PMID- 26612266
OWN - NLM
STAT- MEDLINE
DCOM- 20161103
LR  - 20191210
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 33
IP  - 3
DP  - 2016
TI  - Evaluation and validation of a multi-residue method based on biochip technology 
      for the simultaneous screening of six families of antibiotics in muscle and 
      aquaculture products.
PG  - 403-19
LID - 10.1080/19440049.2015.1125529 [doi]
AB  - The Evidence Investigator system (Randox, UK) is a biochip and semi-automated 
      system. The microarray kit II (AM II) is capable of detecting several compounds 
      belonging to different families of antibiotics: quinolones, ceftiofur, 
      thiamphenicol, streptomycin, tylosin and tetracyclines. The performance of this 
      innovative system was evaluated for the detection of antibiotic residues in new 
      matrices, in muscle of different animal species and in aquaculture products. The 
      method was validated according to the European Decision No. EC/2002/657 and the 
      European guideline for the validation of screening methods, which represents a 
      complete initial validation. The false-positive rate was equal to 0% in muscle 
      and in aquaculture products. The detection capabilities CCbeta for 12 validated 
      antibiotics (enrofloxacin, difloxacin, ceftiofur, desfuroyl ceftiofur cysteine 
      disulfide, thiamphenicol, florfenicol, tylosin, tilmicosin, streptomycin, 
      dihydrostreptomycin, tetracycline, doxycycline) were all lower than the 
      respective maximum residue limits (MRLs) in muscle from different animal origins 
      (bovine, ovine, porcine, poultry). No cross-reactions were observed with other 
      antibiotics, neither with the six detected families nor with other families of 
      antibiotics. The AM II kit could be applied to aquaculture products but with 
      higher detection capabilities from those in muscle. The detection capabilities 
      CCbeta in aquaculture products were respectively at 0.25, 0.10 and 0.5 of the 
      respective MRL in aquaculture products for enrofloxacin, tylosin and 
      oxytetracycline. The performance of the AM II kit has been compared with other 
      screening methods and with the performance characteristics previously determined 
      in honey.
FAU - Gaudin, Valerie
AU  - Gaudin V
AD  - a European Union Reference Laboratory, Anses Fougeres , La Haute Marche - BP 
      90203 , F-35302 Fougeres Cedex , France.
FAU - Hedou, Celine
AU  - Hedou C
AD  - a European Union Reference Laboratory, Anses Fougeres , La Haute Marche - BP 
      90203 , F-35302 Fougeres Cedex , France.
FAU - Soumet, Christophe
AU  - Soumet C
AD  - a European Union Reference Laboratory, Anses Fougeres , La Haute Marche - BP 
      90203 , F-35302 Fougeres Cedex , France.
FAU - Verdon, Eric
AU  - Verdon E
AD  - a European Union Reference Laboratory, Anses Fougeres , La Haute Marche - BP 
      90203 , F-35302 Fougeres Cedex , France.
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
PT  - Validation Study
DEP - 20160125
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Anti-Bacterial Agents)
SB  - IM
MH  - Animals
MH  - Anti-Bacterial Agents/*analysis/*classification
MH  - *Aquaculture
MH  - Automation
MH  - Cattle
MH  - Drug Residues/*analysis
MH  - Food Contamination/*analysis
MH  - Honey/analysis
MH  - *Microarray Analysis
MH  - Muscles/*chemistry
MH  - Salmon
MH  - Seafood/*analysis
MH  - Sheep
MH  - Swine
OTO - NOTNLM
OT  - European Union Decision No. 2002/657/EC
OT  - Validation
OT  - antibiotic
OT  - aquaculture
OT  - biochip
OT  - multi-array
OT  - muscle
OT  - screening
EDAT- 2015/11/28 06:00
MHDA- 2016/11/04 06:00
CRDT- 2015/11/28 06:00
PHST- 2015/11/28 06:00 [entrez]
PHST- 2015/11/28 06:00 [pubmed]
PHST- 2016/11/04 06:00 [medline]
AID - 10.1080/19440049.2015.1125529 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2016;33(3):403-19. 
      doi: 10.1080/19440049.2015.1125529. Epub 2016 Jan 25.