PMID- 26608607
OWN - NLM
STAT- MEDLINE
DCOM- 20160526
LR  - 20190816
IS  - 0006-3002 (Print)
IS  - 0006-3002 (Linking)
VI  - 1863
IP  - 2
DP  - 2016 Feb
TI  - Post-transcriptional modulation of interleukin 8 by CNOT6L regulates skeletal 
      muscle differentiation.
PG  - 263-70
LID - S0167-4889(15)00402-4 [pii]
LID - 10.1016/j.bbamcr.2015.11.018 [doi]
AB  - CNOT6L is a deadenylase subunit belonging to the CCR4-NOT complex, a major 
      deadenylase complex in eukaryotes involved at multiple levels in regulation of 
      gene expression. While CNOT6L is expressed in skeletal muscle cells, its specific 
      functions in this tissue are still largely unknown. Our previous work highlighted 
      the functional of CNOT6L in skeletal muscle cell differentiation. To further 
      explore how CNOT6L regulates myogenesis, we used here gene expression analysis to 
      identify CNOT6L mRNA targets in human myoblasts. Among these novel targets, IL-8 
      (interleukin 8) mRNA was the most upregulated in CNOT6L knock-down (KD) cells. 
      Biochemical approaches and poly (A) tail length assays showed that IL-8 mRNA is a 
      direct target of CNOT6L, and further investigations by loss- and gain-of-function 
      assays pointed out that IL-8 is an important effector of myogenesis. Therefore, 
      we have characterized CNOT6L-IL-8 as a new signaling axis that regulates 
      myogenesis.
CI  - Copyright (c) 2015 Elsevier B.V. All rights reserved.
FAU - Polesskaya, Anna
AU  - Polesskaya A
AD  - Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ 
      Paris Saclay, Gif-sur-Yvette F-91198, France.
FAU - Pinna, Guillaume
AU  - Pinna G
AD  - Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ 
      Paris Saclay, Gif-sur-Yvette F-91198, France.
FAU - Sassi, Yassine
AU  - Sassi Y
AD  - Institute of Pharmacology and Toxicology, Technische Universitaet Muenchen (TUM), 
      Biedersteiner Strasse 29, 80802 Munich, Germany.
FAU - Vandamme, Marie
AU  - Vandamme M
AD  - Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ 
      Paris Saclay, Gif-sur-Yvette F-91198, France.
FAU - Bigot, Anne
AU  - Bigot A
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM UMRS974, CNRS FRE3617, Center 
      for Research in Myology, 47 Boulevard de l'hopital, 75013 Paris, France.
FAU - Mouly, Vincent
AU  - Mouly V
AD  - Sorbonne Universites, UPMC Univ Paris 06, INSERM UMRS974, CNRS FRE3617, Center 
      for Research in Myology, 47 Boulevard de l'hopital, 75013 Paris, France.
FAU - Morozova, Nadya
AU  - Morozova N
AD  - Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ 
      Paris Saclay, Gif-sur-Yvette F-91198, France.
FAU - Harel-Bellan, Annick
AU  - Harel-Bellan A
AD  - Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ 
      Paris Saclay, Gif-sur-Yvette F-91198, France.
FAU - Degerny, Cindy
AU  - Degerny C
AD  - Institute for Integrative Biology of the Cell (I2BC), IBiTecs, CEA, CNRS, Univ 
      Paris Saclay, Gif-sur-Yvette F-91198, France. Electronic address: 
      cindy.degerny@u-psud.fr.
LA  - eng
PT  - Journal Article
DEP - 20151201
PL  - Netherlands
TA  - Biochim Biophys Acta
JT  - Biochimica et biophysica acta
JID - 0217513
RN  - 0 (CXCL8 protein, human)
RN  - 0 (Interleukin-8)
RN  - 0 (RNA, Messenger)
RN  - EC 3.1.- (CNOT6L protein, human)
RN  - EC 3.1.- (Ribonucleases)
SB  - IM
MH  - Adult
MH  - Animals
MH  - Blotting, Western
MH  - Cell Differentiation/*genetics
MH  - Cell Line
MH  - Cells, Cultured
MH  - Gene Expression Profiling
MH  - Humans
MH  - Interleukin-8/*genetics/metabolism
MH  - Microscopy, Fluorescence
MH  - Muscle Development/genetics
MH  - Muscle Fibers, Skeletal/cytology/metabolism
MH  - Muscle, Skeletal/cytology/*metabolism
MH  - Myoblasts/cytology/metabolism
MH  - Oligonucleotide Array Sequence Analysis
MH  - RNA Interference
MH  - RNA, Messenger/genetics/metabolism
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Ribonucleases/*genetics/metabolism
MH  - Signal Transduction/genetics
MH  - Transcription, Genetic
OTO - NOTNLM
OT  - CNOT6L
OT  - Differentiation
OT  - IL-8
OT  - Myogenesis
OT  - Post-transcriptional regulation
EDAT- 2015/11/27 06:00
MHDA- 2016/05/27 06:00
CRDT- 2015/11/27 06:00
PHST- 2015/05/13 00:00 [received]
PHST- 2015/11/17 00:00 [revised]
PHST- 2015/11/18 00:00 [accepted]
PHST- 2015/11/27 06:00 [entrez]
PHST- 2015/11/27 06:00 [pubmed]
PHST- 2016/05/27 06:00 [medline]
AID - S0167-4889(15)00402-4 [pii]
AID - 10.1016/j.bbamcr.2015.11.018 [doi]
PST - ppublish
SO  - Biochim Biophys Acta. 2016 Feb;1863(2):263-70. doi: 10.1016/j.bbamcr.2015.11.018. 
      Epub 2015 Dec 1.