PMID- 26608536
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151215
LR  - 20200930
IS  - 1931-7573 (Print)
IS  - 1556-276X (Electronic)
IS  - 1556-276X (Linking)
VI  - 10
IP  - 1
DP  - 2015 Dec
TI  - Enhanced Antiproliferative Effect of Carboplatin in Cervical Cancer Cells 
      Utilizing Folate-Grafted Polymeric Nanoparticles.
PG  - 453
LID - 10.1186/s11671-015-1162-2 [doi]
LID - 453
AB  - Carboplatin (CRB) possesses superior anticancer effect in cervical cancer cells 
      with lower incidence of side effects compared to that of cisplatin. However, CRB 
      suffers from severe side effects due to undesirable tissue distributions which 
      contribute to the low therapeutic efficacy. Here, we report a unique folic 
      acid-conjugated chitosan-coated poly(D-L-lactideco-glycolide) (PLGA) 
      nanoparticles (FPCC) prepared for the selective delivery of carboplatin to the 
      cervical cancer cells. The particles were nanosized and spherical shaped with 
      size less than <200 nm. The presence of protective chitosan layer controlled the 
      overall release rate of CRB from chitosan-coated PLGA nanoparticles (PCC) and 
      FPCC. FPCC displayed a higher cellular uptake capacity in HeLa cells than 
      compared to non-targeted nanoparticles. Selective uptake of FPCC was due to an 
      interaction of folic acid (FA) with the folate receptors alpha (FRs-alpha) which is 
      overexpressed on the HeLa and promoted active targeting. These results indicated 
      that FPCC had a specific affinity for the cancerous, HeLa cells owing to 
      ligand-receptor (FA-FR-alpha) recognition. Consistently, FPCC showed superior 
      cytotoxic effect than any other formulations. The IC50 (concentration of the drug 
      required to kill 50 % of the cells) value of FPCC was 0.65 mug/ml while it was 
      1.08, 1.56, and 2.35 mug/ml for PCC, PLGA NP, and free CRB, respectively. 
      Consistent with the cytotoxicity assay, FPCC induced higher fraction of early as 
      well as late apoptosis cells. Especially, FPCC induced nearly 45 % of early 
      apoptosis cells and more than 35 % in late apoptosis. Therefore, we propose that 
      folate-conjugated nanoparticles might have potential applications in cervical 
      cancer therapy.
FAU - Ji, Jing
AU  - Ji J
AD  - Department of Gynecology & Obstetrics, The First Affiliated Hospital of Xi'An, 
      Jiaotong University, No. 277, Yanta Xi Road, Xian, Shanxi, 710061, China.
FAU - Zuo, Ping
AU  - Zuo P
AD  - Department of Gynecology & Obstetrics, The First Affiliated Hospital of Xi'An, 
      Jiaotong University, No. 277, Yanta Xi Road, Xian, Shanxi, 710061, China.
FAU - Wang, Yue-Ling
AU  - Wang YL
AD  - Department of Gynecology & Obstetrics, The First Affiliated Hospital of Xi'An, 
      Jiaotong University, No. 277, Yanta Xi Road, Xian, Shanxi, 710061, China. 
      wangyuelingwyl@hotmail.com.
LA  - eng
PT  - Journal Article
DEP - 20151125
PL  - United States
TA  - Nanoscale Res Lett
JT  - Nanoscale research letters
JID - 101279750
PMC - PMC4659799
OTO - NOTNLM
OT  - Apoptosis
OT  - Cervical cancers
OT  - Chitosan
OT  - Folate
OT  - PLGA nanoparticles
OT  - Targeting
EDAT- 2015/11/27 06:00
MHDA- 2015/11/27 06:01
PMCR- 2015/11/25
CRDT- 2015/11/27 06:00
PHST- 2015/07/29 00:00 [received]
PHST- 2015/11/15 00:00 [accepted]
PHST- 2015/11/27 06:00 [entrez]
PHST- 2015/11/27 06:00 [pubmed]
PHST- 2015/11/27 06:01 [medline]
PHST- 2015/11/25 00:00 [pmc-release]
AID - 10.1186/s11671-015-1162-2 [pii]
AID - 1162 [pii]
AID - 10.1186/s11671-015-1162-2 [doi]
PST - ppublish
SO  - Nanoscale Res Lett. 2015 Dec;10(1):453. doi: 10.1186/s11671-015-1162-2. Epub 2015 
      Nov 25.