PMID- 26607208
OWN - NLM
STAT- MEDLINE
DCOM- 20170912
LR  - 20181202
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Linking)
VI  - 123
IP  - 6
DP  - 2015 Dec
TI  - Nociceptin/orphanin FQ up-regulates P2X(3) receptors in primary cultures of 
      neonatal rat trigeminal ganglion neurons.
PG  - 409-15
LID - 10.1111/eos.12228 [doi]
AB  - Recent evidence indicates a nociceptive role of the nociceptin/orphanin FQ-opioid 
      receptor-like receptor (N/OFQ-ORL1) system in craniofacial pain; however, the 
      mechanisms of such an effect remain unclear. We investigated whether the action 
      of N/OFQ involves the modulation of P2X3 , a pain-transducing ionotropic 
      receptor. Double-labeled immunohistochemical staining was used to determine the 
      co-localization of ORL1 and P2X3 receptors in the trigeminal ganglia (TG) of 
      neonatal Sprague-Dawley rats. The effect of N/OFQ (at a concentration ranging 
      from 1 pM to 10 nM) on the expression of P2X3 receptors was detected by RT-PCR, 
      western blotting, and immunocytochemical staining. We found that ORL1 receptors 
      were co-localized with P2X3 receptors and that the application of N/OFQ could 
      up-regulate, in a concentration-dependent manner, the expression of P2X3 receptor 
      mRNA and P2X3 receptor protein in TG neurons. Immunocytochemical staining also 
      revealed enhanced P2X3 immunoreactivity beneath the neuronal membrane and an 
      increased percentage of P2X3 -positive neurons after treatment with N/OFQ. Those 
      effects were completely blocked by a specific ORL1 antagonist, UFP-101. Our 
      results suggest that the activation of ORL1 receptors by N/OFQ can potentiate 
      P2X3 receptors in primary cultures of neonatal trigeminal neurons, which may be a 
      mechanism for the nociceptive role of N/OFQ in the modulation of craniofacial 
      pain. Our findings may also have implications in treating craniofacial pain.
CI  - (c) 2015 Eur J Oral Sci.
FAU - Wang, Yan
AU  - Wang Y
AD  - State Key Laboratory of Oral Diseases and Department of Orthodontics, West China 
      Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Long, Hu
AU  - Long H
AD  - State Key Laboratory of Oral Diseases and Department of Orthodontics, West China 
      Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Jian, Fan
AU  - Jian F
AD  - State Key Laboratory of Oral Diseases and Department of Orthodontics, West China 
      Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Li, Xiaoyu
AU  - Li X
AD  - State Key Laboratory of Oral Diseases, West China School of Stomatology, Sichuan 
      University, Chengdu, China.
FAU - Yang, Xin
AU  - Yang X
AD  - State Key Laboratory of Oral Diseases and Department of Orthodontics, West China 
      Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Zhao, Zhihe
AU  - Zhao Z
AD  - State Key Laboratory of Oral Diseases and Department of Orthodontics, West China 
      Hospital of Stomatology, Sichuan University, Chengdu, China.
FAU - Lai, Wenli
AU  - Lai W
AD  - State Key Laboratory of Oral Diseases and Department of Orthodontics, West China 
      Hospital of Stomatology, Sichuan University, Chengdu, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (RNA, Messenger)
RN  - 0 (Receptors, Purinergic P2X3)
SB  - IM
MH  - Animals
MH  - Neurons
MH  - RNA, Messenger
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Receptors, Purinergic P2X3
MH  - *Trigeminal Ganglion
OTO - NOTNLM
OT  - P2X3 receptor
OT  - craniofacial pain
OT  - nociceptin/orphanin FQ
OT  - opioid receptor-like receptor 1
OT  - trigeminal ganglion
EDAT- 2015/11/27 06:00
MHDA- 2017/09/13 06:00
CRDT- 2015/11/27 06:00
PHST- 2015/09/27 00:00 [accepted]
PHST- 2015/11/27 06:00 [entrez]
PHST- 2015/11/27 06:00 [pubmed]
PHST- 2017/09/13 06:00 [medline]
AID - 10.1111/eos.12228 [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2015 Dec;123(6):409-15. doi: 10.1111/eos.12228.