PMID- 26612455
OWN - NLM
STAT- MEDLINE
DCOM- 20161020
LR  - 20161230
IS  - 1878-3279 (Electronic)
IS  - 0171-2985 (Linking)
VI  - 221
IP  - 3
DP  - 2016 Mar
TI  - The molecular events behind ferulic acid mediated modulation of IL-6 expression 
      in LPS-activated Raw 264.7 cells.
PG  - 486-93
LID - S0171-2985(15)30085-1 [pii]
LID - 10.1016/j.imbio.2015.11.001 [doi]
AB  - Identification of new antioxidant and anti-inflammatory bioactive molecules is an 
      important tool for selecting effective formulations for the treatment of 
      inflammation. The mouse macrophage cell line RAW 264.7, lipopolysaccharide 
      (LPS)-activated, is associated with an inflammation response. Activated 
      macrophages produce reactive oxygen species (ROS), nitric oxide (NO) and 
      inflammatory cytokines such as IL-6, TNF-alpha and IL-10. In the present study we 
      have showed that pre-treatment with Ferulic Acid (FA) reduces NO accumulation in 
      the culture medium of LPS-induced macrophage cells. Moreover, real-time 
      experiments have revealed that FA has an inhibitory effect at the transcriptional 
      level on the expression of some inflammatory mediators such as IL-6, TNF-alpha and 
      iNOS and an activation effect on the expression of some antioxidant molecules 
      such as Metallothioneins (MT-1, MT-2). Importantly, we have found that FA reduced 
      the translocation of NF-E2-related factor 2 (Nrf2) and nuclear transcription 
      factor-kappaB (NF-kappaB) into the nuclei through a reduction of the expression of 
      phosphorylated IKK and consequently inhibited IL-6 and NF-kappaB promoter activity in 
      a luciferase assay. Our data clearly suggest that FA anti-inflammatory effects 
      are mainly mediated through IKK/NF-kappaB signalling pathway. Therefore, FA could 
      represent a new natural drug extremely useful to improve anti-inflammatory 
      treatment.
CI  - Copyright (c) 2015 Elsevier GmbH. All rights reserved.
FAU - Lampiasi, Nadia
AU  - Lampiasi N
AD  - Istituto di Biomedicina e Immunologia Molecolare "Alberto Monroy", Consiglio 
      Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy.
FAU - Montana, Giovanna
AU  - Montana G
AD  - Istituto di Biomedicina e Immunologia Molecolare "Alberto Monroy", Consiglio 
      Nazionale delle Ricerche, Via Ugo La Malfa 153, 90146 Palermo, Italy. Electronic 
      address: montana@ibim.cnr.it.
LA  - eng
PT  - Journal Article
DEP - 20151110
PL  - Netherlands
TA  - Immunobiology
JT  - Immunobiology
JID - 8002742
RN  - 0 (Antioxidants)
RN  - 0 (Coumaric Acids)
RN  - 0 (Interleukin-6)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Reactive Oxygen Species)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - AVM951ZWST (ferulic acid)
RN  - EC 2.7.11.10 (I-kappa B Kinase)
SB  - IM
MH  - Animals
MH  - Antioxidants/pharmacology
MH  - Cell Line
MH  - Coumaric Acids/*pharmacology
MH  - Gene Expression
MH  - Gene Expression Regulation/*drug effects
MH  - Genes, Reporter
MH  - I-kappa B Kinase/metabolism
MH  - Inflammation/genetics/immunology/metabolism
MH  - Interleukin-6/*genetics/*metabolism
MH  - Lipopolysaccharides/immunology
MH  - Macrophage Activation/*drug effects/*immunology
MH  - Macrophages/*drug effects/immunology/*metabolism
MH  - Mice
MH  - Models, Molecular
MH  - NF-kappa B/metabolism
MH  - Nitric Oxide/metabolism
MH  - Protein Transport
MH  - Reactive Oxygen Species/metabolism
OTO - NOTNLM
OT  - Ferulic Acid
OT  - IL-6
OT  - LPS
OT  - Metallothioneins
OT  - NF-kappaB
OT  - NO
OT  - Nrf2
OT  - ROS
OT  - iNOS
EDAT- 2015/11/28 06:00
MHDA- 2016/10/21 06:00
CRDT- 2015/11/28 06:00
PHST- 2015/09/24 00:00 [received]
PHST- 2015/10/30 00:00 [revised]
PHST- 2015/11/02 00:00 [accepted]
PHST- 2015/11/28 06:00 [entrez]
PHST- 2015/11/28 06:00 [pubmed]
PHST- 2016/10/21 06:00 [medline]
AID - S0171-2985(15)30085-1 [pii]
AID - 10.1016/j.imbio.2015.11.001 [doi]
PST - ppublish
SO  - Immunobiology. 2016 Mar;221(3):486-93. doi: 10.1016/j.imbio.2015.11.001. Epub 
      2015 Nov 10.