PMID- 26610206
OWN - NLM
STAT- MEDLINE
DCOM- 20160914
LR  - 20181113
IS  - 2041-4889 (Electronic)
VI  - 6
IP  - 11
DP  - 2015 Nov 26
TI  - A novel cyclic helix B peptide inhibits dendritic cell maturation during 
      amelioration of acute kidney graft rejection through Jak-2/STAT3/SOCS1.
PG  - e1993
LID - 10.1038/cddis.2015.338 [doi]
AB  - We recently synthesized a novel proteolysis-resistant cyclic helix B peptide 
      (CHBP) that exhibits promising renoprotective effects. Dendritic cells (DCs) play 
      an activation role in acute rejection (AR). Thus, the present study was designed 
      to investigate the effects of CHBP on DCs in a rat renal transplantation model. 
      The left kidney was harvested from male Lewis rats and then transplanted into 
      male Wistar rats with or without CHBP treatment. Five successive treatment doses 
      of CHBP after transplantation significantly ameliorated AR with lower 
      histological injury, apoptosis and CD4(+) and CD8(+) T-cell infiltration in renal 
      allografts. CHBP reduced IFN-gamma and IL-1beta levels but increased IL-4 and IL-10 
      levels in the serum. The number of mature DCs was significantly decreased in 
      renal allografts treated with CHBP. In addition, incubating DCs with CHBP in 
      vitro led to reduction in TNF-alpha, IFN-gamma, IL-1beta and IL-12 levels and increase of 
      IL-10 expression at the protein level in the supernatant. Mechanistically, CHBP 
      inhibited TLR activation-induced DC maturation by increasing SOCS1 expression 
      through Jak-2/STAT3 signaling. In conclusion, CHBP suppresses renal allograft AR 
      by inhibiting the maturation of DCs via Jak-2/STAT3/SOCS1 signaling, suggesting 
      that CHBP may be an potential therapeutic drug for treating renal AR.
FAU - Yang, C
AU  - Yang C
AD  - Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
AD  - Shanghai Key Laboratory of Organ Transplantation, Shanghai, China.
AD  - Department of Plastic Surgery, Zhongshan Hospital, Fudan University, Shanghai, 
      China.
FAU - Zhang, Y
AU  - Zhang Y
AD  - Shanghai Key Laboratory of Organ Transplantation, Shanghai, China.
AD  - Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai, 
      China.
FAU - Wang, J
AU  - Wang J
AD  - Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
AD  - Shanghai Key Laboratory of Organ Transplantation, Shanghai, China.
FAU - Li, L
AU  - Li L
AD  - Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
AD  - Shanghai Key Laboratory of Organ Transplantation, Shanghai, China.
FAU - Wang, L
AU  - Wang L
AD  - Biomedical Research Center, Zhongshan Hospital, Fudan University, Shanghai, 
      China.
FAU - Hu, M
AU  - Hu M
AD  - Shanghai Key Laboratory of Organ Transplantation, Shanghai, China.
FAU - Xu, M
AU  - Xu M
AD  - Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
AD  - Shanghai Key Laboratory of Organ Transplantation, Shanghai, China.
FAU - Long, Y
AU  - Long Y
AD  - CAS Key Laboratory of Receptor Research, Shanghai Institute of Materia Medica, 
      Chinese Academy of Sciences, Shanghai, China.
FAU - Rong, R
AU  - Rong R
AD  - Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
AD  - Shanghai Key Laboratory of Organ Transplantation, Shanghai, China.
AD  - Department of Transfusion, Zhongshan Hospital, Fudan University, Shanghai, China.
FAU - Zhu, T
AU  - Zhu T
AD  - Department of Urology, Zhongshan Hospital, Fudan University, Shanghai, China.
AD  - Shanghai Key Laboratory of Organ Transplantation, Shanghai, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151126
PL  - England
TA  - Cell Death Dis
JT  - Cell death & disease
JID - 101524092
RN  - 0 (Peptides, Cyclic)
RN  - 0 (STAT3 Transcription Factor)
RN  - EC 2.7.10.2 (JAK2 protein, human)
RN  - EC 2.7.10.2 (Janus Kinase 2)
SB  - IM
MH  - Animals
MH  - Cell Differentiation
MH  - Dendritic Cells/*drug effects
MH  - Graft Rejection/*genetics
MH  - Janus Kinase 2/*metabolism
MH  - Kidney Transplantation/*adverse effects
MH  - Peptides, Cyclic/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - STAT3 Transcription Factor/*metabolism
MH  - Signal Transduction
PMC - PMC4670942
EDAT- 2015/11/27 06:00
MHDA- 2016/09/15 06:00
PMCR- 2015/11/01
CRDT- 2015/11/27 06:00
PHST- 2015/07/16 00:00 [received]
PHST- 2015/09/22 00:00 [revised]
PHST- 2015/10/09 00:00 [accepted]
PHST- 2015/11/27 06:00 [entrez]
PHST- 2015/11/27 06:00 [pubmed]
PHST- 2016/09/15 06:00 [medline]
PHST- 2015/11/01 00:00 [pmc-release]
AID - cddis2015338 [pii]
AID - 10.1038/cddis.2015.338 [doi]
PST - epublish
SO  - Cell Death Dis. 2015 Nov 26;6(11):e1993. doi: 10.1038/cddis.2015.338.