PMID- 26607090
OWN - NLM
STAT- MEDLINE
DCOM- 20160125
LR  - 20181202
IS  - 1673-4254 (Print)
IS  - 1673-4254 (Linking)
VI  - 35
IP  - 11
DP  - 2015 Nov
TI  - [Effects of tumor necrosis factor-alpha on release of MMP-3, MMP-9, and 
      interleukin-17 in mouse bone marrow-derived mast cells in vitro].
PG  - 1633-7
AB  - OBJECTIVE: To investigate the effect of tumor necrosis factor-alpha (TNF-alpha) on the 
      release of matrix metalloproteinase-3 (MMP-3), MMP-9, and interleukin-17 (IL-17) 
      in cultured mouse bone marrow-derived mast cells (BMMCs) in vitro. METHODS: 
      Primarily cultured mouse BMMCs at 8 weeks were exposed PBS (control) or TNF-alpha at 
      the concentrations of 2, 10, or 50 ng/mL for 12 or 24 h. Real-time PCR was 
      performed to detect the mRNA expressions of MMP-3, MMP-9, and IL-17 in the 
      exposed cells. RESULTS: A 12-hour exposure of the BMMCs to TNF-alpha caused 
      significantly increased expressions of MMP-3, MMP-9, and IL-17 in a 
      concentration-dependent manner (P<0.05). Prolonged exposures of the cells to 2 
      and 10 TNF-alpha for 24 h further increased MMP-3, MMP-9, and IL-17 mRNA expressions, 
      but exposure to 50 ng/mL TNF-alpha for 24 h increased only MMP-3 and MMP-9 
      expressions but not IL-17 mRNA expression. CONCLUSIONS: TNF-alpha treatment of 
      primarily cultured BMMCs can significantly increase the cellular expressions of 
      MMP-3, MMP-9, and IL-17 mRNA in a time- and dose-dependent manner.
FAU - Chen, Yujiao
AU  - Chen Y
AD  - Department of Rheumatology, Nanfang Hospital, Southern Medical University, 
      Guangzhou 510515, China.E-mail: 578320375@qq.com.
FAU - Ouyang, Qingqing
AU  - Ouyang Q
FAU - Wang, Ran
AU  - Wang R
FAU - Wu, Jing
AU  - Wu J
FAU - Zhao, Jinjun
AU  - Zhao J
FAU - Yang, Min
AU  - Yang M
LA  - chi
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Nan Fang Yi Ke Da Xue Xue Bao
JT  - Nan fang yi ke da xue xue bao = Journal of Southern Medical University
JID - 101266132
RN  - 0 (Interleukin-17)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - EC 3.4.24.17 (Matrix Metalloproteinase 3)
RN  - EC 3.4.24.17 (Mmp3 protein, mouse)
RN  - EC 3.4.24.35 (Matrix Metalloproteinase 9)
RN  - EC 3.4.24.35 (Mmp9 protein, mouse)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/metabolism
MH  - Cells, Cultured
MH  - Dose-Response Relationship, Drug
MH  - Interleukin-17/*metabolism
MH  - Mast Cells/*metabolism
MH  - Matrix Metalloproteinase 3/*metabolism
MH  - Matrix Metalloproteinase 9/*metabolism
MH  - Mice
MH  - RNA, Messenger
MH  - Real-Time Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/*pharmacology
EDAT- 2015/11/27 06:00
MHDA- 2016/01/26 06:00
CRDT- 2015/11/27 06:00
PHST- 2015/11/27 06:00 [entrez]
PHST- 2015/11/27 06:00 [pubmed]
PHST- 2016/01/26 06:00 [medline]
PST - ppublish
SO  - Nan Fang Yi Ke Da Xue Xue Bao. 2015 Nov;35(11):1633-7.