PMID- 26627460
OWN - NLM
STAT- MEDLINE
DCOM- 20171023
LR  - 20230203
IS  - 1935-3456 (Electronic)
IS  - 1933-0219 (Print)
IS  - 1933-0219 (Linking)
VI  - 9
IP  - 5
DP  - 2016 Sep
TI  - Lung epithelium and myeloid cells cooperate to clear acute pneumococcal 
      infection.
PG  - 1288-302
LID - 10.1038/mi.2015.128 [doi]
AB  - The Gram-positive bacterium Streptococcus pneumoniae causes life-threatening 
      infections, especially among immunocompromised patients. The host's immune system 
      senses S. pneumoniae via different families of pattern recognition receptors, in 
      particular the Toll-like receptor (TLR) family that promotes immune cell 
      activation. Yet, while single TLRs are dispensable for initiating inflammatory 
      responses against S. pneumoniae, the central TLR adapter protein myeloid 
      differentiation factor 88 (MyD88) is of vital importance, as MyD88-deficient mice 
      succumb rapidly to infection. Since MyD88 is ubiquitously expressed in 
      hematopoietic and non-hematopoietic cells, the extent to which MyD88 signaling is 
      required in different cell types to control S. pneumoniae is unknown. Therefore, 
      we used novel conditional knockin mice to investigate the necessity of MyD88 
      signaling in distinct lung-resident myeloid and epithelial cells for the 
      initiation of a protective immune response against S. pneumoniae. Here, we show 
      that MyD88 signaling in lysozyme M (LysM)- and CD11c-expressing myeloid cells, as 
      well as in pulmonary epithelial cells, is critical to restore inflammatory 
      cytokine and antimicrobial peptide production, leading to efficient neutrophil 
      recruitment and enhanced bacterial clearance. Overall, we show a novel 
      synergistic requirement of compartment-specific MyD88 signaling in S. pneumoniae 
      immunity.
FAU - Dudek, M
AU  - Dudek M
AD  - Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical 
      Infection Research, a joint venture between the Medical School Hannover (MHH) and 
      the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
FAU - Puttur, F
AU  - Puttur F
AD  - Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical 
      Infection Research, a joint venture between the Medical School Hannover (MHH) and 
      the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
FAU - Arnold-Schrauf, C
AU  - Arnold-Schrauf C
AD  - Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical 
      Infection Research, a joint venture between the Medical School Hannover (MHH) and 
      the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
FAU - Kuhl, A A
AU  - Kuhl AA
AD  - Department of Medicine I for Gastroenterology, Infectious Disease and 
      Rheumatology, Campus Benjamin Franklin, Charite-Universitatsmedizin, Berlin, 
      Germany.
FAU - Holzmann, B
AU  - Holzmann B
AD  - Department of Surgery, Technische Universitat Munchen, Munich, Germany.
FAU - Henriques-Normark, B
AU  - Henriques-Normark B
AD  - Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, 
      Stockholm, Sweden.
AD  - Department of Clinical Microbiology, Karolinska University Hospital, Stockholm, 
      Sweden.
FAU - Berod, L
AU  - Berod L
AD  - Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical 
      Infection Research, a joint venture between the Medical School Hannover (MHH) and 
      the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
FAU - Sparwasser, T
AU  - Sparwasser T
AD  - Institute of Infection Immunology, TWINCORE, Centre for Experimental and Clinical 
      Infection Research, a joint venture between the Medical School Hannover (MHH) and 
      the Helmholtz Centre for Infection Research (HZI), Hannover, Germany.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20151202
PL  - United States
TA  - Mucosal Immunol
JT  - Mucosal immunology
JID - 101299742
RN  - 0 (CD11c Antigen)
RN  - 0 (Myd88 protein, mouse)
RN  - 0 (Myeloid Differentiation Factor 88)
RN  - EC 3.2.1.17 (Muramidase)
RN  - EC 3.2.1.17 (lysozyme M, mouse)
SB  - IM
MH  - Animals
MH  - CD11c Antigen/genetics/immunology
MH  - Cell Communication/immunology
MH  - Epithelial Cells/*immunology/microbiology
MH  - Gene Expression Regulation
MH  - Gene Knock-In Techniques
MH  - Lung/*immunology/microbiology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Transgenic
MH  - Muramidase/genetics/immunology
MH  - Myeloid Differentiation Factor 88/genetics/*immunology
MH  - Neutrophil Infiltration
MH  - Neutrophils/*immunology/microbiology
MH  - Pneumonia, Pneumococcal/genetics/*immunology/microbiology
MH  - Signal Transduction
MH  - Streptococcus pneumoniae/growth & development/immunology/pathogenicity
PMC - PMC4990776
EDAT- 2015/12/03 06:00
MHDA- 2017/10/24 06:00
PMCR- 2016/08/19
CRDT- 2015/12/03 06:00
PHST- 2015/07/01 00:00 [received]
PHST- 2015/11/01 00:00 [accepted]
PHST- 2015/12/03 06:00 [entrez]
PHST- 2015/12/03 06:00 [pubmed]
PHST- 2017/10/24 06:00 [medline]
PHST- 2016/08/19 00:00 [pmc-release]
AID - S1933-0219(22)00773-5 [pii]
AID - 10.1038/mi.2015.128 [doi]
PST - ppublish
SO  - Mucosal Immunol. 2016 Sep;9(5):1288-302. doi: 10.1038/mi.2015.128. Epub 2015 Dec 
      2.