PMID- 26621244
OWN - NLM
STAT- MEDLINE
DCOM- 20171215
LR  - 20180625
IS  - 1096-1186 (Electronic)
IS  - 1043-6618 (Linking)
VI  - 113
IP  - Pt B
DP  - 2016 Nov
TI  - The neuroprotective effects of R-phenibut after focal cerebral ischemia.
PG  - 796-801
LID - S1043-6618(15)30122-5 [pii]
LID - 10.1016/j.phrs.2015.11.013 [doi]
AB  - R-phenibut is a gamma-aminobutyric acid (GABA)-B receptor and alpha(2)-delta subunit of the 
      voltage-dependent calcium channel (VDCC) ligand. The aim of the present study was 
      to test the effects of R-phenibut on the motor, sensory and tactile functions and 
      histological outcomes in rats following transient middle cerebral artery 
      occlusion (MCAO). In this study, MCAO was induced by filament insertion (f-MCAO) 
      or endothelin-1 (ET1) microinjection (ET1-MCAO) in male Wistar or CD rats, 
      respectively. R-phenibut was administrated at doses of 10 and 50mg/kg for 14 days 
      in the f-MCAO or 7 days in the ET1-MCAO. The vibrissae-evoked forelimb-placing 
      and limb-placing tests were used to assess sensorimotor, tactile and 
      proprioceptive function. Quantitative reverse transcriptase-PCR was used to 
      detect brain-derived neurotrophic factor (BDNF) and vascular endothelial growth 
      factor (VEGF) gene expression in the damaged brain hemisphere. Both f-MCAO and 
      ET1-MCAO resulted in statistically significant impairment of sensorimotor 
      function and brain infarction. R-phenibut at a dose of 10mg/kg significantly 
      improved histological outcome at day 7 in the ET1-MCAO. R-phenibut treatment at a 
      dose of 50mg/kg significantly alleviated reduction of brain volume in damaged 
      hemisphere in both f-MCAO and ET1-MCAO. In R-phenibut treated animals a trend of 
      recovery of tactile and proprioceptive stimulation in the vibrissae-evoked 
      forelimb-placing test was observed. After R-phenibut treatment at a dose of 
      50mg/kg statistically significant increase of BDNF and VEGF gene expression was 
      found in damaged brain hemisphere. Taken together, obtained results provide 
      evidence for the neuroprotective activity of R-phenibut in experimental models of 
      stroke. These effects might be related to the modulatory effects of the drug on 
      the GABA-B receptor and alpha(2)-delta subunit of VDCC.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Vavers, Edijs
AU  - Vavers E
AD  - Latvian Institute of Organic Synthesis, Riga, Latvia; Riga Stradins University, 
      Riga, Latvia. Electronic address: edijs@biomed.lu.lv.
FAU - Zvejniece, Liga
AU  - Zvejniece L
AD  - Latvian Institute of Organic Synthesis, Riga, Latvia.
FAU - Svalbe, Baiba
AU  - Svalbe B
AD  - Latvian Institute of Organic Synthesis, Riga, Latvia.
FAU - Volska, Kristine
AU  - Volska K
AD  - Latvian Institute of Organic Synthesis, Riga, Latvia; Riga Stradins University, 
      Riga, Latvia.
FAU - Makarova, Elina
AU  - Makarova E
AD  - Latvian Institute of Organic Synthesis, Riga, Latvia.
FAU - Liepinsh, Edgars
AU  - Liepinsh E
AD  - Latvian Institute of Organic Synthesis, Riga, Latvia.
FAU - Rizhanova, Kristina
AU  - Rizhanova K
AD  - JSC Olainfarm, Olaine, Latvia.
FAU - Liepins, Vilnis
AU  - Liepins V
AD  - JSC Olainfarm, Olaine, Latvia.
FAU - Dambrova, Maija
AU  - Dambrova M
AD  - Latvian Institute of Organic Synthesis, Riga, Latvia; Riga Stradins University, 
      Riga, Latvia.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151124
PL  - Netherlands
TA  - Pharmacol Res
JT  - Pharmacological research
JID - 8907422
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Calcium Channels)
RN  - 0 (Eye Proteins)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (R-photopsin)
RN  - 0 (Receptors, GABA-B)
RN  - 0 (Vascular Endothelial Growth Factor A)
SB  - IM
MH  - Animals
MH  - Brain Ischemia/*drug therapy/metabolism
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Calcium Channels/metabolism
MH  - Disease Models, Animal
MH  - Eye Proteins/*pharmacology
MH  - Forelimb/drug effects/metabolism
MH  - Infarction, Middle Cerebral Artery/drug therapy/metabolism
MH  - Male
MH  - Neuroprotective Agents/*pharmacology
MH  - Rats
MH  - Rats, Wistar
MH  - Receptors, GABA-B/metabolism
MH  - Sensorimotor Cortex/*drug effects/metabolism
MH  - Stroke/drug therapy/metabolism
MH  - Vascular Endothelial Growth Factor A/metabolism
OTO - NOTNLM
OT  - BDNF
OT  - GABA-B receptors
OT  - R-phenibut
OT  - Stroke
OT  - VEGF
OT  - alpha(2)-delta subunit of VDCC
EDAT- 2015/12/02 06:00
MHDA- 2017/12/16 06:00
CRDT- 2015/12/02 06:00
PHST- 2015/10/21 00:00 [received]
PHST- 2015/11/19 00:00 [revised]
PHST- 2015/11/20 00:00 [accepted]
PHST- 2015/12/02 06:00 [pubmed]
PHST- 2017/12/16 06:00 [medline]
PHST- 2015/12/02 06:00 [entrez]
AID - S1043-6618(15)30122-5 [pii]
AID - 10.1016/j.phrs.2015.11.013 [doi]
PST - ppublish
SO  - Pharmacol Res. 2016 Nov;113(Pt B):796-801. doi: 10.1016/j.phrs.2015.11.013. Epub 
      2015 Nov 24.