PMID- 26621757
OWN - NLM
STAT- MEDLINE
DCOM- 20170201
LR  - 20170201
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 48
DP  - 2016 Jan
TI  - Molecular characterization and functional analysis of tumor necrosis factor 
      receptor-associated factor 2 in the Pacific oyster.
PG  - 12-9
LID - S1050-4648(15)30246-1 [pii]
LID - 10.1016/j.fsi.2015.11.027 [doi]
AB  - Tumor necrosis factor receptor (TNFR)-associated factors (TRAFs) are a family of 
      crucial adaptors, playing vital roles in mediating signal transduction in immune 
      signaling pathways, including RIG-I-like receptor (RLR) signaling pathway. In the 
      present study, a new TRAF family member (CgTRAF2) was identified in the Pacific 
      oyster, Crassostrea gigas. Comparison and phylogenetic analysis revealed that 
      CgTRAF2 could be a new member of the invertebrate TRAF2 family. Quantitative 
      real-time PCR revealed that CgTRAF2 mRNA was highly expressed in the digestive 
      gland, gills, and hemocytes, and it was significantly up-regulated after Vibrio 
      alginolyticus and ostreid herpesvirus 1 (OsHV-1) challenge. The CgTRAF2 mRNA 
      expression profile in different developmental stages of oyster larvae suggested 
      that CgTRAF2 could function in early larval development. CgTRAF2 mRNA expression 
      pattern, after the silence of CgMAVS (Mitochondrial Antiviral Signaling) -like, 
      indicated that CgTRAF2 might function downstream of CgMAVS-like. Moreover, the 
      subcellular localization analysis revealed that CgTRAF2 was localized in 
      cytoplasm, and it may play predominately important roles in signal transduction. 
      Collectively, these results demonstrated that CgTRAF2 might play important roles 
      in the innate immunity and larval development of the Pacific oyster.
CI  - Copyright (c) 2015 Elsevier Ltd. All rights reserved.
FAU - Huang, Baoyu
AU  - Huang B
AD  - National & Local Joint Engineering Laboratory of Ecological Mariculture, 
      Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
FAU - Zhang, Linlin
AU  - Zhang L
AD  - National & Local Joint Engineering Laboratory of Ecological Mariculture, 
      Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
FAU - Du, Yishuai
AU  - Du Y
AD  - National & Local Joint Engineering Laboratory of Ecological Mariculture, 
      Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China.
FAU - Li, Li
AU  - Li L
AD  - National & Local Joint Engineering Laboratory of Ecological Mariculture, 
      Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. 
      Electronic address: lili@qdio.ac.cn.
FAU - Tang, Xueying
AU  - Tang X
AD  - National & Local Joint Engineering Laboratory of Ecological Mariculture, 
      Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China; 
      University of Chinese Academy of Sciences, Beijing 100049, China.
FAU - Zhang, Guofan
AU  - Zhang G
AD  - National & Local Joint Engineering Laboratory of Ecological Mariculture, 
      Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China. 
      Electronic address: gfzhang@qdio.ac.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151124
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (DNA, Complementary)
RN  - 0 (RNA, Messenger)
RN  - 0 (TNF Receptor-Associated Factor 2)
SB  - IM
MH  - Animals
MH  - Crassostrea/*genetics/microbiology/virology
MH  - DNA, Complementary/genetics
MH  - Gastrointestinal Tract/metabolism
MH  - Gills/metabolism
MH  - Hemocytes/metabolism
MH  - Herpesviridae
MH  - Herpesviridae Infections/genetics/veterinary
MH  - Molecular Sequence Data
MH  - Phylogeny
MH  - RNA, Messenger/metabolism
MH  - Real-Time Polymerase Chain Reaction
MH  - Sequence Analysis, DNA
MH  - TNF Receptor-Associated Factor 2/*genetics
MH  - Up-Regulation
MH  - Vibrio Infections/genetics/veterinary
MH  - Vibrio alginolyticus
OTO - NOTNLM
OT  - Crassostrea gigas
OT  - Innate immunity
OT  - MAVS
OT  - Pacific oyster
OT  - TRAF2
EDAT- 2015/12/02 06:00
MHDA- 2017/02/02 06:00
CRDT- 2015/12/02 06:00
PHST- 2015/07/31 00:00 [received]
PHST- 2015/11/02 00:00 [revised]
PHST- 2015/11/22 00:00 [accepted]
PHST- 2015/12/02 06:00 [entrez]
PHST- 2015/12/02 06:00 [pubmed]
PHST- 2017/02/02 06:00 [medline]
AID - S1050-4648(15)30246-1 [pii]
AID - 10.1016/j.fsi.2015.11.027 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2016 Jan;48:12-9. doi: 10.1016/j.fsi.2015.11.027. Epub 
      2015 Nov 24.