PMID- 26614362
OWN - NLM
STAT- MEDLINE
DCOM- 20161230
LR  - 20181113
IS  - 1573-0832 (Electronic)
IS  - 0301-486X (Linking)
VI  - 181
IP  - 3-4
DP  - 2016 Apr
TI  - Possible Role of Trichophytin Antigen in Inducing Impaired Immunological 
      Clearance of Fungus in Onychomycosis.
PG  - 247-51
LID - 10.1007/s11046-015-9973-3 [doi]
AB  - The immunology of onychomycosis is poorly understood. Th1 and Th17 are the 
      principal effector cells responsible for protective immunity against fungi, while 
      it is assumed that Th2 responses are associated with deleterious effects. The 
      study was conducted to appraise the role of interleukin-6 (IL-6), transforming 
      growth factor beta (TGF-beta) and immunoglobulin E (IgE) in onychomycosis patients and 
      to study skin reactivity to trichophytin antigen in them. Serum samples of 60 
      cases of chronic onychomycosis and 30 healthy controls were assayed for serum 
      IgE, IL-6 and TGF-beta levels using specific immunoassay kits; 0.01 ml of 
      trichophytin antigen, Candida antigen and phosphate-buffered saline using 
      separate syringes were injected intradermal at three independent sites of the 
      forearm in cases and controls. Serum IL-6 levels were significantly lower in 
      cases as compared to controls, while serum TGF-beta levels in both cases and 
      controls were comparable. Serum IgE levels in cases were significantly higher 
      when compared with controls. Thirty-eight patients showed immediate 
      hypersensitivity response to trichophytin antigen, while none showed delayed 
      hypersensitivity reaction to trichophytin antigen. Constant fungal antigenic 
      stimuli induce a state of anergy as indicated by low serum IL-6 levels and the 
      absence of delayed hypersensitivity reaction to trichophytin antigen in cases, 
      leading to chronicity of infection. High total IgE may indicate a high 
      probability of prior fungal sensitization.
FAU - Gupta, Chhavi
AU  - Gupta C
AD  - Department of Microbiology, University College of Medical Sciences (UCMS) and 
      Guru Teg Bahadur Hospital (GTB Hospital), Dilshad Garden, Delhi, 110095, India. 
      chhavi13.86@gmail.com.
FAU - Das, Shukla
AU  - Das S
AD  - Department of Microbiology, University College of Medical Sciences (UCMS) and 
      Guru Teg Bahadur Hospital (GTB Hospital), Dilshad Garden, Delhi, 110095, India.
FAU - Ramachandran, V G
AU  - Ramachandran VG
AD  - Department of Microbiology, University College of Medical Sciences (UCMS) and 
      Guru Teg Bahadur Hospital (GTB Hospital), Dilshad Garden, Delhi, 110095, India.
FAU - Saha, Rumpa
AU  - Saha R
AD  - Department of Microbiology, University College of Medical Sciences (UCMS) and 
      Guru Teg Bahadur Hospital (GTB Hospital), Dilshad Garden, Delhi, 110095, India.
FAU - Bhattacharya, S N
AU  - Bhattacharya SN
AD  - Department of Dermatology, University College of Medical Sciences (UCMS) and Guru 
      Teg Bahadur Hospital (GTB Hospital), Dilshad Garden, Delhi, 110095, India.
FAU - Dar, Sajad Ahmad
AU  - Dar SA
AD  - Department of Microbiology, University College of Medical Sciences (UCMS) and 
      Guru Teg Bahadur Hospital (GTB Hospital), Dilshad Garden, Delhi, 110095, India.
AD  - Women's Health Services, Henry Ford Health System, 1 Ford Place, Detroit, MI, 
      48202, USA.
FAU - Atri, Dharmendra
AU  - Atri D
AD  - Department of Microbiology, University College of Medical Sciences (UCMS) and 
      Guru Teg Bahadur Hospital (GTB Hospital), Dilshad Garden, Delhi, 110095, India.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151127
PL  - Netherlands
TA  - Mycopathologia
JT  - Mycopathologia
JID - 7505689
RN  - 0 (Antigens, Fungal)
RN  - 0 (IL6 protein, human)
RN  - 0 (Interleukin-6)
RN  - 0 (Transforming Growth Factor beta)
RN  - 1404-86-0 (Trichophytin)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
EIN - Mycopathologia. 2016 Apr;181(3-4):253. PMID: 26821300
MH  - Adolescent
MH  - Adult
MH  - Aged
MH  - Antigens, Fungal/*immunology
MH  - Candida albicans/*immunology
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/*blood/immunology
MH  - Interleukin-6/*blood/immunology
MH  - Male
MH  - Middle Aged
MH  - Onychomycosis/*immunology/microbiology
MH  - Skin/immunology/microbiology/pathology
MH  - Transforming Growth Factor beta/*blood/immunology
MH  - Trichophytin/*immunology
MH  - Trichophyton/*immunology
MH  - Young Adult
OTO - NOTNLM
OT  - Cytokines
OT  - Dermatophytes
OT  - ELISA
OT  - Hypersensitivity
OT  - Onychomycosis
EDAT- 2015/11/29 06:00
MHDA- 2016/12/31 06:00
CRDT- 2015/11/29 06:00
PHST- 2015/06/18 00:00 [received]
PHST- 2015/11/21 00:00 [accepted]
PHST- 2015/11/29 06:00 [entrez]
PHST- 2015/11/29 06:00 [pubmed]
PHST- 2016/12/31 06:00 [medline]
AID - 10.1007/s11046-015-9973-3 [pii]
AID - 10.1007/s11046-015-9973-3 [doi]
PST - ppublish
SO  - Mycopathologia. 2016 Apr;181(3-4):247-51. doi: 10.1007/s11046-015-9973-3. Epub 
      2015 Nov 27.