like 
      growth factor 1 (insulin/IGF-1), epidermal growth factor (EGF), transforming 
      growth factor beta (TGF-beta), and canonical wingless-type mouse mammary tumor virus 
      integration site (Wnt). Many genes related to structural features, including 
      extracellular matrix structure, cell adhesion, and the cytoskeleton, were 
      down-regulated, with a strong correlation to the degree of CR. Furthermore, genes 
      related to the cell cycle and adipogenesis were down-regulated. These biological 
      processes are well-identified targets of insulin/IGF-1, EGF, TGF-beta, and Wnt 
      signaling. In contrast, genes involved in specific metabolic processes, including 
      the tricarboxylic acid cycle and the electron transport chain were up-regulated. 
      We performed in silico analysis of the promoter sequences of CR-responsive genes 
      and identified two associated transcription factors, Paired-like homeodomain 2 
      (Pitx2) and Paired box gene 6 (Pax6). Our results suggest that strict regulation 
      of signaling pathways is critical for creating the optimal energy homeostasis to 
      extend lifespan.
FAU - Kim, Seung-Soo
AU  - Kim SS
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul, 136-701, Republic of Korea.
FAU - Choi, Kyung-Mi
AU  - Choi KM
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul, 136-701, Republic of Korea.
FAU - Kim, Soyoung
AU  - Kim S
AD  - Department of Food and Nutrition, Yonsei University, Seoul, 120-749, Republic of 
      Korea.
FAU - Park, Taesun
AU  - Park T
AD  - Department of Food and Nutrition, Yonsei University, Seoul, 120-749, Republic of 
      Korea.
FAU - Cho, In-Cheol
AU  - Cho IC
AD  - Subtropical Animal Station, National Institute of Animal Science, Jeju, 690-150, 
      Republic of Korea.
FAU - Lee, Jae-Won
AU  - Lee JW
AD  - Department of Statistics, Korea University, Seoul, 136-701, Republic of Korea.
FAU - Lee, Cheol-Koo
AU  - Lee CK
AD  - Department of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul, 136-701, Republic of Korea. cklee2005@korea.ac.kr.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151125
PL  - Germany
TA  - Mol Genet Genomics
JT  - Molecular genetics and genomics : MGG
JID - 101093320
RN  - 0 (Eye Proteins)
RN  - 0 (Homeodomain Proteins)
RN  - 0 (PAX6 Transcription Factor)
RN  - 0 (Paired Box Transcription Factors)
RN  - 0 (Pax6 protein, mouse)
RN  - 0 (Repressor Proteins)
RN  - 0 (Transcription Factors)
RN  - 0 (Transforming Growth Factor beta)
RN  - 62229-50-9 (Epidermal Growth Factor)
SB  - IM
MH  - Adipose Tissue/metabolism
MH  - Animals
MH  - *Caloric Restriction
MH  - Epidermal Growth Factor/biosynthesis
MH  - Eye Proteins/biosynthesis
MH  - Gene Expression Profiling/*methods
MH  - Gene Expression Regulation
MH  - Homeodomain Proteins/biosynthesis
MH  - Liver/metabolism
MH  - Longevity/*genetics
MH  - Mice
MH  - Oxidation-Reduction
MH  - PAX6 Transcription Factor
MH  - Paired Box Transcription Factors/biosynthesis
MH  - Repressor Proteins/biosynthesis
MH  - Transcription Factors/biosynthesis
MH  - Transcriptome/*genetics
MH  - Transforming Growth Factor beta/biosynthesis
MH  - Wnt Signaling Pathway
MH  - Homeobox Protein PITX2
OTO - NOTNLM
OT  - Adipose tissue
OT  - Caloric restriction strength
OT  - Mice
OT  - Transcription factor
OT  - Transcriptome
EDAT- 2015/11/27 06:00
MHDA- 2016/08/09 06:00
CRDT- 2015/11/27 06:00
PHST- 2015/05/09 00:00 [received]
PHST- 2015/11/16 00:00 [accepted]
PHST- 2015/11/27 06:00 [entrez]
PHST- 2015/11/27 06:00 [pubmed]
PHST- 2016/08/09 06:00 [medline]
AID - 10.1007/s00438-015-1150-3 [pii]
AID - 10.1007/s00438-015-1150-3 [doi]
PST - ppublish
SO  - Mol Genet Genomics. 2016 Apr;291(2):831-47. doi: 10.1007/s00438-015-1150-3. Epub 
      2015 Nov 25.