PMID- 26617487
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20151130
LR  - 20200929
IS  - 1662-5102 (Print)
IS  - 1662-5102 (Electronic)
IS  - 1662-5102 (Linking)
VI  - 9
DP  - 2015
TI  - Use of Genetically Encoded Calcium Indicators (GECIs) Combined with Advanced 
      Motion Tracking Techniques to Examine the Behavior of Neurons and Glia in the 
      Enteric Nervous System of the Intact Murine Colon.
PG  - 436
LID - 10.3389/fncel.2015.00436 [doi]
LID - 436
AB  - Genetically encoded Ca(2+) indicators (GECIs) have been used extensively in many 
      body systems to detect Ca(2+) transients associated with neuronal activity. Their 
      adoption in enteric neurobiology has been slower, although they offer many 
      advantages in terms of selectivity, signal-to-noise and non-invasiveness. Our 
      aims were to utilize a number of cell-specific promoters to express the Ca(2+) 
      indicator GCaMP3 in different classes of neurons and glia to determine their 
      effectiveness in measuring activity in enteric neural networks during colonic 
      motor behaviors. We bred several GCaMP3 mice: (1) Wnt1-GCaMP3, all enteric 
      neurons and glia; (2) GFAP-GCaMP3, enteric glia; (3) nNOS-GaMP3, enteric 
      nitrergic neurons; and (4) ChAT-GCaMP3, enteric cholinergic neurons. These mice 
      allowed us to study the behavior of the enteric neurons in the intact colon 
      maintained at a physiological temperature, especially during the colonic 
      migrating motor complex (CMMC), using low power Ca(2+) imaging. In this 
      preliminary study, we observed neuronal and glial cell Ca(2+) transients in 
      specific cells in both the myenteric and submucous plexus in all of the 
      transgenic mice variants. The number of cells that could be simultaneously imaged 
      at low power (100-1000 active cells) through the undissected gut required 
      advanced motion tracking and analysis routines. The pattern of Ca(2+) transients 
      in myenteric neurons showed significant differences in response to spontaneous, 
      oral or anal stimulation. Brief anal elongation or mucosal stimulation, which 
      evokes a CMMC, were the most effective stimuli and elicited a powerful 
      synchronized and prolonged burst of Ca(2+) transients in many myenteric neurons, 
      especially when compared with the same neurons during a spontaneous CMMC. In 
      contrast, oral elongation, which normally inhibits CMMCs, appeared to suppress 
      Ca(2+) transients in some of the neurons active during a spontaneous or an anally 
      evoked CMMC. The activity in glial networks appeared to follow neural activity 
      but continued long after neural activity had waned. With these new tools an 
      unprecedented level of detail can be recorded from the enteric nervous system 
      (ENS) with minimal manipulation of tissue. These techniques can be extended in 
      order to better understand the roles of particular enteric neurons and glia 
      during normal and disordered motility.
FAU - Hennig, Grant W
AU  - Hennig GW
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine Reno, NV, USA.
FAU - Gould, Thomas W
AU  - Gould TW
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine Reno, NV, USA.
FAU - Koh, Sang Don
AU  - Koh SD
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine Reno, NV, USA.
FAU - Corrigan, Robert D
AU  - Corrigan RD
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine Reno, NV, USA.
FAU - Heredia, Dante J
AU  - Heredia DJ
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine Reno, NV, USA.
FAU - Shonnard, Matthew C
AU  - Shonnard MC
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine Reno, NV, USA.
FAU - Smith, Terence K
AU  - Smith TK
AD  - Department of Physiology and Cell Biology, University of Nevada School of 
      Medicine Reno, NV, USA.
LA  - eng
GR  - P01 DK041315/DK/NIDDK NIH HHS/United States
GR  - P20 GM103554/GM/NIGMS NIH HHS/United States
GR  - P20 RR018751/RR/NCRR NIH HHS/United States
PT  - Journal Article
DEP - 20151110
PL  - Switzerland
TA  - Front Cell Neurosci
JT  - Frontiers in cellular neuroscience
JID - 101477935
PMC - PMC4639702
OTO - NOTNLM
OT  - CMMC
OT  - GECIs
OT  - calcium
OT  - colon
OT  - myenteric plexus
OT  - submucous plexus
EDAT- 2015/12/01 06:00
MHDA- 2015/12/01 06:01
PMCR- 2015/01/01
CRDT- 2015/12/01 06:00
PHST- 2015/09/08 00:00 [received]
PHST- 2015/10/16 00:00 [accepted]
PHST- 2015/12/01 06:00 [entrez]
PHST- 2015/12/01 06:00 [pubmed]
PHST- 2015/12/01 06:01 [medline]
PHST- 2015/01/01 00:00 [pmc-release]
AID - 10.3389/fncel.2015.00436 [doi]
PST - epublish
SO  - Front Cell Neurosci. 2015 Nov 10;9:436. doi: 10.3389/fncel.2015.00436. 
      eCollection 2015.