PMID- 26614261
OWN - NLM
STAT- MEDLINE
DCOM- 20161220
LR  - 20220321
IS  - 1573-2584 (Electronic)
IS  - 0301-1623 (Linking)
VI  - 48
IP  - 3
DP  - 2016 Mar
TI  - The effects of ozone therapy on caspase pathways, TNF-alpha, and HIF-1alpha in diabetic 
      nephropathy.
PG  - 441-50
LID - 10.1007/s11255-015-1169-8 [doi]
AB  - BACKGROUND: Accelerated apoptosis plays a vital role in the development of 
      diabetic vascular complications. Ozone may attenuate diabetic nephropathy by 
      means of decreased apoptosis-related genes. The aim of our study was to 
      investigate the effect of ozone therapy on streptozotocin-induced diabetic 
      nephropathy in rats. Also the histopathological changes in diabetic kidney tissue 
      with ozone treatment were evaluated. METHODS: The rats were randomly divided into 
      six groups (n = 7): control (C), ozone (O), diabetic (D), ozone-treated diabetic 
      (DO), insulin-treated diabetic (DI), and ozone- and insulin-treated diabetic 
      (DOI). D, DI, and DOI groups were induced by a single intraperitoneal injection 
      of streptozotocin. Ozone was given to the O, DO, and DOI groups. Group DI and DOI 
      received subcutaneous (SC) insulin (3 IU). All animals received daily treatment 
      for 6 weeks. RESULTS: Expressions of caspase-1-3-9, HIF-1alpha, and TNF-alpha genes were 
      significantly higher in D group compared to C group (p < 0.05 for all). Ozone 
      treatment resulted in significant decrease in the expressions of these genes in 
      diabetic kidney tissue compared to both C and D group (p < 0.05 for all). 
      Caspase-1-3-9, HIF-1alpha, and TNF-alpha gene expressions were found to be lower in DOI 
      group compared to C group (p < 0.05 for all). Also adding ozone treatment to 
      insulin therapy resulted in more significantly decrease in the expressions of 
      these genes in diabetic tissue compared to only insulin-treated diabetic group (p 
      < 0.05 for all). Regarding histological changes, ozone treatment resulted in 
      decrease in the renal corpuscular inflammation and normal kidney morphology was 
      observed. Both insulin and ozone therapies apparently improved kidney 
      histological findings with less degenerated tubules and less inflammation of 
      renal corpuscle compared to D, DO, and DI groups. CONCLUSION: Ozone therapy 
      decreases the expressions of apoptotic genes in diabetic kidney tissue and 
      improves the histopathological changes.
FAU - Guclu, Aydin
AU  - Guclu A
AD  - Department of Nephrology, Faculty of Medicine, Ahi Evran University, Kirsehir, 
      Turkey. aydinguclu@gmail.com.
FAU - Erken, Haydar Ali
AU  - Erken HA
AD  - Department of Physiology, Faculty of Medicine, Balikesir University, Balikesir, 
      Turkey.
FAU - Erken, Gulten
AU  - Erken G
AD  - Department of Physiology, Faculty of Medicine, Balikesir University, Balikesir, 
      Turkey.
FAU - Dodurga, Yavuz
AU  - Dodurga Y
AD  - Department of Medical Biology, Pamukkale University School of Medicine, Denizli, 
      Turkey.
FAU - Yay, Arzu
AU  - Yay A
AD  - Department of Histology and Embryology, Erciyes University School of Medicine, 
      Kayseri, Turkey.
FAU - Ozcoban, Ozge
AU  - Ozcoban O
AD  - Department of Histology and Embryology, Erciyes University School of Medicine, 
      Kayseri, Turkey.
FAU - Simsek, Hasan
AU  - Simsek H
AD  - Department of Physiology, Faculty of Medicine, Dumlupinar University, Kutahya, 
      Turkey.
FAU - Akcilar, Aydin
AU  - Akcilar A
AD  - Experimental Research Unit, Faculty of Medicine, Dumlupinar University, Kutahya, 
      Turkey.
FAU - Kocak, Fatma Emel
AU  - Kocak FE
AD  - Department of Biochemistry, Faculty of Medicine, Dumlupinar University, Kutahya, 
      Turkey.
LA  - eng
PT  - Journal Article
DEP - 20151127
PL  - Netherlands
TA  - Int Urol Nephrol
JT  - International urology and nephrology
JID - 0262521
RN  - 0 (Hif1a protein, rat)
RN  - 0 (Hypoxia-Inducible Factor 1, alpha Subunit)
RN  - 0 (Oxidants, Photochemical)
RN  - 0 (RNA, Messenger)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - 66H7ZZK23N (Ozone)
RN  - EC 3.4.22.- (Caspases)
SB  - IM
MH  - Animals
MH  - Apoptosis
MH  - Caspases/biosynthesis/*genetics
MH  - *Diabetes Mellitus, Experimental
MH  - Diabetic Nephropathies/*genetics/metabolism/therapy
MH  - *Gene Expression Regulation
MH  - Hypoxia-Inducible Factor 1, alpha Subunit/biosynthesis/*genetics
MH  - In Situ Nick-End Labeling
MH  - Male
MH  - Oxidants, Photochemical/therapeutic use
MH  - Ozone/*therapeutic use
MH  - RNA, Messenger/genetics
MH  - Rats
MH  - Rats, Sprague-Dawley
MH  - Real-Time Polymerase Chain Reaction
MH  - Tumor Necrosis Factor-alpha/biosynthesis/*genetics
OTO - NOTNLM
OT  - Caspase
OT  - Diabetic nephropathy
OT  - HIF-1alpha
OT  - Ozone therapy
OT  - TNF-alpha
EDAT- 2015/11/29 06:00
MHDA- 2016/12/21 06:00
CRDT- 2015/11/29 06:00
PHST- 2015/08/04 00:00 [received]
PHST- 2015/11/17 00:00 [accepted]
PHST- 2015/11/29 06:00 [entrez]
PHST- 2015/11/29 06:00 [pubmed]
PHST- 2016/12/21 06:00 [medline]
AID - 10.1007/s11255-015-1169-8 [pii]
AID - 10.1007/s11255-015-1169-8 [doi]
PST - ppublish
SO  - Int Urol Nephrol. 2016 Mar;48(3):441-50. doi: 10.1007/s11255-015-1169-8. Epub 
      2015 Nov 27.