PMID- 26620841 OWN - NLM STAT- MEDLINE DCOM- 20161012 LR - 20181202 IS - 1873-2518 (Electronic) IS - 0264-410X (Linking) VI - 34 IP - 3 DP - 2016 Jan 12 TI - Prediction and identification of novel IBV S1 protein derived CTL epitopes in chicken. PG - 380-6 LID - S0264-410X(15)01684-9 [pii] LID - 10.1016/j.vaccine.2015.11.042 [doi] AB - Infectious bronchitis virus (IBV) is a major pathogen common in the poultry industry. Broad cytotoxic T lymphocyte (CTL) response against IBV is one of the crucial factors that help to control viral replication. Spike glycoproteins on the surface of the IBV virion harbor major T cell epitopes. In this study, based on the peptide-binding motifs of chicken MHC I molecules for the BF2*4, BF2*12, BF2*15, and BF2*19 haplotypes, potential CTL epitopes were predicted using S1 proteins from different IBV strains. Twenty-one peptides were predicted to be potential CTL epitopes; they were manually synthesized and the CTL responses to them tested in vitro. Spleen lymphocytes were collected from specific-pathogen free (SPF) chicken that had been immunized with the S1 protein expression plasmid, pV-S1, and were stimulated by the synthesized peptides. IFN-gamma secretion and CD8(+) T cell proliferation in chickens were tested by ELISpot array and flow cytometry, respectively. Four epitopes (P8SRIQTATDP, P9SRNATGSQP, P18GAYAVVNV, and P19SRIQTATQP) were identified to stimulate CD8(+) T cell proliferation and IFN-gamma secretion, indicating their efficacy as CTL epitopes in chicken. Poly-CTL-epitope DNA vaccine (pV-S1T) was constructed by inserting nucleotide sequences encoding the P8, P9, P18, and P19 CTL epitopes into the pVAX1 vector. Chickens were vaccinated with either pV-S1, pV-S1T, or pVAX1 and the protection efficacy was analyzed, revealing that ninety percent of chickens immunized with pV-S1T were protected after challenge with 10(6) ELD50 of IBV, demonstrating that these novel CTL epitopes were effective against IBV challenge. This study provides a new method to screen virus CTL epitopes in chicken and to develop poly-CTL-epitope DNA vaccines. CI - Copyright (c) 2015 Elsevier Ltd. All rights reserved. FAU - Tan, Lei AU - Tan L AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. FAU - Liao, Ying AU - Liao Y AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. FAU - Fan, Jin AU - Fan J AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. FAU - Zhang, Yuqiang AU - Zhang Y AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. FAU - Mao, Xiang AU - Mao X AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. FAU - Sun, Yingjie AU - Sun Y AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. FAU - Song, Cuiping AU - Song C AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. FAU - Qiu, Xusheng AU - Qiu X AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. FAU - Meng, Chunchun AU - Meng C AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China. FAU - Ding, Chan AU - Ding C AD - Department of Avian Diseases, Shanghai Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Shanghai 200241, PR China; Jiangsu Co-innovation Center for Prevention and Control of Important Animal Infectious Diseases and Zoonoses, Yangzhou 225009, PR China. Electronic address: shoveldeen@shvri.ac.cn. LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't DEP - 20151125 PL - Netherlands TA - Vaccine JT - Vaccine JID - 8406899 RN - 0 (Epitopes, T-Lymphocyte) RN - 0 (Histocompatibility Antigens Class I) RN - 0 (Recombinant Fusion Proteins) RN - 0 (Spike Glycoprotein, Coronavirus) RN - 0 (Vaccines, DNA) RN - 0 (Vaccines, Synthetic) RN - 0 (Viral Vaccines) RN - 82115-62-6 (Interferon-gamma) SB - IM MH - Animals MH - Cell Proliferation MH - Chickens MH - Coronavirus Infections/prevention & control/veterinary MH - Enzyme-Linked Immunospot Assay MH - Epitopes, T-Lymphocyte/genetics/*immunology MH - Haplotypes MH - Histocompatibility Antigens Class I/genetics/metabolism MH - Infectious bronchitis virus/genetics/*immunology MH - Interferon-gamma/metabolism MH - Leukocytes, Mononuclear/immunology MH - Protein Binding MH - Recombinant Fusion Proteins/genetics/immunology MH - Spike Glycoprotein, Coronavirus/genetics/*immunology MH - Spleen/immunology MH - Survival Analysis MH - T-Lymphocytes, Cytotoxic/*immunology MH - Vaccines, DNA/administration & dosage/genetics/immunology MH - Vaccines, Synthetic/administration & dosage/genetics/immunology MH - Viral Vaccines/administration & dosage/genetics/immunology OTO - NOTNLM OT - Cytotoxic T lymphocyte OT - Epitopes OT - Haplotype OT - Immune protection OT - Infectious bronchitis virus OT - MHC I EDAT- 2015/12/02 06:00 MHDA- 2016/10/13 06:00 CRDT- 2015/12/02 06:00 PHST- 2015/07/08 00:00 [received] PHST- 2015/10/30 00:00 [revised] PHST- 2015/11/12 00:00 [accepted] PHST- 2015/12/02 06:00 [entrez] PHST- 2015/12/02 06:00 [pubmed] PHST- 2016/10/13 06:00 [medline] AID - S0264-410X(15)01684-9 [pii] AID - 10.1016/j.vaccine.2015.11.042 [doi] PST - ppublish SO - Vaccine. 2016 Jan 12;34(3):380-6. doi: 10.1016/j.vaccine.2015.11.042. Epub 2015 Nov 25.