PMID- 26617795
OWN - NLM
STAT- MEDLINE
DCOM- 20160926
LR  - 20211203
IS  - 1936-2625 (Electronic)
IS  - 1936-2625 (Linking)
VI  - 8
IP  - 9
DP  - 2015
TI  - TMP21 modulates cell growth in papillary thyroid cancer cells by inducing 
      autophagy through activation of the AMPK/mTOR pathway.
PG  - 10824-31
AB  - OBJECTIVE: To investigate the role of transmembrane protein (TMP) 21 in human 
      thyroid cancer. METHODS: The recombinant expression vector pcDNA3.1 (+)-TMP21 and 
      specific small interfering RNAs (siRNA) against TMP21 were transfected into a 
      papillary thyroid cancer cell line (TPC1). After transfection, the expression of 
      TMP21 was confirmed by quantitative real-time polymerase chain reaction (qRT-PCR) 
      and Western blotting. Moreover, cell viability and apoptosis rate were 
      respectively determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium 
      bromide (MTT) colorimetric assay and flow cytometry (FCM). Additionally, Western 
      blotting was performed to analyze the adenosine monophosphate (AMP)-activated 
      protein kinase (AMPK)/mammalian target of rapamycin (mTOR) pathways associated 
      protein (P-AMPKalpha(Thr172), P-mTOR(Ser2448), light chain (LC)-II/LC3-I, and 
      P-S6K(Thr389)) after pre-treatment with AMPK inhibitor, compound C (Com C) and 
      siTMP21. RESULTS: The TMP21 protein level and cell viability were significantly 
      higher, but apoptotic rate was significantly lower by transfection with 
      pcDNA3.1-TMP21 than those in control group (P < 0.05), and reverse results were 
      obtained by transfection with siTMP21. However, qRT-PCR showed different results 
      due to the feedback inhibition of mRNA. Besides, silencing of TMP21 significantly 
      reduced the levels of P-mTOR(Ser2448) and P-S6K(Thr389) (P < 0.05), but 
      significantly increased the levels of P-AMPKalpha(Thr172) and LC3-II/LC3-I compared 
      with the control group (P < 0.01). Whereas, the levels of P-AMPKalpha(Thr172) and 
      LC3-II/LC3-I were significantly decreased by Com C compared with the control 
      group (P < 0.05). CONCLUSION: TMP21 modulates cell growth in TPC1 cells by 
      inducing autophagy, which may be associated with activation of AMPK/mTOR pathway.
FAU - Xu, Xiaobo
AU  - Xu X
AD  - Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai 
      JiaoTong University School of Medicine Shanghai 200011, China.
FAU - Gao, Hongqiang
AU  - Gao H
AD  - Department of General Surgery, Shanghai Fengcheng Hospital Shanghai 201411, 
      China.
FAU - Qin, Jian
AU  - Qin J
AD  - Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai 
      JiaoTong University School of Medicine Shanghai 200011, China.
FAU - He, Liu
AU  - He L
AD  - Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai 
      JiaoTong University School of Medicine Shanghai 200011, China.
FAU - Liu, Wenyong
AU  - Liu W
AD  - Department of General Surgery, Shanghai Ninth People's Hospital, Shanghai 
      JiaoTong University School of Medicine Shanghai 200011, China.
LA  - eng
PT  - Journal Article
DEP - 20150901
PL  - United States
TA  - Int J Clin Exp Pathol
JT  - International journal of clinical and experimental pathology
JID - 101480565
RN  - 0 (Membrane Proteins)
RN  - 0 (Nucleocytoplasmic Transport Proteins)
RN  - 0 (Protein Kinase Inhibitors)
RN  - 0 (TMED10 protein, human)
RN  - EC 2.7.1.1 (MTOR protein, human)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - EC 2.7.11.31 (AMP-Activated Protein Kinases)
SB  - IM
MH  - AMP-Activated Protein Kinases/antagonists & inhibitors/*metabolism
MH  - Apoptosis
MH  - *Autophagy/drug effects
MH  - Carcinoma/*enzymology/genetics/pathology
MH  - Carcinoma, Papillary
MH  - Cell Line, Tumor
MH  - *Cell Proliferation/drug effects
MH  - Cell Survival
MH  - Enzyme Activation
MH  - Gene Expression Regulation, Neoplastic
MH  - Humans
MH  - Membrane Proteins/genetics/*metabolism
MH  - Nucleocytoplasmic Transport Proteins
MH  - Phosphorylation
MH  - Protein Kinase Inhibitors/pharmacology
MH  - RNA Interference
MH  - Signal Transduction
MH  - TOR Serine-Threonine Kinases/*metabolism
MH  - Thyroid Cancer, Papillary
MH  - Thyroid Neoplasms/*enzymology/genetics/pathology
MH  - Time Factors
MH  - Transfection
PMC - PMC4637610
OTO - NOTNLM
OT  - AMPK/mTOR
OT  - Thyroid cancer
OT  - autophagy
OT  - transmembrane protein 21
EDAT- 2015/12/01 06:00
MHDA- 2016/09/27 06:00
PMCR- 2015/09/01
CRDT- 2015/12/01 06:00
PHST- 2015/05/25 00:00 [received]
PHST- 2015/06/29 00:00 [accepted]
PHST- 2015/12/01 06:00 [entrez]
PHST- 2015/12/01 06:00 [pubmed]
PHST- 2016/09/27 06:00 [medline]
PHST- 2015/09/01 00:00 [pmc-release]
PST - epublish
SO  - Int J Clin Exp Pathol. 2015 Sep 1;8(9):10824-31. eCollection 2015.